Application of Intravoxel Incoherent Motion Diffusion-Weighted Imaging in Predicting and Monitoring Early Efficacy of Anti-Angiogenic Therapy in the C6 Glioma Rat Model

• Published in: Frontiers in oncology Vol. 11; p. 842169
• Main Authors: Hou, Weishu, Xue, Yangyang, Qian, Yinfeng, Pan, Hongli, Xu, Man, Shen, Yujun, Li, Xiaohu, Yu, Yongqiang
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 28.01.2022
• Subjects: angiogenesis inhibitors; glioma; intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI); Oncology; therapy; vascular normalization; intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI); vascular normalization; glioma; angiogenesis inhibitors; therapy
• ISSN: 2234-943X; 2234-943X
• DOI: 10.3389/fonc.2021.842169

To investigate the feasibility of intravoxel incoherent motion (IVIM) diffusion-weighted imaging (DWI) in evaluating early effects of anti-angiogenic therapy in the C6 glioma rat model. Twenty-six rats of the C6 glioma model were randomly divided into a treatment group (received bevacizumab) and a control group (physiological saline). IVIM-DWI was performed on days 0, 1, 3, 5, and 7 after anti-angiogenic therapy and tumor growth and IVIM-DWI parameters were dynamically observed. Hematoxylin and eosin, CD34 microvessel density (MVD), proliferation of cell nuclear antigen (PCNA), and Hif-α staining were performed on day 7. One-way ANOVA was used to compare intra-group differences and an independent-samples -test was used to compare inter-group differences of MRI parameters. Correlations between IVIM-DWI parameters, tumor size, and pathological results were analyzed. The relative change in tumor volume (ΔVolume) in the two groups differed significantly on days 5 and 7 ( = 0.038 and < 0.001). The perfusion-related parameters D*- and f-values decreased in the treatment group and demonstrated significant differences compared with the control group on days 3, 5, and 7 ( = 0.033, < 0.001, and < 0.001, respectively). The diffusion-related parameters ADC and D-values increased in the treatment group and were found to be significantly differently different from the control group on days 5 and 7 (both < 0.001). The initial D-value showed a negative correlation with ΔVolume (γ = -0.744, < 0.001), whereas the initial D*-value and relative change of D-value had a positive correlation with ΔVolume (γ = 0.718, < 0.001 and γ = 0.800, < 0.001, respectively). MVD was strongly positively correlated with D*-value ( = 0.886, = 0.019), PCNA was negatively correlated with ADC- and D-values ( = -0.848, = 0.033; and = -0.928 = 0.008, respectively), and Hif-1α was strongly negatively correlated with D*-value ( = -0.879, = 0.010). IVIM-DWI was sensitive and accurate in predicting and monitoring the effects of early anti-angiogenesis therapy in a C6 glioma rat model.