A Novel DNA Synthesis Platform Design with High-Throughput Paralleled Addressability and High-Density Static Droplet Confinement

• Published in: Biosensors (Basel) Vol. 14; no. 4; p. 177
• Main Authors: Yang, Shijia, Wang, Dayin, Zhao, Zequan, Wang, Ning, Yu, Meng, Zhang, Kaihuan, Luo, Yuan, Zhao, Jianlong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: Analysis; Arrays; Biosensing Techniques; Contact angle; Data storage; Deoxyribonucleic acid; Design; DNA; DNA biosynthesis; DNA data storage; DNA synthesis; DRAM; Droplets; Dynamic random access memory; Electric potential; Electrochemistry; Electrodes; Equipment and supplies; Fluorescence; High density; High-throughput screening (Biochemical assaying); Integrated circuits; Methods; Microfluidics; Power consumption; Semiconductor chips; Silicon wafers; Simulation; Species diffusion; static droplet; Synthesis; Theoretical analysis; Voltage; China; DNA synthesis; DRAM; microfluidics; DNA data storage; static droplet
• DOI: 10.3390/bios14040177
• ISSN: 2079-6374

Using DNA as the next-generation medium for data storage offers unparalleled advantages in terms of data density, storage duration, and power consumption as compared to existing data storage technologies. To meet the high-speed data writing requirements in DNA data storage, this paper proposes a novel design for an ultra-high-density and high-throughput DNA synthesis platform. The presented design mainly leverages two functional modules: a dynamic random-access memory (DRAM)-like integrated circuit (IC) responsible for electrode addressing and voltage supply, and the static droplet array (SDA)-based microfluidic structure to eliminate any reaction species diffusion concern in electrochemical DNA synthesis. Through theoretical analysis and simulation studies, we validate the effective addressing of 10 million electrodes and stable, adjustable voltage supply by the integrated circuit. We also demonstrate a reaction unit size down to 3.16 × 3.16 μm2, equivalent to 10 million/cm2, that can rapidly and stably generate static droplets at each site, effectively constraining proton diffusion. Finally, we conducted a synthesis cycle experiment by incorporating fluorescent beacons on a microfabricated electrode array to examine the feasibility of our design.