Tumors Associated With Oncogenic Osteomalacia Express Genes Important in Bone and Mineral Metabolism

• Published in: Journal of bone and mineral research Vol. 17; no. 6; pp. 1102 - 1110
• Main Authors: De Beur, Suzanne M. Jan, Finnegan, Richard B., Vassiliadis, John, Cook, Brian, Barberio, Dana, Estes, Scott, Manavalan, Partha, Petroziello, Joseph, Madden, Stephen L., Cho, Justin Y., Kumar, Rajiv, Levine, Michael A., Schiavi, Susan C.
• Format: Journal Article
• Language: English
• Published: Washington, DC John Wiley and Sons and The American Society for Bone and Mineral Research (ASBMR) 01.06.2002; American Society for Bone and Mineral Research
• Subjects: Base Sequence; Biological and medical sciences; Bone and Bones - metabolism; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Calcitriol - metabolism; Calcium - metabolism; Diseases of the osteoarticular system; DNA Primers; gene expression; Gene Expression Profiling; Humans; hypophosphatemia; Medical sciences; mesenchymal tumors; mineral metabolism; osteomalacia; Osteomalacia - genetics; Osteomalacia - metabolism; Parathyroid Hormone - metabolism; Phosphorus - metabolism; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; Tumors of striated muscle and skeleton; Human; Hydroelectrolytic balance disorder; Diseases of the osteoarticular system; Nutrition disorder; Mesenchyma; Vitamin deficiency; Bone disease; Gene expression; Metabolism; Density; Inorganic element; Osteoarticular system; Metabolic disorder; Hypophosphatemia; Tumor; Bone; Osteomalacia; Tumor cell; Nutritional status
• DOI: 10.1359/jbmr.2002.17.6.1102
• ISSN: 0884-0431

Oncogenic osteomalacia (OOM) is associated with primitive mesenchymal tumors that secrete phosphaturic factors resulting in low serum concentrations of phosphate and calcitriol, phosphaturia, and defective bone mineralization. To identify overexpressed genes in these tumors, we compared gene expression profiles of tumors resected from patients with OOM and histologically similar control tumors using serial analysis of gene expression (SAGE). Three hundred and sixty‐four genes were expressed at least twofold greater in OOM tumors compared with control tumors. A subset of 67 highly expressed genes underwent validation with an extended set of OOM and control tumors using array analysis or reverse‐transcription polymerase chain reaction (RT‐PCR). Ten of these validated genes were consistently overexpressed in all OOM tumors relative to control tumors. Strikingly, genes with roles in bone matrix formation, mineral ion transport, and bone mineralization were highly expressed in the OOM tumors.