Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET

• Published in: Frontiers in plant science Vol. 12; p. 747886
• Main Authors: Kalendar, Ruslan, Baidyussen, Akmaral, Serikbay, Dauren, Zotova, Lyudmila, Khassanova, Gulmira, Kuzbakova, Marzhan, Jatayev, Satyvaldy, Hu, Yin-Gang, Schramm, Carly, Anderson, Peter A, Jenkins, Colin L D, Soole, Kathleen L, Shavrukov, Yuri
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 10.01.2022
• Subjects: allele-specific primers; fluorescence and quenching; FRET-based method; genotyping; Plant Science; qPCR and plate reader instruments; single nucleotide polymorphism (SNP); fluorescence and quenching; single nucleotide polymorphism (SNP); FRET-based method; genotyping; allele-specific primers; qPCR and plate reader instruments; universal probes

The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor an...