Cholera toxin enhances Na + absorption across MCF10A human mammary epithelia

• Published in: American Journal of Physiology: Cell Physiology Vol. 306; no. 5; pp. C471 - C484
• Main Authors: Wang, Qian, Schultz, Bruce D.
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.03.2014
• Subjects: Absorption; Cell culture; Cell Line; Cell Membrane - drug effects; Cell Membrane - metabolism; Cell Membrane Permeability - drug effects; Cells; Cholera; Cholera Toxin - pharmacology; Endocrine system; Endosomal Sorting Complexes Required for Transport - metabolism; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Sodium Channel Blockers - pharmacology; Epithelial Sodium Channels - drug effects; Epithelial Sodium Channels - genetics; Epithelial Sodium Channels - metabolism; Female; Human subjects; Humans; Hydrocortisone - pharmacology; Ion Transport; Mammary Glands, Human - drug effects; Mammary Glands, Human - metabolism; Membrane Potentials; Nedd4 Ubiquitin Protein Ligases; Phosphorylation; Proteins; RNA, Messenger - metabolism; Sodium; Sodium - metabolism; Time Factors; Toxins; Ubiquitin-Protein Ligases - metabolism; Ubiquitination; amiloride; cholera toxin; Isc; ENaC; mammary gland; epithelial Na+ channel; short-circuit current

Cellular mechanisms to account for the low Na + concentration in human milk are poorly defined. MCF10A cells, which were derived from human mammary epithelium and grown on permeable supports, exhibit amiloride- and benzamil-sensitive short-circuit current ( I sc ; a sensitive indicator of net ion tr...