Characterization of adipose‐derived stromal/stem cell spheroids versus single‐cell suspension in cell survival and arrest of osteoarthritis progression
The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and arrests the progression of surgically induced osteoarthritis (OA) in a rat model. We also tested the optimal conditions for spheroid production from A...
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Published in | Journal of biomedical materials research. Part A Vol. 109; no. 6; pp. 869 - 878 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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Hoboken, USA
John Wiley & Sons, Inc
01.06.2021
Wiley Subscription Services, Inc |
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Abstract | The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and arrests the progression of surgically induced osteoarthritis (OA) in a rat model. We also tested the optimal conditions for spheroid production from ASCs using microwell methods. The formation of ASC spheroids was optimized at a well diameter of 600 μm under cell concentrations of 106cell/ml. When ASC spheroids cultured in 3D were compared with ASCs cultured in 2D monolayer, the cell survival and chondrogenic potential were enhanced while the apoptosis was reduced in ASC spheroids compared with ASCs in 2D monolayer culture. In vivo tracking of fluorescently labeled ASCs in the knee joints of rats with surgically induced OA showed longer fluorescent activity at a higher intensity in ASC spheroids than in ASC single‐cell suspension. When OA‐induced rats treated with ASC injection were sacrificed after 8 weeks, the OARSI score was enhanced in both ASC single‐cell suspension and ASC spheroids compared with negative control, spheroid treatment resulting in a better score than single‐cell treatment. However, injected cells were not detectable from the joints. These finding altogether suggests that ASC spheroids have better in vitro and in vivo survival and chondrogenic potential and exert greater regenerative effects for articular cartilage and arrest the progression of surgically induced OA better than ASCs in single‐cell suspension by the paracrine mode of action. The study findings support the notion of developing cell therapeutics to treat OA based on ASC spheroid forms. |
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AbstractList | Abstract
The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and arrests the progression of surgically induced osteoarthritis (OA) in a rat model. We also tested the optimal conditions for spheroid production from ASCs using microwell methods. The formation of ASC spheroids was optimized at a well diameter of 600 μm under cell concentrations of 10
6
cell/ml. When ASC spheroids cultured in 3D were compared with ASCs cultured in 2D monolayer, the cell survival and chondrogenic potential were enhanced while the apoptosis was reduced in ASC spheroids compared with ASCs in 2D monolayer culture. In vivo tracking of fluorescently labeled ASCs in the knee joints of rats with surgically induced OA showed longer fluorescent activity at a higher intensity in ASC spheroids than in ASC single‐cell suspension. When OA‐induced rats treated with ASC injection were sacrificed after 8 weeks, the OARSI score was enhanced in both ASC single‐cell suspension and ASC spheroids compared with negative control, spheroid treatment resulting in a better score than single‐cell treatment. However, injected cells were not detectable from the joints. These finding altogether suggests that ASC spheroids have better in vitro and in vivo survival and chondrogenic potential and exert greater regenerative effects for articular cartilage and arrest the progression of surgically induced OA better than ASCs in single‐cell suspension by the paracrine mode of action. The study findings support the notion of developing cell therapeutics to treat OA based on ASC spheroid forms. The current study evaluated the hypothesis that the administration of spheroidal adipose-derived stromal/stem cells (ASCs) promotes cell survival and arrests the progression of surgically induced osteoarthritis (OA) in a rat model. We also tested the optimal conditions for spheroid production from ASCs using microwell methods. The formation of ASC spheroids was optimized at a well diameter of 600 μm under cell concentrations of 10 cell/ml. When ASC spheroids cultured in 3D were compared with ASCs cultured in 2D monolayer, the cell survival and chondrogenic potential were enhanced while the apoptosis was reduced in ASC spheroids compared with ASCs in 2D monolayer culture. In vivo tracking of fluorescently labeled ASCs in the knee joints of rats with surgically induced OA showed longer fluorescent activity at a higher intensity in ASC spheroids than in ASC single-cell suspension. When OA-induced rats treated with ASC injection were sacrificed after 8 weeks, the OARSI score was enhanced in both ASC single-cell suspension and ASC spheroids compared with negative control, spheroid treatment resulting in a better score than single-cell treatment. However, injected cells were not detectable from the joints. These finding altogether suggests that ASC spheroids have better in vitro and in vivo survival and chondrogenic potential and exert greater regenerative effects for articular cartilage and arrest the progression of surgically induced OA better than ASCs in single-cell suspension by the paracrine mode of action. The study findings support the notion of developing cell therapeutics to treat OA based on ASC spheroid forms. The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and arrests the progression of surgically induced osteoarthritis (OA) in a rat model. We also tested the optimal conditions for spheroid production from ASCs using microwell methods. The formation of ASC spheroids was optimized at a well diameter of 600 μm under cell concentrations of 106cell/ml. When ASC spheroids cultured in 3D were compared with ASCs cultured in 2D monolayer, the cell survival and chondrogenic potential were enhanced while the apoptosis was reduced in ASC spheroids compared with ASCs in 2D monolayer culture. In vivo tracking of fluorescently labeled ASCs in the knee joints of rats with surgically induced OA showed longer fluorescent activity at a higher intensity in ASC spheroids than in ASC single‐cell suspension. When OA‐induced rats treated with ASC injection were sacrificed after 8 weeks, the OARSI score was enhanced in both ASC single‐cell suspension and ASC spheroids compared with negative control, spheroid treatment resulting in a better score than single‐cell treatment. However, injected cells were not detectable from the joints. These finding altogether suggests that ASC spheroids have better in vitro and in vivo survival and chondrogenic potential and exert greater regenerative effects for articular cartilage and arrest the progression of surgically induced OA better than ASCs in single‐cell suspension by the paracrine mode of action. The study findings support the notion of developing cell therapeutics to treat OA based on ASC spheroid forms. |
Author | Im, Gun‐Il Park, Jeong‐Won Kim, Juyoung Ko, Ji‐Yun |
Author_xml | – sequence: 1 givenname: Ji‐Yun surname: Ko fullname: Ko, Ji‐Yun organization: Dongguk University – sequence: 2 givenname: Jeong‐Won surname: Park fullname: Park, Jeong‐Won organization: Dongguk University – sequence: 3 givenname: Juyoung surname: Kim fullname: Kim, Juyoung organization: Dongguk University – sequence: 4 givenname: Gun‐Il surname: Im fullname: Im, Gun‐Il email: gunil@duih.org organization: Dongguk University Ilsan Hospital |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32776432$$D View this record in MEDLINE/PubMed |
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Keywords | articular cartilage chondrogenesis osteoarthritis adipose-derived stromal/stem cells spheroid |
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Snippet | The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and arrests... The current study evaluated the hypothesis that the administration of spheroidal adipose-derived stromal/stem cells (ASCs) promotes cell survival and arrests... Abstract The current study evaluated the hypothesis that the administration of spheroidal adipose‐derived stromal/stem cells (ASCs) promotes cell survival and... |
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SubjectTerms | Adipose Tissue - cytology adipose‐derived stromal/stem cells Adult Aged Animals Apoptosis Arthritis articular cartilage Biomedical materials Cartilage Cartilage (articular) Cartilage diseases Cell culture Cell Survival Cell- and Tissue-Based Therapy - methods Cells, Cultured Chondrogenesis Disease Progression Female Fluorescence Hindlimb Humans Joints (anatomy) Joints - pathology Male Middle Aged Mode of action Monolayers Osteoarthritis Osteoarthritis - pathology Osteoarthritis - therapy Paracrine signalling Rats Rats, Sprague-Dawley Regeneration spheroid Spheroids Spheroids, Cellular Stem cell transplantation Stem Cell Transplantation - methods Stem Cells Survival Suspensions |
Title | Characterization of adipose‐derived stromal/stem cell spheroids versus single‐cell suspension in cell survival and arrest of osteoarthritis progression |
URI | https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fjbm.a.37078 https://www.ncbi.nlm.nih.gov/pubmed/32776432 https://www.proquest.com/docview/2509218220/abstract/ https://search.proquest.com/docview/2432857546 |
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