Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil

• Published in: Environmental microbiology Vol. 5; no. 1; pp. 36 - 47
• Main Authors: Anderson, Ian C., Campbell, Colin D., Prosser, James I.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.01.2003
• Subjects: Bias; DNA Primers; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - genetics; Ecosystem; Fungi - classification; Fungi - genetics; Genetic Variation; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal, 18S - genetics; Sensitivity and Specificity; Sequence Analysis, DNA; Soil Microbiology

Summary Four fungal 18S rDNA and internal transcribed spacer (ITS) polymerase chain reaction (PCR) primer pairs were tested for their specificity towards target fungal DNA in soil DNA extracts, and their ability to assess the diversity of fungal communities in a natural grassland soil was compared....