All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human iPSC-Derived Motor Neuron Model of ALS

• Published in: Stem cell reports Vol. 10; no. 6; pp. 1991 - 2004
• Main Authors: Kiskinis, Evangelos, Kralj, Joel M., Zou, Peng, Weinstein, Eli N., Zhang, Hongkang, Tsioras, Konstantinos, Wiskow, Ole, Ortega, J. Alberto, Eggan, Kevin, Cohen, Adam E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 05.06.2018; Elsevier
• Subjects: Action Potentials; all-optical electrophysiology; ALS; Amyotrophic Lateral Sclerosis; Biomarkers; Electrophysiological Phenomena; Gene Editing; Gene Expression; Humans; Induced Pluripotent Stem Cells - cytology; iPSC; Molecular Imaging; motor neurons; Motor Neurons - cytology; Motor Neurons - physiology; Mutation; Resource; Superoxide Dismutase-1 - genetics; Superoxide Dismutase-1 - metabolism; motor neurons; ALS; all-optical electrophysiology; iPSC

Human induced pluripotent stem cell (iPSC)-derived neurons are an attractive substrate for modeling disease, yet the heterogeneity of these cultures presents a challenge for functional characterization by manual patch-clamp electrophysiology. Here, we describe an optimized all-optical electrophysiol...