Identification of Specific Joint-Inflammatogenic Cell-Free DNA Molecules From Synovial Fluids of Patients With Rheumatoid Arthritis

• Published in: Frontiers in immunology Vol. 11; p. 662
• Main Authors: Dong, Cong, Liu, Yu, Sun, Chengxin, Liang, Huiyi, Dai, Lie, Shen, Jun, Wei, Song, Guo, Shixin, Leong, Kam W., Chen, Yongming, Wei, Lai, Liu, Lixin
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 28.04.2020
• Subjects: Adult; Animals; Arthritis, Rheumatoid - blood; autoimmunity; Biomarkers - blood; Case-Control Studies; cell-free DNA; Cell-Free Nucleic Acids - blood; Cell-Free Nucleic Acids - genetics; Cell-Free Nucleic Acids - isolation & purification; Cell-Free Nucleic Acids - pharmacology; Cohort Studies; CpG Islands; CpG-motif; Disease Models, Animal; Female; Humans; Immunology; inflammation; Inflammation - chemically induced; Inflammation - immunology; Male; Mice; Mice, Inbred BALB C; Middle Aged; Monocytes - drug effects; Monocytes - immunology; Osteoarthritis - blood; rheumatoid arthritis; Synovial Fluid - chemistry; Synovial Fluid - immunology; Synoviocytes - drug effects; Synoviocytes - immunology; THP-1 Cells; cell-free DNA; CpG-motif; autoimmunity; inflammation; rheumatoid arthritis

Elevated cell-free DNA (cfDNA) levels in the plasma and synovial fluid of rheumatoid arthritis (RA) patients are proposed to be pathologically relevant. However, direct evidence to support this perception is lacking, and molecular feature of the cfDNA molecules with assumed pathological function is...