Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol

• Published in: Analytical biochemistry Vol. 404; no. 2; pp. 155 - 164
• Main Authors: Dietmair, Stefanie, Timmins, Nicholas E., Gray, Peter P., Nielsen, Lars K., Krömer, Jens O.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2010
• Subjects: Acetonitriles - chemistry; Animals; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Cricetulus; Extraction; Mammalian cell culture; Metabolome; Metabolomics; Metabolomics - methods; Methanol - chemistry; Quenching; Sodium Chloride - chemistry; Metabolomics; Extraction; Mammalian cell culture; Quenching

Metabolomics aims to quantify all metabolites within an organism, thereby providing valuable insight into the metabolism of cells. To study intracellular metabolites, they are first extracted from the cells. The ideal extraction procedure should immediately quench metabolism and quantitatively extra...