In Vitro Characterization of a Multifunctional Staphylokinase Variant with Reduced Reocclusion, Produced from Salt Inducible E. coli GJ1158

• Published in: BioMed research international Vol. 2013; no. 2013; pp. 1 - 12
• Main Authors: Kotra, Seetha Ram, Gadupudi, G. S., Kumar, Anmol, Pulicherla, K. K., Sambasiva Rao, K. R. S.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Publishing Corporation 01.01.2013; John Wiley & Sons, Inc
• Subjects: Cells, Cultured; Escherichia coli; Escherichia coli - physiology; Genetic Enhancement - methods; Genetic Variation; Humans; Metalloendopeptidases - genetics; Metalloendopeptidases - metabolism; Metalloendopeptidases - pharmacology; Methods; Phosphotransferases; Platelet Aggregation - drug effects; Platelet Aggregation Inhibitors - metabolism; Platelet Aggregation Inhibitors - pharmacology; Properties; Protein Engineering - methods; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - pharmacology; Recombinant proteins; Salts - metabolism; Thrombolytic therapy

The thrombolytic therapy with clinically approved drugs often ensues with recurrent thrombosis caused by thrombin-induced platelet aggregation from the clot debris. In order to minimize these problems, a staphylokinase (SAK)-based bacterial friendly multifunctional recombinant protein SRH (staphylok...