A nickase Cas9 gene-drive system promotes super-Mendelian inheritance in Drosophila

• Published in: Cell reports (Cambridge) Vol. 39; no. 8; p. 110843
• Main Authors: López Del Amo, Víctor, Juste, Sara Sanz, Gantz, Valentino M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.05.2022
• Subjects: CRISPR; Drosophila; gene drives; germline; homology-directed repair; nickase; CRISPR; germline; Drosophila; CP: Microbiology; CP: Molecular biology; gene drives; homology-directed repair; nickase

CRISPR-based gene-drives have been proposed for managing insect populations, including disease-transmitting mosquitoes, due to their ability to bias their inheritance toward super-Mendelian rates (>50%). Current technologies use a Cas9 that introduces DNA double-strand breaks into the opposing wi...