42Sp48 in Previtellogenic Xenopus Oocytes Is Structurally Homologous to EF-1α and May Be a Stage-Specific Elongation Factor

• Published in: The Journal of cell biology Vol. 112; no. 2; pp. 237 - 243
• Main Authors: Coppard, Nicholas J., Poulsen, Knud, Madsen, Hans O., Frydenberg, Jane, Brian F. C. Clark
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.01.1991; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Amino acids; Analytical, structural and metabolic biochemistry; Animals; Base Sequence; Binding and carrier proteins; Biological and medical sciences; Blotting, Southern; CDNA libraries; Complementary DNA; DNA; DNA - genetics; DNA - isolation & purification; DNA probes; elongation factor EF-1 alpha; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Genes; Genomics; Molecular Sequence Data; Mutation; Oocytes; Open reading frames; Peptide Elongation Factor 1; Peptide elongation factors; Peptide Elongation Factors - genetics; Proteins; Sequence Homology, Nucleic Acid; Transfer RNA; Xenopus laevis; Xenopus laevis - genetics; Xenopus Proteins; Nucleotide sequence; Xenopus laevis; Prediction; Amphibia; Homology; Salientia; Binding protein; Vertebrata; Complementary DNA; Southern blotting; Nucleic acid; Elongation factor EF1α; Oocyte; Aminoacid sequence

We have isolated the cDNA for 42Sp48 and EF-1α from mixed stage oocytes and tailbud (stage 22) Xenopus laevis cDNA libraries by use of the cDNA for human elongation factor-1α (EF-1α) as probe. The nucleotide and deduced amino acid sequences of the entire coding region of 42Sp48 and EF-1α cDNA were e...