Prevalence of Drug Resistance Mutations and Non-B Subtypes in Newly Diagnosed HIV-1 Patients in Denmark

• Published in: Scandinavian journal of infectious diseases Vol. 35; no. 11-12; pp. 800 - 807
• Main Authors: B. Jørgensen, Louise, B. Christensen, Marianne, Gerstoft, Jan, R. Mathiesen, Lars, Obel, Niels, Pedersen, Court, Nielsen, Henrik, Nielsen, Claus
• Format: Journal Article
• Language: English
• Published: Basingstoke Informa UK Ltd 01.12.2003; Taylor & Francis
• Subjects: Adolescent; Adult; Aged; Anti-HIV Agents - pharmacology; Anti-HIV Agents - therapeutic use; Biological and medical sciences; Blotting, Western; Child; Cohort Studies; Denmark - epidemiology; DNA Mutational Analysis; DNA, Viral - analysis; DNA, Viral - genetics; Drug Resistance, Multiple, Viral; Female; Fundamental and applied biological sciences. Psychology; HIV Infections - diagnosis; HIV Infections - drug therapy; HIV Infections - epidemiology; HIV Protease - genetics; HIV Reverse Transcriptase - genetics; HIV-1 - drug effects; HIV-1 - genetics; HIV-1 - isolation & purification; Human immunodeficiency virus 1; Human viral diseases; Humans; Infectious diseases; Male; Medical sciences; Microbial Sensitivity Tests; Microbiology; Middle Aged; Pharmacogenetics; Point Mutation - genetics; pol gene; Prevalence; Sampling Studies; Serologic Tests; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viral Load; zidovudine; Denmark; Europe; Immunopathology; Treatment resistance; Prevalence; Typing; Microbiology; AIDS; Epidemiology; Immune deficiency; Infection; Microbiological investigation; Viral disease; Diagnosis; Mutation; Subtype
• ISSN: 0036-5548; 1651-1980
• DOI: 10.1080/00365540310016916

The aim of this study was to monitor the prevalence of drug resistance mutations in newly diagnosed HIV-1 positive individuals in Denmark. In addition we assessed the prevalence of non-B subtypes based on phylogenetic analysis of the pol gene. Plasma samples from 104 newly diagnosed HIV-1 positive patients were obtained in the year 2000. The entire protease gene and 320 amino acids of the reverse transcriptase gene were genotyped. Sequences were obtained from 97 patients. No subjects displayed primary resistance mutations in the protease gene, whereas all carried 1 or more secondary mutations. Resistance mutations in the RT-gene associated with NRTI-resistance were found in 1 patient, who was infected with zidovudine resistant HIV-1 harbouring the M41L mutation in combination with T215S and L210S. The T215S mutation has been showed to be associated with reversion of zidovudine resistance. The T215S mutation was found in 1 additional patient. The subtype distribution was subtype B 59%, C 18%, A 8%, CRF02_AG 5%, CRF01_AE 4%, D 3% and G 2%. We found 2 patients (2%) with mutations associated with resistance in the RT-gene and none in the protease gene indicating a low prevalence of resistant HIV-1 in Denmark in the year 2000.