Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET

The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor an...

Full description

Saved in:
Bibliographic Details
Published inFrontiers in plant science Vol. 12; p. 747886
Main Authors Kalendar, Ruslan, Baidyussen, Akmaral, Serikbay, Dauren, Zotova, Lyudmila, Khassanova, Gulmira, Kuzbakova, Marzhan, Jatayev, Satyvaldy, Hu, Yin-Gang, Schramm, Carly, Anderson, Peter A, Jenkins, Colin L D, Soole, Kathleen L, Shavrukov, Yuri
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 10.01.2022
Subjects
Online AccessGet full text

Cover

Loading…
Abstract The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor and Kompetitive allele specific PCR (KASP), as well as others employing common universal probes (UPs) for SNP analyses. In the proposed ASQ method, the fluorophores and quencher are located in separate complementary oligonucleotides. The ASQ method is based on the simultaneous presence in PCR of the following two components: an allele-specific mixture (allele-specific and common primers) and a template-independent detector mixture that contains two or more (up to four) universal probes (UP-1 to 4) and a single universal quencher oligonucleotide (Uni-Q). The SNP site is positioned preferably at a penultimate base in each allele-specific primer, which increases the reaction specificity and allele discrimination. The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments. Importantly, this ASQ method is estimated as two- to ten-fold cheaper than Amplifluor and KASP, and much cheaper than all those methods that rely on dual-labeled probes without universal components, like TaqMan and Molecular Beacons. Results for SNP genotyping in the barley genes and , in which stress-associated proteins are controlled, are presented as proven and validated examples. This method is suitable for bi-allelic uniplex reactions but it can potentially be used for 3- or 4-allelic variants or different SNPs in a multiplex format in a range of applications including medical, forensic, or others involving SNP genotyping.
AbstractList The proposed method is a modified and improved version of the existing “Allele-specific q-PCR” (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor and Kompetitive allele specific PCR (KASP), as well as others employing common universal probes (UPs) for SNP analyses. In the proposed ASQ method, the fluorophores and quencher are located in separate complementary oligonucleotides. The ASQ method is based on the simultaneous presence in PCR of the following two components: an allele-specific mixture (allele-specific and common primers) and a template-independent detector mixture that contains two or more (up to four) universal probes (UP-1 to 4) and a single universal quencher oligonucleotide (Uni-Q). The SNP site is positioned preferably at a penultimate base in each allele-specific primer, which increases the reaction specificity and allele discrimination. The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments. Importantly, this ASQ method is estimated as two- to ten-fold cheaper than Amplifluor and KASP, and much cheaper than all those methods that rely on dual-labeled probes without universal components, like TaqMan and Molecular Beacons. Results for SNP genotyping in the barley genes HvSAP16 and HvSAP8 , in which stress-associated proteins are controlled, are presented as proven and validated examples. This method is suitable for bi-allelic uniplex reactions but it can potentially be used for 3- or 4-allelic variants or different SNPs in a multiplex format in a range of applications including medical, forensic, or others involving SNP genotyping.
The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor and Kompetitive allele specific PCR (KASP), as well as others employing common universal probes (UPs) for SNP analyses. In the proposed ASQ method, the fluorophores and quencher are located in separate complementary oligonucleotides. The ASQ method is based on the simultaneous presence in PCR of the following two components: an allele-specific mixture (allele-specific and common primers) and a template-independent detector mixture that contains two or more (up to four) universal probes (UP-1 to 4) and a single universal quencher oligonucleotide (Uni-Q). The SNP site is positioned preferably at a penultimate base in each allele-specific primer, which increases the reaction specificity and allele discrimination. The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments. Importantly, this ASQ method is estimated as two- to ten-fold cheaper than Amplifluor and KASP, and much cheaper than all those methods that rely on dual-labeled probes without universal components, like TaqMan and Molecular Beacons. Results for SNP genotyping in the barley genes and , in which stress-associated proteins are controlled, are presented as proven and validated examples. This method is suitable for bi-allelic uniplex reactions but it can potentially be used for 3- or 4-allelic variants or different SNPs in a multiplex format in a range of applications including medical, forensic, or others involving SNP genotyping.
The proposed method is a modified and improved version of the existing “Allele-specific q-PCR” (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor and Kompetitive allele specific PCR (KASP), as well as others employing common universal probes (UPs) for SNP analyses. In the proposed ASQ method, the fluorophores and quencher are located in separate complementary oligonucleotides. The ASQ method is based on the simultaneous presence in PCR of the following two components: an allele-specific mixture (allele-specific and common primers) and a template-independent detector mixture that contains two or more (up to four) universal probes (UP-1 to 4) and a single universal quencher oligonucleotide (Uni-Q). The SNP site is positioned preferably at a penultimate base in each allele-specific primer, which increases the reaction specificity and allele discrimination. The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments. Importantly, this ASQ method is estimated as two- to ten-fold cheaper than Amplifluor and KASP, and much cheaper than all those methods that rely on dual-labeled probes without universal components, like TaqMan and Molecular Beacons. Results for SNP genotyping in the barley genes HvSAP16 and HvSAP8, in which stress-associated proteins are controlled, are presented as proven and validated examples. This method is suitable for bi-allelic uniplex reactions but it can potentially be used for 3- or 4-allelic variants or different SNPs in a multiplex format in a range of applications including medical, forensic, or others involving SNP genotyping.
Author Khassanova, Gulmira
Anderson, Peter A
Zotova, Lyudmila
Kuzbakova, Marzhan
Serikbay, Dauren
Shavrukov, Yuri
Jatayev, Satyvaldy
Baidyussen, Akmaral
Hu, Yin-Gang
Jenkins, Colin L D
Kalendar, Ruslan
Soole, Kathleen L
Schramm, Carly
AuthorAffiliation 2 Institute of Biotechnology HiLIFE, University of Helsinki , Helsinki , Finland
5 College of Science and Engineering, Biological Sciences, Flinders University , Adelaide, SA , Australia
4 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University , Yangling , China
1 National Laboratory Astana, Nazarbayev University , Nur-Sultan , Kazakhstan
3 Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University , Nur-Sultan , Kazakhstan
AuthorAffiliation_xml – name: 4 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University , Yangling , China
– name: 5 College of Science and Engineering, Biological Sciences, Flinders University , Adelaide, SA , Australia
– name: 1 National Laboratory Astana, Nazarbayev University , Nur-Sultan , Kazakhstan
– name: 2 Institute of Biotechnology HiLIFE, University of Helsinki , Helsinki , Finland
– name: 3 Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University , Nur-Sultan , Kazakhstan
Author_xml – sequence: 1
  givenname: Ruslan
  surname: Kalendar
  fullname: Kalendar, Ruslan
  organization: Institute of Biotechnology HiLIFE, University of Helsinki, Helsinki, Finland
– sequence: 2
  givenname: Akmaral
  surname: Baidyussen
  fullname: Baidyussen, Akmaral
  organization: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
– sequence: 3
  givenname: Dauren
  surname: Serikbay
  fullname: Serikbay, Dauren
  organization: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China
– sequence: 4
  givenname: Lyudmila
  surname: Zotova
  fullname: Zotova, Lyudmila
  organization: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
– sequence: 5
  givenname: Gulmira
  surname: Khassanova
  fullname: Khassanova, Gulmira
  organization: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
– sequence: 6
  givenname: Marzhan
  surname: Kuzbakova
  fullname: Kuzbakova, Marzhan
  organization: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
– sequence: 7
  givenname: Satyvaldy
  surname: Jatayev
  fullname: Jatayev, Satyvaldy
  organization: Faculty of Agronomy, S. Seifullin Kazakh AgroTechnical University, Nur-Sultan, Kazakhstan
– sequence: 8
  givenname: Yin-Gang
  surname: Hu
  fullname: Hu, Yin-Gang
  organization: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China
– sequence: 9
  givenname: Carly
  surname: Schramm
  fullname: Schramm, Carly
  organization: College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia
– sequence: 10
  givenname: Peter A
  surname: Anderson
  fullname: Anderson, Peter A
  organization: College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia
– sequence: 11
  givenname: Colin L D
  surname: Jenkins
  fullname: Jenkins, Colin L D
  organization: College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia
– sequence: 12
  givenname: Kathleen L
  surname: Soole
  fullname: Soole, Kathleen L
  organization: College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia
– sequence: 13
  givenname: Yuri
  surname: Shavrukov
  fullname: Shavrukov, Yuri
  organization: College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA, Australia
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35082803$$D View this record in MEDLINE/PubMed
BookMark eNpVkUtPJCEUhYlx4mvcz8pUXLmpHigoHhsT7WhrojNGnWR2hKIubRm6KKHaxH8vbatRNpDLPece-HbRZh96QOgXwRNKpfrtBp8mFa7IRDAhJd9AO4RzVjJe_d_8ct5G-yk94rxqjJUSW2ib1lhWEtMdNLsObec6aIvDE-_BQ3k3gM0VWzzdTG8Pi2sYH0JbuBCLuz83xQz6ML4MXT8vTk3KstAX57dn9z_RD2d8gv33fQ_9Oz-7n16UV39nl9OTq9LmKGPpLBUs53DGEMcccEcY45xb64xirmECWyWpA6wk5jVw2SgLSvBacdVIQvfQ5dq3DeZRD7FbmPiig-n0WyHEuTZx7KwHTamtoW6AqpoxCsI0ijrRcisIqZS02et47TUsmwW0FvoxGv_N9PtN3z3oeXjWUsj846swR-8GMTwtIY160SUL3psewjLpileUVnV-cW7F61YbQ0oR3OcYgvUKp17h1Cuceo0zSw6-xvsUfMCjr1kmm9Y
CitedBy_id crossref_primary_10_3389_fbioe_2023_1279473
crossref_primary_10_1016_j_bios_2023_115799
crossref_primary_10_3389_fpls_2023_1174339
crossref_primary_10_3390_ijms222212156
crossref_primary_10_3389_fpls_2024_1354413
crossref_primary_10_1002_dta_3438
crossref_primary_10_3389_fmolb_2022_773956
Cites_doi 10.1021/bi962590c
10.1007/s11816-017-0432-0
10.1186/1746-4811-6-12
10.1016/j.ab.2004.03.035
10.1093/nar/21.22.5137
10.1038/nmeth0508-447
10.3389/fpls.2018.01441
10.1093/nar/29.20.e96
10.1101/gr.157901
10.1016/j.mcp.2014.12.003
10.1039/d0ay01961e
10.1007/s00122-020-03609-w
10.1093/clinchem/47.3.486
10.1186/s12896-019-0549-z
10.1186/s12870-017-1197-x
10.5772/intechopen.91886
10.1146/annurev.bioeng.9.060906.152037
10.1073/pnas.95.4.1460
10.1111/pbr.12686
10.1186/s13007-018-0295-6
10.2144/01315rv02
10.1517/14622416.1.2.219
10.3389/fpls.2018.00104
10.2353/jmoldx.2010.090035
10.7717/peerj.10581
10.1038/sj.tpj.6500167
10.19103/AS.2019.0051.16
10.1007/978-1-0716-1799-1_16
10.1101/pdb.top62
10.1007/s00216-009-3418-0
10.1016/j.mimet.2009.03.001
10.1038/hortres.2014.65
10.1093/nar/28.19.3752
10.1007/s00122-016-2743-x
10.5772/52583
10.1186/1472-6750-11-80
10.1016/j.ejbt.2014.02.003
10.1007/s10142-009-0153-8
10.1016/j.ygeno.2017.05.005
10.1093/nar/25.12.2516
10.1186/1746-4811-8-34
10.1111/j.1364-3703.2006.00345.x
10.1186/1746-4811-5-1
10.1007/s13258-017-0554-3
10.1016/j.indcrop.2020.112983
10.3389/fgene.2019.00063
10.1002/9780470958964.ch8
10.1134/S2079059713040114
10.1186/s12864-019-5533-4
10.1002/ps.6278
10.1007/978-1-0716-1201-9_6
10.9787/PBB.2014.2.3.195
10.1016/j.molp.2017.06.008
10.1146/annurev-genom-082908-150116
10.1007/s42976-020-00057-6
10.1016/B978-0-85709-801-6.00009-5
10.1186/s12870-020-02332-4
10.1371/journal.pone.0002882
10.3390/ijms21218296
10.1007/s11032-019-1091-3
10.1007/978-1-0716-0997-2_3
10.1038/s41598-021-83908-4
10.1007/s00122-006-0213-6
10.1016/j.ejbt.2018.08.001
10.1021/jf063725g
10.1016/j.scienta.2018.09.053
10.1371/journal.pone.0217222
10.1007/s00122-016-2838-4
10.3389/fpls.2016.01736
10.1089/hum.2016.011
10.3389/fgene.2019.00040
10.1385/1-59745-069-3:215
10.1155/2012/831460
10.1186/s12870-020-02331-5
ContentType Journal Article
Copyright Copyright © 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov.
Copyright © 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov. 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov
Copyright_xml – notice: Copyright © 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov.
– notice: Copyright © 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov. 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov
DBID NPM
AAYXX
CITATION
7X8
5PM
DOA
DOI 10.3389/fpls.2021.747886
DatabaseName PubMed
CrossRef
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle PubMed
CrossRef
MEDLINE - Academic
DatabaseTitleList
PubMed

CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Botany
EISSN 1664-462X
EndPage 747886
ExternalDocumentID oai_doaj_org_article_33c5e5be395443e7ab93f7d6c711298c
10_3389_fpls_2021_747886
35082803
Genre Journal Article
GroupedDBID 5VS
9T4
AAFWJ
AAKDD
ACGFO
ACGFS
ACXDI
ADBBV
ADRAZ
AENEX
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BCNDV
EBD
ECGQY
GROUPED_DOAJ
GX1
HYE
IAO
IEA
IGS
IPNFZ
ISR
KQ8
M48
M~E
NPM
OK1
PGMZT
RIG
RNS
RPM
AAYXX
CITATION
7X8
5PM
AFPKN
ID FETCH-LOGICAL-c462t-fc374050faa1f4fe6f144666ccfa94fb470c983fe098065e68b9ce9765969b813
IEDL.DBID RPM
ISSN 1664-462X
IngestDate Tue Oct 22 15:12:50 EDT 2024
Tue Sep 17 21:21:00 EDT 2024
Thu Oct 24 23:51:54 EDT 2024
Thu Nov 21 22:41:10 EST 2024
Wed Oct 16 00:42:40 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Keywords fluorescence and quenching
single nucleotide polymorphism (SNP)
FRET-based method
genotyping
allele-specific primers
qPCR and plate reader instruments
universal probes
Language English
License Copyright © 2022 Kalendar, Baidyussen, Serikbay, Zotova, Khassanova, Kuzbakova, Jatayev, Hu, Schramm, Anderson, Jenkins, Soole and Shavrukov.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c462t-fc374050faa1f4fe6f144666ccfa94fb470c983fe098065e68b9ce9765969b813
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Reviewed by: Alexandr Muterko, Institute of Cytology and Genetics, Russian Academy of Sciences (RAS), Russia; Mark Owen Winfield, University of Bristol, United Kingdom
Edited by: Roger Deal, Emory University, United States
These authors have contributed equally to this work and share first authorship
This article was submitted to Technical Advances in Plant Science, a section of the journal Frontiers in Plant Science
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784781/
PMID 35082803
PQID 2623325740
PQPubID 23479
PageCount 1
ParticipantIDs doaj_primary_oai_doaj_org_article_33c5e5be395443e7ab93f7d6c711298c
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8784781
proquest_miscellaneous_2623325740
crossref_primary_10_3389_fpls_2021_747886
pubmed_primary_35082803
PublicationCentury 2000
PublicationDate 2022-01-10
PublicationDateYYYYMMDD 2022-01-10
PublicationDate_xml – month: 01
  year: 2022
  text: 2022-01-10
  day: 10
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
PublicationTitle Frontiers in plant science
PublicationTitleAlternate Front Plant Sci
PublicationYear 2022
Publisher Frontiers Media S.A
Publisher_xml – name: Frontiers Media S.A
References Rasheed (B51) 2016; 129
B21
Gašparič (B14) 2010; 396
Ke (B29) 1993; 21
Khassanova (B30) 2019; 10
Ayalew (B2) 2019; 14
Baidyussen (B3) 2020; 20
Jiang (B22) 2013
Kalendar (B25) 2021
Wu (B69) 2009; 77
Allawi (B1) 1997; 36
Kim (B32) 2007; 9
Bui (B6) 2009; 5
Nazarenko (B46) 1997; 25
Wang (B67) 2015; 2
Weck (B68) 2021; 11
Fuhrman (B13) 2008; 3
Peatman (B47) 2011
Massa (B43) 2021; 9
Jawhari (B20) 2015; 29
Shavrukov (B59) 2010; 10
Schena (B57) 2006; 7
Perkel (B48) 2008; 5
Kumar (B34) 2012; 2012
Lu (B41) 2020; 40
Li (B37) 2019; 138
Choi (B8) 2017; 39
Didenko (B9) 2001; 31
Rasheed (B50) 2017; 10
Kalendar (B24) 2022
Stadhouders (B62) 2010; 12
Hirotsu (B18) 2010; 6
Lee (B36) 2016; 27
Löfström (B39) 2015
Edenberg (B11) 2009; 4
Wu (B70) 2020; 133
Zotova (B75) 2019; 10
Myakishev (B45) 2001; 11
Giancola (B15) 2006; 112
Ragoussis (B49) 2009; 10
Hardinge (B17) 2019; 19
Kaur (B27); 12
B42
Solinas (B61) 2001; 29
Shavrukov (B60) 2016; 7
La Paz (B35) 2007; 55
Long (B40) 2017; 130
Chen (B7) 2003; 3
Udoh (B66) 2020
Han (B16) 2017; 11
Kadirvel (B23) 2020; 158
Zhu (B73) 2021
Rickert (B52) 2004; 330
Morgil (B44) 2020
You (B71) 2018; 9
Zhao (B72) 2019; 20
Zlotina (B74) 2013; 3
Zotova (B76) 2018; 9
Jatayev (B19) 2017; 17
Kreuzer (B33) 2001; 47
Brusa (B5) 2021; 77
Schramm (B58) 2019
Fors (B12) 2000; 1
Thomson (B65) 2014; 2
Rosas (B53) 2014; 17
Khripin (B31) 2006
SantaLucia (B56) 1998; 95
Liu (B38) 2012; 8
Thelwell (B64) 2000; 28
Sweetman (B63) 2020; 20
Kalendar (B26) 2017; 109
Broccanello (B4) 2018; 14
Dobosy (B10) 2011; 11
Kaur (B28); 48
Zotova (B77) 2020; 21
Ryu (B54) 2019; 244
Ryu (B55) 2018; 35
References_xml – volume: 36
  start-page: 10581
  year: 1997
  ident: B1
  article-title: Thermodynamics and NMR of internal G-T mismatches in DNA
  publication-title: Biochemistry
  doi: 10.1021/bi962590c
  contributor:
    fullname: Allawi
– volume: 11
  start-page: 127
  year: 2017
  ident: B16
  article-title: Molecular marker analysis of Cynanchum wilfordii and C. auriculatum using the simple ARMS-PCR method with mismatched primers
  publication-title: Plant Biotechnol. Rep.
  doi: 10.1007/s11816-017-0432-0
  contributor:
    fullname: Han
– volume: 6
  start-page: 12
  year: 2010
  ident: B18
  article-title: Protocol: A simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species
  publication-title: Plant Methods
  doi: 10.1186/1746-4811-6-12
  contributor:
    fullname: Hirotsu
– volume: 330
  start-page: 288
  year: 2004
  ident: B52
  article-title: Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan
  publication-title: Anal Biochem
  doi: 10.1016/j.ab.2004.03.035
  contributor:
    fullname: Rickert
– volume: 21
  start-page: 5137
  year: 1993
  ident: B29
  article-title: Influence of nearest neighbor sequence on the stability of base pair mismatches in long DNA: determination by temperature-gradient gel electrophoresis
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/21.22.5137
  contributor:
    fullname: Ke
– volume: 5
  start-page: 447
  year: 2008
  ident: B48
  article-title: SNP genotyping: six technologies that keyed a revolution
  publication-title: Nat. Methods
  doi: 10.1038/nmeth0508-447
  contributor:
    fullname: Perkel
– volume: 9
  start-page: 1441
  year: 2018
  ident: B76
  article-title: Genes encoding transcription factors TaDREB5 and TaNFYC-A7 are differentially expressed in leaves of bread wheat in response to drought, dehydration and ABA
  publication-title: Front. Plant Sci
  doi: 10.3389/fpls.2018.01441
  contributor:
    fullname: Zotova
– volume: 29
  start-page: e96
  year: 2001
  ident: B61
  article-title: Duplex Scorpion primers in SNP analysis and FRET applications
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/29.20.e96
  contributor:
    fullname: Solinas
– volume: 11
  start-page: 163
  year: 2001
  ident: B45
  article-title: High-throughput SNP genotyping by allele-specific PCR with universal energy-transfer-labeled primers
  publication-title: Genome Res.
  doi: 10.1101/gr.157901
  contributor:
    fullname: Myakishev
– volume: 29
  start-page: 63
  year: 2015
  ident: B20
  article-title: Specific PCR and real-time PCR assays for detection and quantitation of “Candidatus Phytoplasma phoenicium.”
  publication-title: Mol. Cell. Probes
  doi: 10.1016/j.mcp.2014.12.003
  contributor:
    fullname: Jawhari
– volume: 12
  start-page: 5532
  ident: B27
  article-title: Förster resonance energy transfer (FRET) and applications thereof
  publication-title: Anal. Methods
  doi: 10.1039/d0ay01961e
  contributor:
    fullname: Kaur
– volume: 133
  start-page: 2431
  year: 2020
  ident: B70
  article-title: Development and validation of high-throughput and low-cost STARP assays for genes underpinning economically important traits in wheat
  publication-title: Theor. Appl. Genet.
  doi: 10.1007/s00122-020-03609-w
  contributor:
    fullname: Wu
– volume: 47
  start-page: 486
  year: 2001
  ident: B33
  article-title: Simultaneous absolute quantification of target and control templates by real-time fluorescence reverse transcription-PCR using 4-(4′-dimethylaminophenylazo)benzoic acid as a dark quencher dye
  publication-title: Clin. Chem.
  doi: 10.1093/clinchem/47.3.486
  contributor:
    fullname: Kreuzer
– volume: 19
  start-page: 55
  year: 2019
  ident: B17
  article-title: Lack of specificity associated with using molecular beacons in loop mediated amplification assays
  publication-title: BMC Biotechnol.
  doi: 10.1186/s12896-019-0549-z
  contributor:
    fullname: Hardinge
– volume: 17
  start-page: 254
  year: 2017
  ident: B19
  article-title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping
  publication-title: BMC Plant Biol.
  doi: 10.1186/s12870-017-1197-x
  contributor:
    fullname: Jatayev
– ident: B21
– start-page: 825
  volume-title: The Recent Topics in Genetic Polymorphisms
  year: 2020
  ident: B44
  article-title: Single nucleotide polymorphisms (SNPs) in plant genetics and breeding,
  doi: 10.5772/intechopen.91886
  contributor:
    fullname: Morgil
– volume: 9
  start-page: 289
  year: 2007
  ident: B32
  article-title: SNP genotyping: technologies and biomedical applications
  publication-title: Annu. Rev. Biomed. Eng.
  doi: 10.1146/annurev.bioeng.9.060906.152037
  contributor:
    fullname: Kim
– volume: 95
  start-page: 1460
  year: 1998
  ident: B56
  article-title: A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbour thermodynamics
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.95.4.1460
  contributor:
    fullname: SantaLucia
– volume: 138
  start-page: 309
  year: 2019
  ident: B37
  article-title: Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp
  publication-title: chinensis Makino. Plant Breed.
  doi: 10.1111/pbr.12686
  contributor:
    fullname: Li
– volume: 14
  start-page: 28
  year: 2018
  ident: B4
  article-title: Comparison of three PCR-based assays for SNP genotyping in plants
  publication-title: Plant Methods
  doi: 10.1186/s13007-018-0295-6
  contributor:
    fullname: Broccanello
– volume: 31
  start-page: 1106
  year: 2001
  ident: B9
  article-title: DNA probes using fluorescence resonance energy transfer (FRET): Designs and applications
  publication-title: BioTechniques
  doi: 10.2144/01315rv02
  contributor:
    fullname: Didenko
– volume: 1
  start-page: 219
  year: 2000
  ident: B12
  article-title: Large-scale SNP scoring from unamplified genomic DNA
  publication-title: Pharmacogenomics
  doi: 10.1517/14622416.1.2.219
  contributor:
    fullname: Fors
– volume: 9
  start-page: 104
  year: 2018
  ident: B71
  article-title: Development and applications of a high throughput genotyping tool for polyploid crops: Single nucleotide polymorphism (SNP) array
  publication-title: Front. Plant Sci.
  doi: 10.3389/fpls.2018.00104
  contributor:
    fullname: You
– volume: 12
  start-page: 109
  year: 2010
  ident: B62
  article-title: The effect of primer-template mismatches on the detection and quantification of nucleic acids using the 5′ nuclease assay
  publication-title: J. Mol. Diagn.
  doi: 10.2353/jmoldx.2010.090035
  contributor:
    fullname: Stadhouders
– volume: 9
  start-page: e10581
  year: 2021
  ident: B43
  article-title: Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study
  publication-title: PeerJ
  doi: 10.7717/peerj.10581
  contributor:
    fullname: Massa
– volume: 3
  start-page: 77
  year: 2003
  ident: B7
  article-title: Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput
  publication-title: Pharmacogenomics J.
  doi: 10.1038/sj.tpj.6500167
  contributor:
    fullname: Chen
– start-page: 327
  volume-title: Advances in Breeding Techniques for Cereal Crops
  year: 2019
  ident: B58
  article-title: Development of Single nucleotide polymorphism (SNP) markers for cereal breeding and crop research: current methods and future prospects,
  doi: 10.19103/AS.2019.0051.16
  contributor:
    fullname: Schramm
– start-page: 223
  volume-title: PCR Primer Design. Methods in Molecular Biology
  year: 2022
  ident: B24
  article-title: A guide to using FASTPCR software for PCR, in silico PCR, and oligonucleotide analysis
  doi: 10.1007/978-1-0716-1799-1_16
  contributor:
    fullname: Kalendar
– volume: 4
  start-page: 183
  year: 2009
  ident: B11
  article-title: Laboratory methods for high-throughput genotyping
  publication-title: Cold Spring Harb. Protoc.
  doi: 10.1101/pdb.top62
  contributor:
    fullname: Edenberg
– volume: 396
  start-page: 2023
  year: 2010
  ident: B14
  article-title: Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection
  publication-title: Anal. Bioanal. Chem.
  doi: 10.1007/s00216-009-3418-0
  contributor:
    fullname: Gašparič
– volume: 77
  start-page: 267
  year: 2009
  ident: B69
  article-title: Quantitative effects of position and type of single mismatch on single base primer extension
  publication-title: J. Microbiol. Methods
  doi: 10.1016/j.mimet.2009.03.001
  contributor:
    fullname: Wu
– volume: 2
  start-page: 14065
  year: 2015
  ident: B67
  article-title: Developing single nucleotide polymorphism (SNP) markers from transcriptome sequences for identification of longan (Dimocarpus longan) germplasm
  publication-title: Hort. Res.
  doi: 10.1038/hortres.2014.65
  contributor:
    fullname: Wang
– volume: 28
  start-page: 3752
  year: 2000
  ident: B64
  article-title: Mode of action and application of Scorpion primers to mutataon detection
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/28.19.3752
  contributor:
    fullname: Thelwell
– volume: 129
  start-page: 1843
  year: 2016
  ident: B51
  article-title: Development and validation of KASP assays for genes underpinning key economic traits in bread wheat
  publication-title: Theor. Appl. Genet.
  doi: 10.1007/s00122-016-2743-x
  contributor:
    fullname: Rasheed
– start-page: 45
  volume-title: Plant Breeding from Laboratories to Fields
  year: 2013
  ident: B22
  article-title: Molecular markers and marker-assisted breeding in plants,
  doi: 10.5772/52583
  contributor:
    fullname: Jiang
– volume: 11
  start-page: 80
  year: 2011
  ident: B10
  article-title: RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers
  publication-title: BMC Biotechnol.
  doi: 10.1186/1472-6750-11-80
  contributor:
    fullname: Dobosy
– volume: 17
  start-page: 95
  year: 2014
  ident: B53
  article-title: One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.)
  publication-title: Electron. J. Biotechnol.
  doi: 10.1016/j.ejbt.2014.02.003
  contributor:
    fullname: Rosas
– volume: 10
  start-page: 277
  year: 2010
  ident: B59
  article-title: HvNax3—a locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp
  publication-title: spontaneum). Funct. Integr. Genomics
  doi: 10.1007/s10142-009-0153-8
  contributor:
    fullname: Shavrukov
– volume: 109
  start-page: 312
  year: 2017
  ident: B26
  article-title: FastPCR: an in silico tool for fast primer and probe design and advanced sequence analysis
  publication-title: Genomics
  doi: 10.1016/j.ygeno.2017.05.005
  contributor:
    fullname: Kalendar
– volume: 25
  start-page: 2516
  year: 1997
  ident: B46
  article-title: A closed tube format for amplification and detection of DNA based on energy transfer
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/25.12.2516
  contributor:
    fullname: Nazarenko
– volume: 8
  start-page: 34
  year: 2012
  ident: B38
  article-title: An improved allele-specific PCR primer design method for SNP marker analysis and its application
  publication-title: Plant Methods
  doi: 10.1186/1746-4811-8-34
  contributor:
    fullname: Liu
– volume: 7
  start-page: 365
  year: 2006
  ident: B57
  article-title: Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real-time PCR.
  publication-title: Mol. Plant Pathol.
  doi: 10.1111/j.1364-3703.2006.00345.x
  contributor:
    fullname: Schena
– volume: 5
  start-page: 1
  year: 2009
  ident: B6
  article-title: Simple allele-discriminating PCR for cost-effective and rapid genotyping and mapping
  publication-title: Plant Methods
  doi: 10.1186/1746-4811-5-1
  contributor:
    fullname: Bui
– volume: 39
  start-page: 913
  year: 2017
  ident: B8
  article-title: Molecular authentication of two medicinal plants Ligularia fischeri and Ligularia stenocephala using allele-specific PCR (AS-PCR) strategy
  publication-title: Genes Genomics
  doi: 10.1007/s13258-017-0554-3
  contributor:
    fullname: Choi
– volume: 158
  start-page: 112983
  year: 2020
  ident: B23
  article-title: Marker-assisted selection for fast-track breeding of high oleic lines in safflower (Carthamus tinctorious L.).
  publication-title: Ind. Crops Prod.
  doi: 10.1016/j.indcrop.2020.112983
  contributor:
    fullname: Kadirvel
– volume: 10
  start-page: 63
  year: 2019
  ident: B75
  article-title: The general transcription repressor TaDr1 is co-expressed with TaVrn1 and TaFT1 in bread wheat under drought
  publication-title: Front. Genet.
  doi: 10.3389/fgene.2019.00063
  contributor:
    fullname: Zotova
– start-page: 123
  volume-title: Next Generation Sequencing and Whole Genome Selection in Aquaculture
  year: 2011
  ident: B47
  article-title: SNP genotyping platforms,
  doi: 10.1002/9780470958964.ch8
  contributor:
    fullname: Peatman
– volume: 3
  start-page: 254
  year: 2013
  ident: B74
  article-title: The use of allele-specific markers of the Ppd and Vrn genes for predicting growing-season duration in barley cultivars
  publication-title: Russ. J. Genet.: Appl. Res.
  doi: 10.1134/S2079059713040114
  contributor:
    fullname: Zlotina
– volume: 20
  start-page: 160
  year: 2019
  ident: B72
  article-title: A high-throughput SNP discovery strategy for RNA-seq data
  publication-title: BMC Genomics
  doi: 10.1186/s12864-019-5533-4
  contributor:
    fullname: Zhao
– volume: 77
  start-page: 2477
  year: 2021
  ident: B5
  article-title: A needle in a seedstack: an improved method for detection of rare alleles in bulk seed testing through KASP
  publication-title: Pest Manag. Sci.
  doi: 10.1002/ps.6278
  contributor:
    fullname: Brusa
– start-page: 75
  volume-title: Crop Breeding. Methods in Molecular Biology
  year: 2021
  ident: B73
  article-title: Specific-locus amplified fragment sequencing (SLAF-Seq) as high-throughput SNP genotyping methods,
  doi: 10.1007/978-1-0716-1201-9_6
  contributor:
    fullname: Zhu
– volume: 2
  start-page: 195
  year: 2014
  ident: B65
  article-title: High-throughput SNP genotyping to accelerate crop improvement
  publication-title: Plant Breed. Biotech.
  doi: 10.9787/PBB.2014.2.3.195
  contributor:
    fullname: Thomson
– volume: 10
  start-page: 1047
  year: 2017
  ident: B50
  article-title: Crop breeding chips and genotyping platforms: progress, challenges, and perspectives
  publication-title: Mol. Plant.
  doi: 10.1016/j.molp.2017.06.008
  contributor:
    fullname: Rasheed
– volume: 10
  start-page: 117
  year: 2009
  ident: B49
  article-title: Genotyping technologies for genetic research
  publication-title: Annu. Rev. Genomics Hum. Genet.
  doi: 10.1146/annurev-genom-082908-150116
  contributor:
    fullname: Ragoussis
– volume: 48
  start-page: 409
  ident: B28
  article-title: Utilization of KASP technology for wheat improvement
  publication-title: Cereal Res. Commun.
  doi: 10.1007/s42976-020-00057-6
  contributor:
    fullname: Kaur
– start-page: 219
  volume-title: High Throughput Screening for Food Safety Assessment
  year: 2015
  ident: B39
  article-title: Fluorescence-based real-time quantitative polymerase chain reaction (qPCR) technologies for high throughput screening of pathogens
  doi: 10.1016/B978-0-85709-801-6.00009-5
  contributor:
    fullname: Löfström
– volume-title: Plant Breeding—Current and Future Views
  year: 2020
  ident: B66
  article-title: Single nucleotide polymorphisms: a modern tool to screen plants for desirable traits,
  contributor:
    fullname: Udoh
– volume: 20
  start-page: 156
  year: 2020
  ident: B3
  article-title: Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan
  publication-title: BMC Plant Biol
  doi: 10.1186/s12870-020-02332-4
  contributor:
    fullname: Baidyussen
– volume: 3
  start-page: e2882
  year: 2008
  ident: B13
  article-title: High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0002882
  contributor:
    fullname: Fuhrman
– volume: 21
  start-page: 8296
  year: 2020
  ident: B77
  article-title: TaDrAp1 and TaDrAp2, partner genes of a transcription repressor, coordinate plant development and drought tolerance in spelt and bread wheat
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms21218296
  contributor:
    fullname: Zotova
– volume: 40
  start-page: 9
  year: 2020
  ident: B41
  article-title: A direct PCR-based SNP marker-assisted selection system (D-MAS) for different crops
  publication-title: Mol. Breed.
  doi: 10.1007/s11032-019-1091-3
  contributor:
    fullname: Lu
– start-page: 57
  volume-title: Molecular Plant Taxonomy. Methods in Molecular Biology
  year: 2021
  ident: B25
  article-title: Isolation and purification of DNA from complicated biological samples,
  doi: 10.1007/978-1-0716-0997-2_3
  contributor:
    fullname: Kalendar
– volume: 11
  start-page: 4748
  year: 2021
  ident: B68
  article-title: Development and validation of a real-time PCR assay to detect Cannabis sativa in food
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-021-83908-4
  contributor:
    fullname: Weck
– volume: 112
  start-page: 1115
  year: 2006
  ident: B15
  article-title: Utilization of the three high-throughput SNP genotyping methods, the GOOD assay, Amplifluor and TaqMan, in diploid and polyploid plants
  publication-title: Theor. Appl. Genet.
  doi: 10.1007/s00122-006-0213-6
  contributor:
    fullname: Giancola
– volume: 35
  start-page: 57
  year: 2018
  ident: B55
  article-title: Genotyping-by-sequencing based single nucleotide polymorphisms enabled Kompetitive Allele Specific PCR marker development in mutant Rubus genotypes
  publication-title: Electron. J. Biotechnol.
  doi: 10.1016/j.ejbt.2018.08.001
  contributor:
    fullname: Ryu
– volume: 55
  start-page: 4312
  year: 2007
  ident: B35
  article-title: Comparison of real-time PCR detection chemistries and cycling modes using Mon810 event-specific assays as model
  publication-title: J. Agric. Food Chem.
  doi: 10.1021/jf063725g
  contributor:
    fullname: La Paz
– volume: 244
  start-page: 225
  year: 2019
  ident: B54
  article-title: Single nucleotide polymorphism (SNP) discovery through genotyping-by-sequencing (GBS) and genetic characterization of Dendrobium mutants and cultivars
  publication-title: Sci. Hortic.
  doi: 10.1016/j.scienta.2018.09.053
  contributor:
    fullname: Ryu
– volume: 14
  start-page: e0217222
  year: 2019
  ident: B2
  article-title: Comparison of TaqMan, KASP and rhAmp SNP genotyping platforms in hexaploid wheat
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0217222
  contributor:
    fullname: Ayalew
– ident: B42
– volume: 130
  start-page: 597
  year: 2017
  ident: B40
  article-title: An innovative SNP genotyping method adapting to multiple platforms and throughputs
  publication-title: Theor. Appl. Genet
  doi: 10.1007/s00122-016-2838-4
  contributor:
    fullname: Long
– volume: 7
  start-page: 1736
  year: 2016
  ident: B60
  article-title: Expression level of the DREB2-type gene, identified with Amplifluor SNP markers, correlates with performance, and tolerance to dehydration in bread wheat cultivars from Northern Kazakhstan
  publication-title: Front. Plant Sci.
  doi: 10.3389/fpls.2016.01736
  contributor:
    fullname: Shavrukov
– volume: 27
  start-page: 425
  year: 2016
  ident: B36
  article-title: Allele-specific quantitative PCR for accurate, rapid, and cost-effective genotyping
  publication-title: Hum. Gene Ther.
  doi: 10.1089/hum.2016.011
  contributor:
    fullname: Lee
– volume: 10
  start-page: 40
  year: 2019
  ident: B30
  article-title: Intracellular vesicle trafficking genes, RabC-GTP, are highly expressed under salinity and rapid dehydration but down-regulated by drought in leaves of chickpea (Cicer arietinum L.).
  publication-title: Front. Genet.
  doi: 10.3389/fgene.2019.00040
  contributor:
    fullname: Khassanova
– start-page: 215
  volume-title: Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols. Methods in Molecular Biology
  year: 2006
  ident: B31
  article-title: High-throughput genotyping with energy transfer-labeled primers,
  doi: 10.1385/1-59745-069-3:215
  contributor:
    fullname: Khripin
– volume: 2012
  start-page: 831460
  year: 2012
  ident: B34
  article-title: SNP discovery through next-generation sequencing and its applications
  publication-title: Int. J. Plant Genomics
  doi: 10.1155/2012/831460
  contributor:
    fullname: Kumar
– volume: 20
  start-page: 183
  year: 2020
  ident: B63
  article-title: Salt-induced expression of intracellular vesicle tracking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.).
  publication-title: BMC Plant Biol.
  doi: 10.1186/s12870-020-02331-5
  contributor:
    fullname: Sweetman
SSID ssj0000500997
Score 2.4060583
Snippet The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism...
The proposed method is a modified and improved version of the existing “Allele-specific q-PCR” (ASQ) method for genotyping of single nucleotide polymorphism...
SourceID doaj
pubmedcentral
proquest
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 747886
SubjectTerms allele-specific primers
fluorescence and quenching
FRET-based method
genotyping
Plant Science
qPCR and plate reader instruments
single nucleotide polymorphism (SNP)
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA4iHryIb-uLCF48VNOmzePoiqsoivgAbyVJE1xY2lXWgzd_iP45f4kz7Sq7Injx2jYkfJNmviEz3xCyG4TJ0zILcZlgSY7naWxtsLHhCvytSTwMxGyLS3F6l53d5_djrb4wJ6yVB26BO-Dc5T63nmtUavPSWM2DLIWTyBSUa05flo4FU62qN1If2d5LQhSmD8Kgj-rcabLfKMaLCT_UyPX_xjF_pkqO-Z7uPJkbkUZ62C52gUz5apHMdGogdi9L5PyiLnsBqCT9eH077PfBkcRNW_nQc_Tx6uj64_WdXjSdoilQVHpzeUVPfFUPX7BWinbAj5W0rmj3-vh2mdx1j2-PTuNRj4TYZSIdxsFxCZyLBWOSkAUvAgZ4QjgXjM6CzSRzWvHgmcYrVC-U1c4DB8m10FYlfIVMV3Xl1whVzCsDf7hMBdAM7QBwoB-CWZ6B4XIfkb0vxIpBK4VRQAiB6BaIboHoFi26EekgpN_foYh18wBMW4xMW_xl2ojsfBmkgE2PNxmm8vUzzASkjcNhk7GIrLYG-p6K56jKx3hE5ITpJtYy-abqPTTC2koqrLxd_4_Fb5DZFCslGGYMbpLp4dOz3wL-MrTbzVb9BMr47Y4
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Nb9QwEB1B4cAF8U0oIFNx4ZDixI5jHxDqVt1WSFtVpSvtLbIdG1ZaJe2ylbr_npkkLSzaA9ckjpOZOO-NPPMG4GNUtshrGdM6o5KcIPLUuehSKzTirc0CDqRsi1N1MpXfZsXsT3n0YMBfW0M76ic1XS72b67WX3HBf6GIE_H2c7xckPB2nu13YvDqPjzIERcpwWsykP1e6ZvoUNdtRSmZSpXP-n3LrTfZwKlOzn8bB_03lfIvbBo_gccDqWQH_VfwFO6F5hk8HLVI_NbP4XjS1vOIVJPtHSwWCDNp13Q-zj27Ojs832OTros0Q_rKvp-esePQtKs11VGxEWJczdqGjc-PLl7AdHx0cXiSDv0TUo8vt0qjFyXyMR6tzaKMQUUK_pTyPlojo5Ml90aLGLih7dWgtDM-ID8pjDJOZ-Il7DRtE14D0zxoi6u_zBVSEONNgaDGFXdColOLkMCnW2tVl71MRoXhBVm2IstWZNmqt2wCIzLn3XUkcN0daJc_qmG94EhfhMIFYUigL5TWGRHLWvmSCKL2CXy4dUaFC4J2OWwT2mucCQmdwB-R5Am86p1zN5UoSLGPiwTKDbdtPMvmmWb-sxPd1qWmqtw3_zHvLjzKqUiCU7LgW9hZLa_DO6QuK_e--yJ_A3Nf6RM
  priority: 102
  providerName: Scholars Portal
Title Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET
URI https://www.ncbi.nlm.nih.gov/pubmed/35082803
https://search.proquest.com/docview/2623325740
https://pubmed.ncbi.nlm.nih.gov/PMC8784781
https://doaj.org/article/33c5e5be395443e7ab93f7d6c711298c
Volume 12
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NTtwwELYA9dBLVfqb0iJX6qWH7Cax458ju2JBoEUrCtLeotix25WWZIuWAzcehL4cT8KMkyC26qkXHxJbtmYmmW_kmW8I-eZFmWcV93GVYkmOY1lsjDdxyRT42zJ1sBCzLc7E8SU_mefzLZL3tTAhad-axaBeXg3qxa-QW7m6ssM-T2w4m46VVFghOdwm2-B-n4XoLaE3oh7ZXklCAKaHfrVEYu4sHQSyeOxaxHKkbus7ZXXeKJD2_wtp_p0w-cwDTV6TVx10pAftEXfJlqvfkBejBuDd7VtyOm2qhQdASR_u7g-WS3AncWgu7xeW_p6Nzx_u_tBp6BdNAajSH2czeuTqZn2LFVN0BN6sok1NJ-eHF-_I5eTwYnwcd50SYstFto69ZRKQV-LLMvXcO-ExzBPCWl9q7g2XidWKeZdovEh1QhltHSCRXAttVMrek526qd1HQlXiVAnfucwEgA1tdQ7uKxGJYRzUl7uIfO8lVqxaQowCAgkUdIGCLlDQRSvoiIxQpE_zkMo6PGiufxadQmGlzV1uHNNIxedkaTTzshJWIhRUNiJfe4UUYPp4n1HWrrmBnQC6Mfjl8CQiH1oFPW3VKzgickN1G2fZfAPWFui1O-v69N8r98jLDIskEkwW_Ex21tc37gtAl7XZDyE_jEfzFMYpV_vBeB8Bx3XxDQ
link.rule.ids 230,314,727,780,784,864,885,2102,24318,27924,27925,53791,53793
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NbtswDCa6bsB6GfY_71cDdtnBiW1ZknVsgmbZ2gRBlwK9GZYsbQFSOy3SQ299kO3l-iQj7bhohp12tU1IICnzI0R-BPjkZSGSMvVhGVNLjuNJaIw3YcEzjLdF7FCQqi2mcnySfjsVpzsgul6YpmjfmkWvWp71qsXPprZydWb7XZ1YfzYZZiqjDsn-PbgvuNLxnSS9pfQm3KPaS0lMwXTfr5ZEzZ3EvYYunuYWcUHkbd2srE08amj7_4U1_y6ZvBODRo_h0QY8sv12k09gx1VP4cGgRoB39QwOJ3W58Agp2c31r_3lEgNK2IyX9wvLzmfD45vr32zSTIxmCFXZ9-mMfXFVvb6inik2wHhWsrpio-OD-XM4GR3Mh-NwMyshtKlM1qG3XCH2inxRxD71TnpK9KS01hc69SZVkdUZ9y7SdJXqZGa0dYhFhJbaZDF_AbtVXblXwLLIZQWedJVIhBvaaoEBLJKR4SkaULgAPncay1ctJUaOqQQpOidF56TovFV0AANS6e13RGbdPKgvfuQbk6KkFU4YxzWR8TlVGM29KqVVBAYzG8DHziA5Oj_daBSVqy9xJQRvHH86aRTAy9ZAt0t1Bg5AbZluay_bb9DfGoLtjX-9_m_JD_BwPJ8c5Udfp4dvYC-hlomISgffwu764tK9QyCzNu8bt_0Docfxog
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3NbtQwELagIMQF8VsCBYzEhUM2P04c-9hduhTKrlallXqLbMduV9omS7U99NYHgZfrk3TGSapdxIlrkpGtmXHmG3nmG0I-Oa7ytMpcWCXYkmNZGmrtdKiYgHirEguCWG0x5fvH2feT_GRt1Jcv2jd6PqgX54N6fuZrK5fnJurrxKLZZCQKgR2S0bJy0X3yIGfgZGuJekvrjdinaC8mIQ2TkVsukJ47TQaeMh5nF7EcCdz6eVldTPLU_f_Cm3-XTa7FofFT8qQDkHS33egzcs_Wz8nDYQMg7-oFOZg01dwBrKQ31793FwsIKqEfMe_mhv6ajQ5vrv_QiZ8aTQGu0p_TGf1q62Z1hX1TdAgxraJNTceHe0cvyfF472i0H3bzEkKT8XQVOsMKwF-xUypxmbPcYbLHuTFOyczprIiNFMzZWOJ1quVCS2MBj-SSSy0S9ops1U1tXxMqYisUnPYi5QA5pJE5BLGYx5plYMTcBuRzr7Fy2dJilJBOoKJLVHSJii5bRQdkiCq9-w4Jrf2D5uK07MwKkia3ubZMIiGfLZSWzBUVNwUCQmEC8rE3SAkHAG81VG2bS1gJAByDH08WB2S7NdDdUr2BA1JsmG5jL5tvwOc8yXbnY2_-W_IDeTT7Mi5_fJsevCWPU-yaiLF6cIdsrS4u7TvAMiv93nvtLSQ28rU
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Modified+%22Allele-Specific+qPCR%22+Method+for+SNP+Genotyping+Based+on+FRET&rft.jtitle=Frontiers+in+plant+science&rft.au=Kalendar%2C+Ruslan&rft.au=Baidyussen%2C+Akmaral&rft.au=Serikbay%2C+Dauren&rft.au=Zotova%2C+Lyudmila&rft.date=2022-01-10&rft.issn=1664-462X&rft.eissn=1664-462X&rft.volume=12&rft.spage=747886&rft.epage=747886&rft_id=info:doi/10.3389%2Ffpls.2021.747886&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-462X&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-462X&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-462X&client=summon