The role of c‐kit‐positive interstitial cells in mediating phasic contractions of bladder strips from streptozotocin‐induced diabetic rats
What’s known on the subject? and What does the study add? Although the functional significance of ICCs in the bladder is still not clear, they have been linked with detrusor overactivity. In this study we demonstrate the presence of c‐kit positive interstitial cells in rat urinary bladder for the fi...
Saved in:
| Published in | BJU international Vol. 107; no. 9; pp. 1480 - 1487 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Oxford, UK
Blackwell Publishing Ltd
01.05.2011
Wiley-Blackwell |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1464-4096 1464-410X 1464-410X |
| DOI | 10.1111/j.1464-410X.2010.09507.x |
Cover
| Abstract | What’s known on the subject? and What does the study add?
Although the functional significance of ICCs in the bladder is still not clear, they have been linked with detrusor overactivity. In this study we demonstrate the presence of c‐kit positive interstitial cells in rat urinary bladder for the first time and their importance in mediating muscarinic receptor‐induced phasic contractions of bladder strips from control and diabetic rats. The role of these cells does not seem to be significantly altered by the diabetic state.
OBJECTIVE
•
To investigate the role of c‐kit‐positive interstitial cells (ICCs) in mediating muscarinic receptor‐induced phasic contractions of isolated bladder strips from streptozotocin(STZ)‐induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder.
MATERIALS AND METHODS
•
Bladders were removed from STZ‐induced diabetic rats at 1, 4 and 12 weeks after induction of diabetes and from age‐matched controls.
•
To investigate the functional role of ICCs in mediating phasic contractions, bladder strips were isolated from control and diabetic rats and mounted in tissue baths.
•
Strips were stimulated with low concentrations of the muscarinic receptor agonist carbachol (CCH; 0.1 µm) to induce phasic contractions and the effect of increasing concentrations (1–50 µm) of imatinib (Glivec® or Gleevec®, formerly STI571), a c‐kit tyrosine kinase inhibitor, was then investigated.
•
For molecular studies, to detect expression of the c‐kit tyrosine kinase receptor (c‐kit), total cellular RNA was extracted from rat bladders and reverse‐transcribed to obtain complementary DNA (cDNA).
•
Reverse transcription‐polymerase chain reaction (RT‐PCR) was then performed using primers specific to the c‐kit sequence and amplified products separated by agarose gel electrophoresis.
•
Amplified PCR products were excised from the gel, sequenced and compared with the known c‐kit sequence to confirm their identity.
•
For immunohistochemical detection, whole mount preparations of control rat bladders were fixed in acetone and labelled using antibodies directed to the ICC marker c‐kit.
RESULTS
•
In functional studies, CCH induced phasic contractions in bladder strips from control and diabetic rats. Bladder strips from 1‐week diabetic rats showed CCH‐induced phasic contractions, which were greater in amplitude, but had lower frequency, than the controls, whilst no such differences were apparent at later time points of diabetes.
•
Imatinib decreased the amplitude and the frequency of the CCH‐induced phasic contractions in both control and diabetic tissues in a concentration‐dependent manner, although in diabetic tissues this effect was only seen at the higher concentrations of imatinib. RT‐PCR of bladder cDNA yielded a single amplicon of 480 bp.
•
The sequence of this amplicon showed a 98% homology with the published c‐kit sequence, thus confirming c‐kit mRNA expression in both control and 1‐week diabetic rat bladder.
•
Expression of c‐kit protein was also detected in a network of cells on the edge of and between smooth muscle bundles of control rat bladders by positive immunoreactivity to c‐kit specific antibodies.
CONCLUSION
•
These data show the presence of c‐kit‐positive ICCs in rat urinary bladder and their importance in mediating muscarinic receptor‐induced phasic contractions of bladder strips from control and diabetic rats. The role of these ICCs does not seem to be significantly altered by the diabetic state. |
|---|---|
| AbstractList | What’s known on the subject? and What does the study add?
Although the functional significance of ICCs in the bladder is still not clear, they have been linked with detrusor overactivity. In this study we demonstrate the presence of c‐kit positive interstitial cells in rat urinary bladder for the first time and their importance in mediating muscarinic receptor‐induced phasic contractions of bladder strips from control and diabetic rats. The role of these cells does not seem to be significantly altered by the diabetic state.
OBJECTIVE
•
To investigate the role of c‐kit‐positive interstitial cells (ICCs) in mediating muscarinic receptor‐induced phasic contractions of isolated bladder strips from streptozotocin(STZ)‐induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder.
MATERIALS AND METHODS
•
Bladders were removed from STZ‐induced diabetic rats at 1, 4 and 12 weeks after induction of diabetes and from age‐matched controls.
•
To investigate the functional role of ICCs in mediating phasic contractions, bladder strips were isolated from control and diabetic rats and mounted in tissue baths.
•
Strips were stimulated with low concentrations of the muscarinic receptor agonist carbachol (CCH; 0.1 µm) to induce phasic contractions and the effect of increasing concentrations (1–50 µm) of imatinib (Glivec® or Gleevec®, formerly STI571), a c‐kit tyrosine kinase inhibitor, was then investigated.
•
For molecular studies, to detect expression of the c‐kit tyrosine kinase receptor (c‐kit), total cellular RNA was extracted from rat bladders and reverse‐transcribed to obtain complementary DNA (cDNA).
•
Reverse transcription‐polymerase chain reaction (RT‐PCR) was then performed using primers specific to the c‐kit sequence and amplified products separated by agarose gel electrophoresis.
•
Amplified PCR products were excised from the gel, sequenced and compared with the known c‐kit sequence to confirm their identity.
•
For immunohistochemical detection, whole mount preparations of control rat bladders were fixed in acetone and labelled using antibodies directed to the ICC marker c‐kit.
RESULTS
•
In functional studies, CCH induced phasic contractions in bladder strips from control and diabetic rats. Bladder strips from 1‐week diabetic rats showed CCH‐induced phasic contractions, which were greater in amplitude, but had lower frequency, than the controls, whilst no such differences were apparent at later time points of diabetes.
•
Imatinib decreased the amplitude and the frequency of the CCH‐induced phasic contractions in both control and diabetic tissues in a concentration‐dependent manner, although in diabetic tissues this effect was only seen at the higher concentrations of imatinib. RT‐PCR of bladder cDNA yielded a single amplicon of 480 bp.
•
The sequence of this amplicon showed a 98% homology with the published c‐kit sequence, thus confirming c‐kit mRNA expression in both control and 1‐week diabetic rat bladder.
•
Expression of c‐kit protein was also detected in a network of cells on the edge of and between smooth muscle bundles of control rat bladders by positive immunoreactivity to c‐kit specific antibodies.
CONCLUSION
•
These data show the presence of c‐kit‐positive ICCs in rat urinary bladder and their importance in mediating muscarinic receptor‐induced phasic contractions of bladder strips from control and diabetic rats. The role of these ICCs does not seem to be significantly altered by the diabetic state. • To investigate the role of c-kit-positive interstitial cells (ICCs) in mediating muscarinic receptor-induced phasic contractions of isolated bladder strips from streptozotocin(STZ)-induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder. • Bladders were removed from STZ-induced diabetic rats at 1, 4 and 12 weeks after induction of diabetes and from age-matched controls. • To investigate the functional role of ICCs in mediating phasic contractions, bladder strips were isolated from control and diabetic rats and mounted in tissue baths. • Strips were stimulated with low concentrations of the muscarinic receptor agonist carbachol (CCH; 0.1 µm) to induce phasic contractions and the effect of increasing concentrations (1-50 µm) of imatinib (Glivec® or Gleevec®, formerly STI571), a c-kit tyrosine kinase inhibitor, was then investigated. • For molecular studies, to detect expression of the c-kit tyrosine kinase receptor (c-kit), total cellular RNA was extracted from rat bladders and reverse-transcribed to obtain complementary DNA (cDNA). • Reverse transcription-polymerase chain reaction (RT-PCR) was then performed using primers specific to the c-kit sequence and amplified products separated by agarose gel electrophoresis. • Amplified PCR products were excised from the gel, sequenced and compared with the known c-kit sequence to confirm their identity. • For immunohistochemical detection, whole mount preparations of control rat bladders were fixed in acetone and labelled using antibodies directed to the ICC marker c-kit. • In functional studies, CCH induced phasic contractions in bladder strips from control and diabetic rats. Bladder strips from 1-week diabetic rats showed CCH-induced phasic contractions, which were greater in amplitude, but had lower frequency, than the controls, whilst no such differences were apparent at later time points of diabetes. • Imatinib decreased the amplitude and the frequency of the CCH-induced phasic contractions in both control and diabetic tissues in a concentration-dependent manner, although in diabetic tissues this effect was only seen at the higher concentrations of imatinib. RT-PCR of bladder cDNA yielded a single amplicon of 480 bp. • The sequence of this amplicon showed a 98% homology with the published c-kit sequence, thus confirming c-kit mRNA expression in both control and 1-week diabetic rat bladder. • Expression of c-kit protein was also detected in a network of cells on the edge of and between smooth muscle bundles of control rat bladders by positive immunoreactivity to c-kit specific antibodies. • These data show the presence of c-kit-positive ICCs in rat urinary bladder and their importance in mediating muscarinic receptor-induced phasic contractions of bladder strips from control and diabetic rats. The role of these ICCs does not seem to be significantly altered by the diabetic state. • To investigate the role of c-kit-positive interstitial cells (ICCs) in mediating muscarinic receptor-induced phasic contractions of isolated bladder strips from streptozotocin(STZ)-induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder.OBJECTIVE• To investigate the role of c-kit-positive interstitial cells (ICCs) in mediating muscarinic receptor-induced phasic contractions of isolated bladder strips from streptozotocin(STZ)-induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder.• Bladders were removed from STZ-induced diabetic rats at 1, 4 and 12 weeks after induction of diabetes and from age-matched controls. • To investigate the functional role of ICCs in mediating phasic contractions, bladder strips were isolated from control and diabetic rats and mounted in tissue baths. • Strips were stimulated with low concentrations of the muscarinic receptor agonist carbachol (CCH; 0.1 µm) to induce phasic contractions and the effect of increasing concentrations (1-50 µm) of imatinib (Glivec® or Gleevec®, formerly STI571), a c-kit tyrosine kinase inhibitor, was then investigated. • For molecular studies, to detect expression of the c-kit tyrosine kinase receptor (c-kit), total cellular RNA was extracted from rat bladders and reverse-transcribed to obtain complementary DNA (cDNA). • Reverse transcription-polymerase chain reaction (RT-PCR) was then performed using primers specific to the c-kit sequence and amplified products separated by agarose gel electrophoresis. • Amplified PCR products were excised from the gel, sequenced and compared with the known c-kit sequence to confirm their identity. • For immunohistochemical detection, whole mount preparations of control rat bladders were fixed in acetone and labelled using antibodies directed to the ICC marker c-kit.MATERIALS AND METHODS• Bladders were removed from STZ-induced diabetic rats at 1, 4 and 12 weeks after induction of diabetes and from age-matched controls. • To investigate the functional role of ICCs in mediating phasic contractions, bladder strips were isolated from control and diabetic rats and mounted in tissue baths. • Strips were stimulated with low concentrations of the muscarinic receptor agonist carbachol (CCH; 0.1 µm) to induce phasic contractions and the effect of increasing concentrations (1-50 µm) of imatinib (Glivec® or Gleevec®, formerly STI571), a c-kit tyrosine kinase inhibitor, was then investigated. • For molecular studies, to detect expression of the c-kit tyrosine kinase receptor (c-kit), total cellular RNA was extracted from rat bladders and reverse-transcribed to obtain complementary DNA (cDNA). • Reverse transcription-polymerase chain reaction (RT-PCR) was then performed using primers specific to the c-kit sequence and amplified products separated by agarose gel electrophoresis. • Amplified PCR products were excised from the gel, sequenced and compared with the known c-kit sequence to confirm their identity. • For immunohistochemical detection, whole mount preparations of control rat bladders were fixed in acetone and labelled using antibodies directed to the ICC marker c-kit.• In functional studies, CCH induced phasic contractions in bladder strips from control and diabetic rats. Bladder strips from 1-week diabetic rats showed CCH-induced phasic contractions, which were greater in amplitude, but had lower frequency, than the controls, whilst no such differences were apparent at later time points of diabetes. • Imatinib decreased the amplitude and the frequency of the CCH-induced phasic contractions in both control and diabetic tissues in a concentration-dependent manner, although in diabetic tissues this effect was only seen at the higher concentrations of imatinib. RT-PCR of bladder cDNA yielded a single amplicon of 480 bp. • The sequence of this amplicon showed a 98% homology with the published c-kit sequence, thus confirming c-kit mRNA expression in both control and 1-week diabetic rat bladder. • Expression of c-kit protein was also detected in a network of cells on the edge of and between smooth muscle bundles of control rat bladders by positive immunoreactivity to c-kit specific antibodies.RESULTS• In functional studies, CCH induced phasic contractions in bladder strips from control and diabetic rats. Bladder strips from 1-week diabetic rats showed CCH-induced phasic contractions, which were greater in amplitude, but had lower frequency, than the controls, whilst no such differences were apparent at later time points of diabetes. • Imatinib decreased the amplitude and the frequency of the CCH-induced phasic contractions in both control and diabetic tissues in a concentration-dependent manner, although in diabetic tissues this effect was only seen at the higher concentrations of imatinib. RT-PCR of bladder cDNA yielded a single amplicon of 480 bp. • The sequence of this amplicon showed a 98% homology with the published c-kit sequence, thus confirming c-kit mRNA expression in both control and 1-week diabetic rat bladder. • Expression of c-kit protein was also detected in a network of cells on the edge of and between smooth muscle bundles of control rat bladders by positive immunoreactivity to c-kit specific antibodies.• These data show the presence of c-kit-positive ICCs in rat urinary bladder and their importance in mediating muscarinic receptor-induced phasic contractions of bladder strips from control and diabetic rats. The role of these ICCs does not seem to be significantly altered by the diabetic state.CONCLUSION• These data show the presence of c-kit-positive ICCs in rat urinary bladder and their importance in mediating muscarinic receptor-induced phasic contractions of bladder strips from control and diabetic rats. The role of these ICCs does not seem to be significantly altered by the diabetic state. |
| Author | McKay, Neil G. Vahabi, Bahareh Sellers, Donna J. Lawson, Kim |
| Author_xml | – sequence: 1 givenname: Bahareh surname: Vahabi fullname: Vahabi, Bahareh – sequence: 2 givenname: Neil G. surname: McKay fullname: McKay, Neil G. – sequence: 3 givenname: Kim surname: Lawson fullname: Lawson, Kim – sequence: 4 givenname: Donna J. surname: Sellers fullname: Sellers, Donna J. |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24091631$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/20735390$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkc1uVCEUx4lpYz_0FQwb42pGuNx7YRaaaNOqTRM3beKOcLkHy8jAFRj7sfIR-ow-ieDMaOKqLOBw-J0_Oed_hPZ88IAQpmROy3q9nNO2b2ctJV_mDSlZsugIn98-QYd_H_Z2MVn0B-gopSUhJdF3T9FBQzjr2IIcoofLa8AxOMDBYP3r58M3m8s-hWSz_QHY-gwx5XJRDmtwLpUUXsFoVbb-K56uVbIa6-BzVDrb4FNVGpwaR4g45WinhE0MqxrDlMN9yEFbXz6xflxrGHHRGiAXlahyeob2jXIJnm_PY3R1dnp58nF28fnDp5N3FzNdmuKzZgBCBOdGs2YwY6NYy4QZemH6saWN4g1vQdOOdVz0XBhohQY1kk6PZGiUYMfo1UZ3iuH7GlKWK5tqg8pDWCcpesZ70QleyBdbcj2UxuUU7UrFO7kbYgFebgGVtHImKq9t-scVB2jPaOHebjgdQ0oRjNQ2qzqzMjvrJCWyuiuXshonq4myuiv_uCtvi4D4T2D3xyNK32xKb6yDu0fXyffnVzVivwFQBsJv |
| CitedBy_id | crossref_primary_10_1016_j_juro_2012_03_117 crossref_primary_10_1111_iju_12491 crossref_primary_10_1016_j_urology_2012_07_031 crossref_primary_10_1155_2017_3457093 crossref_primary_10_1371_journal_pone_0048897 crossref_primary_10_1155_2011_816342 crossref_primary_10_1152_physrev_00037_2013 crossref_primary_10_1038_s12276_018_0088_z crossref_primary_10_1016_j_jss_2011_07_048 crossref_primary_10_4196_kjpp_2013_17_6_531 crossref_primary_10_1038_srep24844 crossref_primary_10_3389_fsurg_2018_00072 crossref_primary_10_1146_annurev_pharmtox_010617_052615 crossref_primary_10_1111_bju_12172 crossref_primary_10_1186_s13287_020_01795_4 crossref_primary_10_1111_apha_12373 crossref_primary_10_1002_nau_22415 crossref_primary_10_1111_1440_1681_13003 crossref_primary_10_1016_j_tem_2016_02_006 crossref_primary_10_1038_emm_2017_31 crossref_primary_10_1111_apha_12009 crossref_primary_10_1371_journal_pone_0058611 crossref_primary_10_1007_s11884_023_00691_w crossref_primary_10_1152_ajprenal_00106_2018 crossref_primary_10_1371_journal_pone_0106190 crossref_primary_10_3389_fnsys_2022_884260 |
| Cites_doi | 10.1111/j.1464-410X.2004.05186.x 10.1097/01.ju.0000125097.25475.17 10.1016/S0022-5347(05)67951-7 10.1016/S0022-5347(05)64752-0 10.1007/s00441-007-0454-y 10.1111/j.1474-8673.2006.00371.x 10.1242/dev.116.2.369 10.1007/s00441-005-1151-3 10.1152/ajpregu.00236.2006 10.1016/j.eururo.2008.11.009 10.1111/j.1464-410X.2009.08423.x 10.1002/nau.20502 10.1111/j.1464-410X.2005.05652.x 10.1111/j.1464-410X.2005.05988.x 10.1097/01.ju.0000146272.80848.37 10.1016/j.ejphar.2006.06.068 10.1111/j.1464-410X.2007.07021.x 10.1113/jphysiol.2004.065136 10.1046/j.1365-201X.2001.00908.x 10.1002/nau.20085 10.1113/eph8802473 10.1111/j.1464-410X.2006.06203.x 10.1046/j.1464-410X.2002.02834.x 10.1159/000092530 10.1182/blood.V96.3.925 10.1152/ajprenal.90315.2008 10.1111/j.1474-8673.2006.00363.x 10.1016/S0022-5347(05)64910-5 10.1016/0306-4522(95)00453-X 10.1053/gast.1996.v111.pm8690216 |
| ContentType | Journal Article |
| Copyright | 2010 THE AUTHORS. JOURNAL COMPILATION © 2010 BJU INTERNATIONAL 2015 INIST-CNRS 2010 THE AUTHORS. JOURNAL COMPILATION © 2010 BJU INTERNATIONAL. |
| Copyright_xml | – notice: 2010 THE AUTHORS. JOURNAL COMPILATION © 2010 BJU INTERNATIONAL – notice: 2015 INIST-CNRS – notice: 2010 THE AUTHORS. JOURNAL COMPILATION © 2010 BJU INTERNATIONAL. |
| DBID | AAYXX CITATION IQODW CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1111/j.1464-410X.2010.09507.x |
| DatabaseName | CrossRef Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1464-410X |
| EndPage | 1487 |
| ExternalDocumentID | 20735390 24091631 10_1111_j_1464_410X_2010_09507_x BJU9507 |
| Genre | article Journal Article |
| GroupedDBID | --- .3N .55 .GA .Y3 05W 0R~ 10A 1OC 23N 24P 2WC 31~ 33P 36B 3O- 3SF 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52R 52S 52T 52U 52V 52W 52X 53G 5GY 5HH 5LA 5RE 5VS 66C 6P2 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A01 A03 AAESR AAEVG AAHHS AAHQN AAIPD AAMNL AANLZ AAONW AASGY AAXRX AAYCA AAZKR ABCQN ABCUV ABDBF ABEML ABJNI ABLJU ABOCM ABPVW ABQWH ABXGK ACAHQ ACCFJ ACCZN ACFBH ACGFS ACGOF ACMXC ACPOU ACPRK ACSCC ACUHS ACXBN ACXQS ADBBV ADBTR ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFEBI AFFNX AFFPM AFGKR AFPWT AFWVQ AFZJQ AHBTC AHMBA AIACR AITYG AIURR AIWBW AJBDE ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN ALVPJ AMBMR AMYDB ATUGU AZBYB AZVAB BAFTC BAWUL BFHJK BHBCM BMXJE BROTX BRXPI BY8 C45 CAG COF CS3 D-6 D-7 D-E D-F DCZOG DIK DPXWK DR2 DRFUL DRMAN DRSTM DU5 E3Z EAD EAP EBC EBD EBS EJD EMB EMK EMOBN ESX EX3 F00 F01 F04 F5P FUBAC G-S G.N GODZA H.X HF~ HGLYW HZI HZ~ IHE IX1 J0M J5H K48 KBYEO LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRMAN MRSTM MSFUL MSMAN MSSTM MXFUL MXMAN MXSTM N04 N05 N9A NF~ O66 O9- OIG OK1 OVD P2P P2W P2X P2Z P4B P4D PQQKQ Q.N Q11 QB0 R.K RJQFR ROL RX1 SUPJJ SV3 TEORI TUS UB1 V9Y W8V W99 WBKPD WHWMO WIH WIJ WIK WOHZO WOW WQJ WRC WVDHM WXI WXSBR X7M XG1 YFH ZGI ZXP ~IA ~WT AAYXX AEYWJ AGHNM AGYGG CITATION AAMMB AEFGJ AGXDD AIDQK AIDYY IQODW CGR CUY CVF ECM EIF NPM 1OB 7X8 |
| ID | FETCH-LOGICAL-c4647-2be00877fc32bfd2a3438fb68f6d412a7274ec153578678fe48cead05cd0b2a83 |
| IEDL.DBID | DR2 |
| ISSN | 1464-4096 1464-410X |
| IngestDate | Fri Sep 05 13:14:33 EDT 2025 Wed Feb 19 01:57:03 EST 2025 Mon Jul 21 09:09:57 EDT 2025 Tue Jul 01 03:49:47 EDT 2025 Thu Apr 24 23:03:19 EDT 2025 Wed Jan 22 16:40:24 EST 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 9 |
| Keywords | Endocrinopathy Antineoplastic agent Animal model Nephrology Rat Urology rat bladder Urinary bladder Protein-tyrosine kinase kit Gene Streptozotocin Protooncogene Cell contraction Imatinib Enzyme Tyrosine kinase inhibitor c-kit Transferases Diabetes mellitus Rodentia Enzyme inhibitor interstitial cells Interstitial cell Vertebrata Antibiotic Mammalia Urinary system Animal Reagent strip C-Onc gene diabetes |
| Language | English |
| License | CC BY 4.0 2010 THE AUTHORS. JOURNAL COMPILATION © 2010 BJU INTERNATIONAL. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4647-2be00877fc32bfd2a3438fb68f6d412a7274ec153578678fe48cead05cd0b2a83 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| PMID | 20735390 |
| PQID | 863768587 |
| PQPubID | 23479 |
| PageCount | 1 |
| ParticipantIDs | proquest_miscellaneous_863768587 pubmed_primary_20735390 pascalfrancis_primary_24091631 crossref_citationtrail_10_1111_j_1464_410X_2010_09507_x crossref_primary_10_1111_j_1464_410X_2010_09507_x wiley_primary_10_1111_j_1464_410X_2010_09507_x_BJU9507 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | May 2011 |
| PublicationDateYYYYMMDD | 2011-05-01 |
| PublicationDate_xml | – month: 05 year: 2011 text: May 2011 |
| PublicationDecade | 2010 |
| PublicationPlace | Oxford, UK |
| PublicationPlace_xml | – name: Oxford, UK – name: Oxford – name: England |
| PublicationTitle | BJU international |
| PublicationTitleAlternate | BJU Int |
| PublicationYear | 2011 |
| Publisher | Blackwell Publishing Ltd Wiley-Blackwell |
| Publisher_xml | – name: Blackwell Publishing Ltd – name: Wiley-Blackwell |
| References | 2005; 173 2006; 97 2007; 100 1996; 71 2006; 291 2009; 55 2004; 94 2001; 173 2006; 62 2005; 321 2002; 168 2007; 330 2000; 96 2006; 25 2008; 27 2004; 171 2006; 26 1992; 116 2005; 96 2000; 163 1996; 111 2002; 90 2004; 559 2006; 546 2008; 295 2009; 104 2003; 88 e_1_2_8_27_2 e_1_2_8_28_2 e_1_2_8_29_2 e_1_2_8_23_2 e_1_2_8_24_2 e_1_2_8_25_2 e_1_2_8_26_2 e_1_2_8_9_2 e_1_2_8_2_2 e_1_2_8_4_2 e_1_2_8_3_2 e_1_2_8_6_2 e_1_2_8_5_2 e_1_2_8_8_2 e_1_2_8_7_2 e_1_2_8_20_2 e_1_2_8_21_2 e_1_2_8_22_2 e_1_2_8_16_2 e_1_2_8_17_2 e_1_2_8_18_2 e_1_2_8_19_2 e_1_2_8_12_2 e_1_2_8_13_2 e_1_2_8_14_2 e_1_2_8_15_2 e_1_2_8_31_2 e_1_2_8_30_2 e_1_2_8_10_2 e_1_2_8_11_2 |
| References_xml | – volume: 100 start-page: 668 year: 2007 end-page: 78 article-title: Cholinergic activation of phasic activity in the isolated bladder: possible evidence for M3‐ and M2‐dependent components of a motor/sensory system publication-title: BJU Int – volume: 546 start-page: 177 year: 2006 end-page: 81 article-title: Imatinib inhibits spontaneous rhythmic contractions of human uterus and intestine publication-title: Eur J Pharmacol – volume: 163 start-page: 645 year: 2000 end-page: 51 article-title: Studies of the pathophysiology of the idiopathic detrusor instability: the physiological properties of the detrusor muscle and its pattern of innervation publication-title: J Urol – volume: 171 start-page: 2492 year: 2004 end-page: 6 article-title: Identification of kit positive cells in the human urinary bladder publication-title: J Urol – volume: 97 start-page: 612 year: 2006 end-page: 6 article-title: The functional effects of a c‐kit tyrosine inhibitor on guinea‐pig and human detrusor publication-title: BJU Int – volume: 96 start-page: 22 issue: 1 year: 2005 end-page: 8 article-title: A developing view of the origins of urgency: the importance of animal models publication-title: BJU Int – volume: 96 start-page: 925 year: 2000 end-page: 32 article-title: Inhibition of c‐kit receptor tyrosine kinase activity by STI 571, a selective tyrosine kinase inhibitor publication-title: Blood – volume: 173 start-page: 323 year: 2001 end-page: 33 article-title: Effects of different types of K channel modulators on the spontaneous myogenic contraction of guinea‐pig urinary bladder smoothmuscle publication-title: Acta Physiol Scand – volume: 168 start-page: 832 year: 2002 end-page: 6 article-title: Kit positive cells in the guinea pig bladder publication-title: J Urol – volume: 90 start-page: 118 year: 2002 end-page: 29 article-title: Gap junctions and connexion expression in human suburothelial interstitial cells publication-title: BJU Int – volume: 321 start-page: 341 year: 2005 end-page: 51 article-title: Expression of neuronal nitric oxide synthase (nNOS) and nitric‐oxide‐induced changes in cGMP in the urothelial layer of the guinea pig bladder publication-title: Cell Tissue Res – volume: 559 start-page: 567 year: 2004 end-page: 81 article-title: Role of interstitial cells and gap junctions in the transmission of spontaneous Ca signals in the detrusor smooth muscles of the guinea‐pig urinary bladder publication-title: J Physiol – volume: 295 start-page: F454 year: 2008 end-page: 61 article-title: Urotheliogenic modulation of intrinsic activity in spinal cord‐transected rat bladders: role of urothelial muscarinic receptors publication-title: Am J Physiol Renal Physiol – volume: 168 start-page: 293 year: 2002 end-page: 7 article-title: Topography of the vanilloid receptor in the human bladder: more than just the nerve fibers publication-title: J Urol – volume: 291 start-page: R1049 year: 2006 end-page: 59 article-title: Muscarinic regulation of neonatal rat bladder spontaneous contractions publication-title: Am J Physiol Regul Integr Comp Physiol – volume: 26 start-page: 261 year: 2006 end-page: 6 article-title: Muscarinic stimulation of rat isolated whole bladder: pathophysiological models of detrusor overactivity publication-title: Auton Autacoid Pharmacol – volume: 62 start-page: 79 year: 2006 end-page: 83 article-title: The effect of imatinib mesylate on the contractility of isolated rabbit myometrial strips publication-title: Gynecol Obstet Invest – volume: 71 start-page: 337 year: 1996 end-page: 48 article-title: Distribution of nitric oxide synthase‐immunoreactive nerves and identification of the cellular targets of nitric oxide in guinea‐pig and human urinary bladder by cGMP immunohistochemistry publication-title: Neuroscience – volume: 111 start-page: 492 year: 1996 end-page: 515 article-title: A case for interstitial cells of Cajal as pacemakers and mediators of neurotransmission in the gastrointestinal tract publication-title: Gastroenterology – volume: 25 start-page: 205 year: 2006 end-page: 10 article-title: Effects of imatinib mesylate (Glivec) as a c‐kit tyrosine kinase inhibitor in the guinea‐pig urinary bladder publication-title: Neurourol Urodyn – volume: 26 start-page: 303 year: 2006 end-page: 9 article-title: Muscarinic receptor function: density and G‐protein coupling in the overactive diabetic rat bladder publication-title: Auton Autacoid Pharmacol – volume: 116 start-page: 369 year: 1992 end-page: 75 article-title: Requirement of c‐kit for development of intestinal pacemaker system publication-title: Development – volume: 104 start-page: 398 year: 2009 end-page: 405 article-title: M(3) muscarinic receptor expression on suburothelial interstitial cells publication-title: BJU Int – volume: 88 start-page: 19 year: 2003 end-page: 30 article-title: Autonomous activity in the isolated guinea pig bladder publication-title: Exp Physiol – volume: 55 start-page: 1440 year: 2009 end-page: 8 article-title: Suburothelial myofibroblasts in the human overactive bladder and the effect of botulinum neurotoxin type A treatment publication-title: Eur Urol – volume: 27 start-page: 330 year: 2008 end-page: 40 article-title: Altered distribution of interstitial cells in the guinea pig bladder following bladder outlet obstruction publication-title: Neurourol Urodyn – volume: 97 start-page: 1332 year: 2006 end-page: 7 article-title: Location of interstitial cells and neurotransmitters in the mouse bladder publication-title: BJU Int – volume: 173 start-page: 1385 year: 2005 end-page: 90 article-title: Morphology and localisation of interstitial cells in the guinea pig bladder: structural relationships with smooth muscle and neurons publication-title: J Urol – volume: 330 start-page: 147 year: 2007 end-page: 60 article-title: Alterations to network of NO/cGMP‐responsive interstitial cells induced by outlet obstruction in guinea‐pig bladder publication-title: Cell Tissue Res – volume: 94 start-page: 1114 year: 2004 end-page: 24 article-title: cGMP‐generating cells in the bladder wall: identification of distinct network of interstitial cells publication-title: BJU Int – ident: e_1_2_8_5_2 doi: 10.1111/j.1464-410X.2004.05186.x – ident: e_1_2_8_11_2 doi: 10.1097/01.ju.0000125097.25475.17 – ident: e_1_2_8_20_2 doi: 10.1016/S0022-5347(05)67951-7 – ident: e_1_2_8_9_2 doi: 10.1016/S0022-5347(05)64752-0 – ident: e_1_2_8_24_2 doi: 10.1007/s00441-007-0454-y – ident: e_1_2_8_22_2 doi: 10.1111/j.1474-8673.2006.00371.x – ident: e_1_2_8_13_2 doi: 10.1242/dev.116.2.369 – ident: e_1_2_8_6_2 doi: 10.1007/s00441-005-1151-3 – ident: e_1_2_8_3_2 doi: 10.1152/ajpregu.00236.2006 – ident: e_1_2_8_25_2 doi: 10.1016/j.eururo.2008.11.009 – ident: e_1_2_8_28_2 doi: 10.1111/j.1464-410X.2009.08423.x – ident: e_1_2_8_23_2 doi: 10.1002/nau.20502 – ident: e_1_2_8_27_2 doi: 10.1111/j.1464-410X.2005.05652.x – ident: e_1_2_8_16_2 doi: 10.1111/j.1464-410X.2005.05988.x – ident: e_1_2_8_10_2 doi: 10.1097/01.ju.0000146272.80848.37 – ident: e_1_2_8_19_2 doi: 10.1016/j.ejphar.2006.06.068 – ident: e_1_2_8_4_2 doi: 10.1111/j.1464-410X.2007.07021.x – ident: e_1_2_8_14_2 doi: 10.1113/jphysiol.2004.065136 – ident: e_1_2_8_26_2 doi: 10.1046/j.1365-201X.2001.00908.x – ident: e_1_2_8_18_2 doi: 10.1002/nau.20085 – ident: e_1_2_8_2_2 doi: 10.1113/eph8802473 – ident: e_1_2_8_12_2 doi: 10.1111/j.1464-410X.2006.06203.x – ident: e_1_2_8_29_2 doi: 10.1046/j.1464-410X.2002.02834.x – ident: e_1_2_8_17_2 doi: 10.1159/000092530 – ident: e_1_2_8_15_2 doi: 10.1182/blood.V96.3.925 – ident: e_1_2_8_31_2 doi: 10.1152/ajprenal.90315.2008 – ident: e_1_2_8_21_2 doi: 10.1111/j.1474-8673.2006.00363.x – ident: e_1_2_8_30_2 doi: 10.1016/S0022-5347(05)64910-5 – ident: e_1_2_8_8_2 doi: 10.1016/0306-4522(95)00453-X – ident: e_1_2_8_7_2 doi: 10.1053/gast.1996.v111.pm8690216 |
| SSID | ssj0014665 |
| Score | 2.1461132 |
| Snippet | What’s known on the subject? and What does the study add?
Although the functional significance of ICCs in the bladder is still not clear, they have been linked... • To investigate the role of c-kit-positive interstitial cells (ICCs) in mediating muscarinic receptor-induced phasic contractions of isolated bladder strips... |
| SourceID | proquest pubmed pascalfrancis crossref wiley |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 1480 |
| SubjectTerms | Animals Benzamides Biological and medical sciences c‐kit diabetes Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Experimental - physiopathology Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance imatinib Imatinib Mesylate Immunohistochemistry interstitial cells Interstitial Cells of Cajal - drug effects Interstitial Cells of Cajal - metabolism Interstitial Cells of Cajal - physiology Male Medical sciences Muscle Contraction - drug effects Muscle Contraction - physiology Muscle, Smooth - drug effects Muscle, Smooth - physiology Nephrology. Urinary tract diseases Piperazines - pharmacology Protein Kinase Inhibitors - pharmacology Proto-Oncogene Proteins c-kit - analysis Pyrimidines - pharmacology rat bladder Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction streptozotocin Urinary Bladder - drug effects Urinary Bladder - physiology Urinary Bladder - physiopathology |
| Title | The role of c‐kit‐positive interstitial cells in mediating phasic contractions of bladder strips from streptozotocin‐induced diabetic rats |
| URI | https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fj.1464-410X.2010.09507.x https://www.ncbi.nlm.nih.gov/pubmed/20735390 https://www.proquest.com/docview/863768587 |
| Volume | 107 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEBYhh1IoTd91H0GHXr3YlmzLx7ZJCIH0ELKwN6EnDRtss_aWkFN-Qn5jfklnLO8GhxxC6WUxi0e25G9mPsvzIOSbrpjyTtiYOeZj8Hg2VonVoO6sBIOYqIpjovDpr-J4zk8W-WKMf8JcmFAfYrvhhpox2GtUcKW7h0rOY54mizFCqwJuM0M-mbICy-gfnG0rScGpQ1fJIAK0fRrU8-hAE0_1olUdLJoP3S4eo6NTdju4p6M9stxMLESlLGfrXs_M9YOaj_9n5q_Iy5HF0u8Bdq_JjqvfkGen43f6t-QW0EcxcJE2npq7m9vlRQ-_IULsj6NYpGKFUQqAforfDjr4iw5ZLBiGTdvfCtBDhzj6kHnR4Uj6Eu3kimKvkbajmBqDx67tm-umB5jVcJGL2gJcLQ27yjAKYLx7R-ZHh-c_j-Ox90NsYG5lnGmH1fJKb1imvc0U40x4XQhfWJ5mCmgXdwbMNVgc8LfecWFAKZLc2ERnSrD3ZLduaveRUK-EMcBcTWEqnldJBe-YNjOcOQtcTOcRKTfPWZqxMDr257iUkxckLnHBJS64HBZcXkUk3Uq2oTjIE2T2J1DaCgK1AqrO0ojQDbYkqDo-A1W7Zt1JUYA3ELkoI_IhYO5eGCx1zqokIsWAnCffjvxxMsejT_8q-Jk8D9vsGAP6hez2q7X7Cjyt1_uDBv4FoZozvA |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9tAEF5KCm2h9P1QH-keepWRtStpdewruGmcQ4nBt2WfJMRIwpJLyak_Ib-xv6QzWtlFIYdQejHCeNba1Tcz367mQch7XTLlnbAxc8zH4PFsrBKrQd1ZAQYxUSXHROH5cT5b8MNlthzaAWEuTKgPsTtwQ83o7TUqOB5IX9VyHvNpshxCtEogNxMglLc58A7ciX3-vqslBb_t-0oGGSDu47Cea0ca-ar7jWph2Xzod3EdIR3z295BHTwkq-3UQlzK-WTT6Ym5uFL18T_N_RF5MBBZ-iEg7zG55aon5M58eFX_lFwCACnGLtLaU_P71-X5WQefIUjsh6NYp2KNgQqgABRfH7TwFe0TWTASmzanCgBE-1D6kHzR4kh6haZyTbHdSNNSzI7Ba9d09UXdAdIq-JOzygJiLQ0HyzAKwLx9RhYHX04-zeKh_UNsYG5FnGqHBfMKb1iqvU0V40x4nQufWz5NFTAv7gxYbDA64HK948KAXiSZsYlOlWDPyV5VV-4loV4JY4C8mtyUPCuTEraZNjWcOQt0TGcRKbYPWpqhNjq26FjJ0R6JS1xwiQsu-wWXPyMy3Uk2oT7IDWT2R1jaCQK7ArbOphGhW3BJ0HZ8Bqpy9aaVIgeHIDJRRORFAN1fYTDWGSuTiOQ9dG58O_Lj4QKvXv2r4Dtyd3YyP5JHX4-_vSb3wqk7hoS-IXvdeuPeAm3r9H6vjn8A0gU32w |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1ba9RAFB6kQhGk3m281HnwNUs2M0kmj1ZdarVFxIV9G-aKZUsSNlmRPvkT-hv9JT0nk11J6UMRX5aw5Ewyk--c82VyLoS81SVT3gkbM8d8DB7PxiqxGtSdFWAQE1VyTBQ-Oc2P5vx4kS2G-CfMhQn1IbYbbqgZvb1GBW-sv67kPObTZDFEaJXAbSbAJ-_yHIgFEqRv21JScG7fVjLIAG8fR_XcONLIVd1vVAur5kO7i5v46Jje9v5p9oAsNzMLYSnLybrTE3Nxrejj_5n6Q7I30Fj6LuDuEbnjqsdk92T4UP-EXAL8KEYu0tpT8-f35fKsg98QIvbTUaxSscIwBYA_xY8HLfxF-zQWjMOmzQ8F8KF9IH1IvWhxJH2OhnJFsdlI01LMjcFj13T1Rd0Bziq4yFllAa-Whm1lGAVA3j4l89nH7--P4qH5Q2xgbkWcaofl8gpvWKq9TRXjTHidC59bPk0V8C7uDNhrMDngcL3jwoBWJJmxiU6VYM_ITlVXbp9Qr4QxQF1NbkqelUkJL5k2NZw5C2RMZxEpNs9ZmqEyOjboOJejNyQuccElLrjsF1z-ish0K9mE6iC3kDkYQWkrCNwKuDqbRoRusCVB1_EZqMrV61aKHNyByEQRkecBc3-FwVRnrEwikvfIufXtyMPjOR69-FfBN2T364eZ_PLp9PNLci9suWM86Cuy063W7jVwtk4f9Mp4BfsqNoo |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+role+of+c%E2%80%90kit%E2%80%90positive+interstitial+cells+in+mediating+phasic+contractions+of+bladder+strips+from+streptozotocin%E2%80%90induced+diabetic+rats&rft.jtitle=BJU+international&rft.au=Vahabi%2C+Bahareh&rft.au=McKay%2C+Neil+G.&rft.au=Lawson%2C+Kim&rft.au=Sellers%2C+Donna+J.&rft.date=2011-05-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=1464-4096&rft.eissn=1464-410X&rft.volume=107&rft.issue=9&rft.spage=1480&rft.epage=1487&rft_id=info:doi/10.1111%2Fj.1464-410X.2010.09507.x&rft.externalDBID=10.1111%252Fj.1464-410X.2010.09507.x&rft.externalDocID=BJU9507 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1464-4096&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1464-4096&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1464-4096&client=summon |