Subcellular Localization and Sequence of Sea Urchin Kinesin Heavy Chain: Evidence for Its Association with Membranes in the Mitotic Apparatus and Interphase Cytoplasm

• Published in: The Journal of cell biology Vol. 113; no. 4; pp. 817 - 833
• Main Authors: Wright, Brent D., Henson, John H., Wedaman, Karen P., Willy, Patricia J., Morand, Janice N., Scholey, Jonathan M.
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.05.1991; The Rockefeller University Press
• Subjects: Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Amino Acid Sequence; Animals; Antibodies; Antibodies, Monoclonal - immunology; biochemical analysis; Biological and medical sciences; Blotting, Western; Calsequestrin - metabolism; Cell Compartmentation; cell constituents; cell division; Cell physiology; Cells; Cleavage Stage, Ovum; Cloning, Molecular; Complementary DNA; cytology; Cytoplasm; Detergents; DNA - genetics; Echinoidea; Eggs; Embryos; Fundamental and applied biological sciences. Psychology; Interphase; Intracellular Membranes - metabolism; Kinesin; Marine; Microtubules - metabolism; mitosis; Mitotic spindle apparatus; Molecular and cellular biology; Molecular Sequence Data; Nocodazole - pharmacology; Peritoneal Cavity - cytology; Polymerase chain reaction; Restriction Mapping; Sea urchins; Sea Urchins - cytology; Sea Urchins - ultrastructure; Spindle Apparatus - metabolism; Spindle Apparatus - ultrastructure; Tubulin - metabolism; Echinodermata; heavy-Peptide chain; Plasma membrane; Molecular association; Mitotic apparatus; Echinoidea; Invertebrata; Localization; Sea urchin; Cytoplasm

Kinesin was previously immunolocalized to mitotic apparatuses (MAs) of early sea urchin blastomeres. Here we report evidence that this MA-associated motor protein is a conventional membrane-bound kinesin, rather than a kinesin-like protein. Our evidence includes the observation that the deduced amin...