Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth - Li, Zhongze; Kim, Minjae; Silva Nascimento, Jose Roberto; Legeret, Bertrand; Jorge, Gabriel Lemes; Bertrand, Marie; Beisson, Fred; Thelen, Jay J.; Li‐Beisson, Yonghua (Plant biotechnology journal, 2025)
Keywords: Accumulation; acetyl-CoA carboxylase; Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Algae; antioxidants; Biotechnology; bricks; Carbon; carbon metabolism; Carboxyltransferase; carboxyltransferase interactor; Cell growth; Chemical synthesis; Chlamydomonas; Chlamydomonas - genetics; Chlamydomonas - growth & development; Chlamydomonas - metabolism; Chlamydomonas reinhardtii - genetics; Chlamydomonas reinhardtii - metabolism; Chloroplasts; Cloning; CRISPR; CRISPR-Cas systems; de novo fatty acid synthesis; Down-regulation; Fatty acids; Fatty Acids - metabolism; Flowers & plants; Gene Knockout Techniques; Genetic engineering; Growth conditions; Investigations; lipid content; Lipids; microalgae; Mutants; Mutation; Oxidation; Peptides; peroxisome fatty acid β‐oxidation; phenotype; Phenotypes; Phosphorylation; Plant Oils - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; protein-protein interactions; Proteins; Proteomics; reaction kinetics; ROS; triacylglycerols; Triglycerides; Triglycerides - metabolism; two hybrid system techniques; Yeast; cell growth; peroxisome fatty acid β‐oxidation; de novo fatty acid synthesis; acetyl‐CoA carboxylase; carboxyltransferase interactor; ROS