The impact of physiological state and environmental stress on bacterial load estimation methodologies for Mycobacterium tuberculosis

• Published in: Scientific reports Vol. 14; no. 1; pp. 26108 - 8
• Main Authors: Maitra, Arundhati, Wijk, Marie, Margaryan, Hasmik, Denti, Paolo, McHugh, Timothy D., Kloprogge, Frank
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.10.2024; Nature Portfolio
• Subjects: 631/326/22/1290; 631/326/2521; 692/308/2778; 692/53/2421; 692/699/255/1856; Bacterial Load; Colony Count, Microbial; Early bactericidal activity; Humanities and Social Sciences; Humans; Isoniazid - pharmacology; multidisciplinary; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - growth & development; Mycobacterium tuberculosis - physiology; Rifampin - pharmacology; Science; Science (multidisciplinary); Stress, Physiological; Tuberculosis; Tuberculosis - drug therapy; Tuberculosis - microbiology; Early bactericidal activity; Tuberculosis

When processed in solid or liquid medium, tuberculosis patient samples yield different proportions of a heterogenous bacterial community over the duration of treatment. We aimed to derive a relationship between methodologies for bacterial load determination and assess the effect of the growth phase...