The regulation of TNFα production after heat and endotoxin stimulation is dependent on Annexin-A1 and HSP70

• Published in: Cell stress & chaperones Vol. 20; no. 4; pp. 583 - 593
• Main Authors: Nair, Sunitha, Arora, Suruchi, Lim, Jyue Yuan, Lee, Lay Hoon, Lim, Lina H.K.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.07.2015; Springer Netherlands
• Subjects: Actins; Animals; Annexin A1 - deficiency; Annexin A1 - genetics; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bone marrow cells; Bone Marrow Cells - cytology; Cancer Research; Cell Biology; Cell lines; Cells, Cultured; Cytokines; Cytokines - analysis; Enzyme-Linked Immunosorbent Assay; Extracellular Signal-Regulated MAP Kinases - metabolism; Gene expression regulation; Heat stress disorders; HSP70 Heat-Shock Proteins - metabolism; Immunology; JNK Mitogen-Activated Protein Kinases - metabolism; Lipopolysaccharides - toxicity; Macrophages; Macrophages - cytology; Macrophages - drug effects; Macrophages - metabolism; Messenger RNA; Mice; Mice, Inbred BALB C; Mice, Knockout; Neurosciences; Original Paper; Physiological regulation; Real-Time Polymerase Chain Reaction; RNA Stability - drug effects; Shock heating; Signal Transduction - drug effects; Temperature; Toll-Like Receptors - agonists; Toll-Like Receptors - metabolism; Tumor Necrosis Factor-alpha - analysis; Tumor Necrosis Factor-alpha - genetics; Heat; Inflammation; HSP70; TNF; Annexin-A1

Febrile temperatures can induce stress responses which protect cells from damage and can reduce inflammation during infections and sepsis. However, the mechanisms behind the protective functions of heat in response to the bacterial endotoxin LPS are unclear. We have recently shown that Annexin-1 (AN...