Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways

• Published in: Developmental cell Vol. 43; no. 6; pp. 744 - 762.e11
• Main Authors: Sigg, Monika Abedin, Menchen, Tabea, Lee, Chanjae, Johnson, Jeffery, Jungnickel, Melissa K., Choksi, Semil P., Garcia, Galo, Busengdal, Henriette, Dougherty, Gerard W., Pennekamp, Petra, Werner, Claudius, Rentzsch, Fabian, Florman, Harvey M., Krogan, Nevan, Wallingford, John B., Omran, Heymut, Reiter, Jeremy F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.12.2017
• Subjects: Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Animals; Calmodulin-Binding Proteins - genetics; Calmodulin-Binding Proteins - metabolism; Cell Culture Techniques; Choanoflagellata - metabolism; choanoflagellate; Cilia - genetics; Cilia - metabolism; ciliopathy; cilium; GPCR; Hedgehog Proteins - metabolism; Hedgehog signaling; Humans; left-right axis patterning; Mice; Mutation; Organelles - metabolism; Phylogeny; proteomics; Proteomics - methods; Receptors, G-Protein-Coupled - metabolism; sea anemone; Sea Anemones - metabolism; sea urchin; Sea Urchins - metabolism; Signal Transduction - genetics; Signal Transduction - physiology; Transient Receptor Potential Channels - metabolism; TRP channel; Xenopus laevis - metabolism; Zebrafish - metabolism; cilium; TRP channel; left-right axis patterning; proteomics; Hedgehog signaling; GPCR; sea urchin; sea anemone; choanoflagellate; ciliopathy

Cilia are organelles specialized for movement and signaling. To infer when during evolution signaling pathways became associated with cilia, we characterized the proteomes of cilia from sea urchins, sea anemones, and choanoflagellates. We identified 437 high-confidence ciliary candidate proteins con...