Analysis of Fecal Lactobacillus Community Structure in Patients with Early Rheumatoid Arthritis

• Published in: Current microbiology Vol. 67; no. 2; pp. 170 - 176
• Main Authors: Liu, Xiaofei, Zou, Qinghua, Zeng, Benhua, Fang, Yongfei, Wei, Hong
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.08.2013; Springer Nature B.V
• Subjects: Abundance; Adult; Arthritis, Rheumatoid; Arthritis, Rheumatoid - microbiology; Bacteria; Bacteriology; Biodiversity; Biomedical and Life Sciences; Biotechnology; classification; community development; Community structure; denaturing gradient gel electrophoresis; DNA; Feces; Feces - microbiology; Female; Gastrointestinal Tract; Gastrointestinal Tract - microbiology; genetics; Humans; intestinal microorganisms; isolation & purification; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Life Sciences; Male; Microbiology; Middle Aged; Molecular Sequence Data; patients; Phylogeny; Polymerase Chain Reaction; quantitative analysis; quantitative polymerase chain reaction; Rheumatoid arthritis; ribosomal RNA; Species diversity; Rheumatoid Arthritis; Intestinal Microflora; Rheumatoid Arthritis Patient; Early Rheumatoid Arthritis; Lactobacillus

The objective of this study was to analyze human fecal Lactobacillus community and its relationship with rheumatoid arthritis. Samples taken from rheumatoid arthritis (RA) patients and healthy individuals were analyzed by quantitative real-time PCR. Bacterial DNA was extracted from feces, and amplic...