In vitro production of insulin-responsive skeletal muscle tissue from mouse embryonic stem cells by spermine-induced differentiation method
The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of...
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| Published in | Human cell : official journal of Human Cell Research Society Vol. 30; no. 3; pp. 162 - 168 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Tokyo
Springer Japan
01.07.2017
Springer Nature B.V |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1749-0774 0914-7470 1749-0774 |
| DOI | 10.1007/s13577-017-0176-8 |
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| Abstract | The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[
N
-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-
d
-glucose (2-NBDG) and its
l
-glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses. |
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| AbstractList | The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) and its L-glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses. The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) and its L-glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses.The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) and its L-glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses. The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[ N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy- d -glucose (2-NBDG) and its l -glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses. The treatment of an embryoid body with spermine for a short duration can trigger the generation of a 3-dimensional multilayer myotube sheet (MMTS) that shows pulsatile activity. MMTS was previously characterized as a model of skeletal muscle tissue. In the present work, the insulin responsiveness of MMTS was investigated because it is an essential function for a model of skeletal muscle. The glucose uptake activity of MMTS was analyzed by confocal microscopy using fluorescent glucose analogs, namely 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy- d -glucose (2-NBDG) and its l -glucose counterpart, 2-NBDLG. The specific uptake rate of glucose was estimated from the difference between the fluorescent signals of 2-NBDG and 2-NBDLG. It was enhanced by insulin stimulation to 3.6 times higher than the control without insulin, and this insulin responsiveness was maintained for 5 days. The advantages of the 3-dimensional structure of MMTS are discussed in the contexts of its potential in vivo and in vitro uses. |
| Author | Saito, Mikako Nakagawa, Shota Ishida, Ayano |
| Author_xml | – sequence: 1 givenname: Mikako surname: Saito fullname: Saito, Mikako email: mikako@cc.tuat.ac.jp organization: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology – sequence: 2 givenname: Ayano surname: Ishida fullname: Ishida, Ayano organization: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology – sequence: 3 givenname: Shota surname: Nakagawa fullname: Nakagawa, Shota organization: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28577277$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1038/srep22222 10.1073/pnas.0405315101 10.1016/bs.pmbts.2016.12.011 10.1007/s00125-016-4071-8 10.1172/JCI7917 10.1038/nprot.2007.76 10.1007/978-1-4939-6771-1_11 10.1016/j.foodchem.2013.04.042 10.1016/0304-4165(95)00153-0 10.1139/y02-043 10.2337/db09-0796 10.7717/peerj.2231 10.1016/j.clnu.2015.05.005 10.2337/db16-0997 10.2217/rme-2015-0045 10.3390/ijms151018677 10.1007/s11626-014-9799-1 10.1111/j.1432-0436.2008.00294.x 10.1096/fj.09-137174 10.1007/s11307-005-0011-6 10.1007/s12015-011-9258-2 10.1002/cbf.3081 10.5796/electrochemistry.80.299 10.1371/journal.pone.0154415 10.1007/978-1-4939-6771-1_12 10.1016/j.jdiacomp.2016.06.014 10.1186/s12860-017-0131-2 |
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| Keywords | Insulin-stimulated glucose uptake Skeletal muscle tissue Multilayer myotube sheet Spermine Fluorescent glucose analog |
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| References | Yoshioka, Takahashi, Homma (CR9) 1996; 1289 Santos, Benite-Ribeiro, Queiroz, Duarte (CR27) 2014; 32 Bertuzzi, Conte, Mingrone, Papa, Salinari, Sinisgalli (CR5) 2016; 11 Ball, Bailey, Stewart, Vogels, Westcott (CR10) 2002; 80 Hashimoto, Tamaki, Hirata, Uchiyama, Sato, Mochida (CR16) 2016; 4 Funabashi, Ogino, Saito, Matsuoka (CR15) 2012; 80 Haywood, Cordell, Tang (CR24) 2017; 66 Al-Bayati, Lukka, Brown, Walker (CR4) 2016; 30 Culberson (CR17) 2017; 146 Hall, Hall, Cadwallader (CR19) 2017; 1556 Mizuno, Chang, Umeda (CR21) 2010; 24 Tierney, Sacco (CR22) 2017; 1556 Bañuls, Rovira-Llopis, Falcón (CR26) 2016; 35 Satoh (CR28) 2014; 15 Sasaki, Matsuoka, Saito (CR7) 2008; 76 Kido, Burks, Withers (CR1) 2000; 105 Yamada, Saito, Matsuoka, Inagaki (CR13) 2007; 2 Hernández-Mijares, Bañuls, Peris (CR25) 2013; 141 Assi, Foster, He (CR18) 2016; 11 González-Rodríguez, Mas-Gutierrez, Sanz-González (CR2) 2010; 59 Yamamoto, Nishiuchi, Teshima, Matsuoka, Yamada (CR14) 2008; 49 Bernardinelli, Magistretti, Chatton (CR11) 2004; 101 CR23 Darabi, Pan, Bosnakovski, Baik, Kyba, Perlingeiro (CR20) 2011; 7 O’Neil, Wu, Mullani (CR12) 2005; 7 Obanda, Ribnicky, Yu, Stephens, Cefalu (CR6) 2016; 6 Saito, Abe, Ishida, Nakagawa, Matsuoka (CR8) 2014; 50 Sato, Kubota, Kubota (CR3) 2016; 59 H Hashimoto (176_CR16) 2016; 4 T Yamamoto (176_CR14) 2008; 49 JW Culberson (176_CR17) 2017; 146 NJ Haywood (176_CR24) 2017; 66 R Assi (176_CR18) 2016; 11 M Saito (176_CR8) 2014; 50 R Darabi (176_CR20) 2011; 7 M Tierney (176_CR22) 2017; 1556 A Al-Bayati (176_CR4) 2016; 30 RG O’Neil (176_CR12) 2005; 7 H Sato (176_CR3) 2016; 59 Y Yamada (176_CR13) 2007; 2 Á González-Rodríguez (176_CR2) 2010; 59 T Satoh (176_CR28) 2014; 15 A Hernández-Mijares (176_CR25) 2013; 141 DN Obanda (176_CR6) 2016; 6 MN Hall (176_CR19) 2017; 1556 Y Mizuno (176_CR21) 2010; 24 176_CR23 JM Santos (176_CR27) 2014; 32 C Bañuls (176_CR26) 2016; 35 Y Kido (176_CR1) 2000; 105 K Yoshioka (176_CR9) 1996; 1289 H Funabashi (176_CR15) 2012; 80 A Bertuzzi (176_CR5) 2016; 11 Y Bernardinelli (176_CR11) 2004; 101 SW Ball (176_CR10) 2002; 80 T Sasaki (176_CR7) 2008; 76 27149630 - PLoS One. 2016 May 05;11(5):e0154415 28245809 - BMC Cell Biol. 2017 Feb 28;18(1):15 25529429 - Cell Biochem Funct. 2014 Dec;32(8):621-4 26986810 - Regen Med. 2016 Apr;11(3):245-60 27525648 - Diabetologia. 2016 Nov;59(11):2426-2434 28253982 - Prog Mol Biol Transl Sci. 2017;146:1-11 17406637 - Nat Protoc. 2007;2(3):753-62 26916435 - Sci Rep. 2016 Feb 26;6:22222 25325535 - Int J Mol Sci. 2014 Oct 16;15(10 ):18677-92 18673383 - Differentiation. 2008 Dec;76(10):1023-30 27547541 - PeerJ. 2016 Jul 19;4:e2231 21461712 - Stem Cell Rev. 2011 Nov;7(4):948-57 20028942 - Diabetes. 2010 Mar;59(3):588-99 10642598 - J Clin Invest. 2000 Jan;105(2):199-205 16284704 - Mol Imaging Biol. 2005 Nov-Dec;7(6):388-92 26051494 - Clin Nutr. 2016 Jun;35(3):600-7 28247352 - Methods Mol Biol. 2017;1556:223-236 25134817 - In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):973-81 27396242 - J Diabetes Complications. 2016 Sep-Oct;30(7):1209-16 15466714 - Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14937-42 23790912 - Food Chem. 2013 Nov 15;141(2):1267-72 8605231 - Biochim Biophys Acta. 1996 Feb 9;1289(1):5-9 28247353 - Methods Mol Biol. 2017;1556:237-244 11991231 - Can J Physiol Pharmacol. 2002 Mar;80(3):205-9 28108607 - Diabetes. 2017 Feb;66(2):287-299 20181939 - FASEB J. 2010 Jul;24(7):2245-53 |
| References_xml | – volume: 6 start-page: 22222 year: 2016 ident: CR6 article-title: An extract of L. mitigates obesity induced insulin resistance in mice skeletal muscle via protein phosphatase 2A (PP2A) publication-title: Sci Rep. doi: 10.1038/srep22222 – volume: 101 start-page: 14937 year: 2004 end-page: 14942 ident: CR11 article-title: Astrocytes generate Na -mediated metabolic waves publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.0405315101 – volume: 146 start-page: 1 year: 2017 end-page: 11 ident: CR17 article-title: Clinical aspects of glucose metabolism and chronic disease publication-title: Prog Mol Biol Transl Sci. doi: 10.1016/bs.pmbts.2016.12.011 – volume: 59 start-page: 2426 year: 2016 end-page: 34 ident: CR3 article-title: Anagliptin increases insulin-induced skeletal muscle glucose uptake via an NO-dependent mechanism in mice publication-title: Diabetologia. doi: 10.1007/s00125-016-4071-8 – volume: 49 start-page: 6867 year: 2008 end-page: 6878 ident: CR14 article-title: Synthesis of 2-NBDLG, a fluorescent derivative of -glucosamine; the antipode of -glucose tracer 2-NBDG publication-title: Tetrahedron Lett – volume: 105 start-page: 199 year: 2000 end-page: 205 ident: CR1 article-title: Tissue-specific insulin resistance in mice with mutations in the insulin receptor, IRS-1, and IRS-2 publication-title: J Clin Invest. doi: 10.1172/JCI7917 – volume: 2 start-page: 753 year: 2007 end-page: 762 ident: CR13 article-title: A real-time method of imaging glucose uptake in single, living mammalian cells publication-title: Nat Prot. doi: 10.1038/nprot.2007.76 – volume: 1556 start-page: 223 year: 2017 end-page: 236 ident: CR22 article-title: Engraftment of FACS isolated muscle stem cells into injured skeletal muscle publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-6771-1_11 – volume: 141 start-page: 1267 year: 2013 end-page: 1272 ident: CR25 article-title: A single acute dose of pinitol from a naturally occurring food ingredient decreases hyperglycemia and circulating insulin levels in healthy subjects publication-title: Food Chem doi: 10.1016/j.foodchem.2013.04.042 – volume: 1289 start-page: 5 year: 1996 end-page: 9 ident: CR9 article-title: A novel fluorescence derivative of glucose applicable to the assessment of glucose uptake activity of publication-title: Biochim Biophys Acta doi: 10.1016/0304-4165(95)00153-0 – volume: 80 start-page: 205 year: 2002 end-page: 209 ident: CR10 article-title: A fluorescent compound for glucose uptake measurements in isolated rat cardiomyocytes publication-title: Can J Physiol Pharmacol doi: 10.1139/y02-043 – volume: 59 start-page: 588 year: 2010 end-page: 599 ident: CR2 article-title: Inhibition of PTP1B restores IRS1-mediated hepatic insulin signaling in IRS2-deficient mice publication-title: Diabetes doi: 10.2337/db09-0796 – volume: 4 start-page: e2231 year: 2016 ident: CR16 article-title: Reconstitution of the complete rupture in musculotendinous junction using skeletal muscle-derived multipotent stem cell sheet-pellets as a “bio-bond” publication-title: Peer J. doi: 10.7717/peerj.2231 – volume: 35 start-page: 600 year: 2016 end-page: 607 ident: CR26 article-title: Chronic consumption of an inositol-enriched carob extract improves postprandial glycaemia and insulin sensitivity in healthy subjects: a randomized controlled trial publication-title: Clin Nutr doi: 10.1016/j.clnu.2015.05.005 – volume: 66 start-page: 287 year: 2017 end-page: 299 ident: CR24 article-title: Insulin-like growth factor binding protein 1 could improve glucose regulation and insulin sensitivity through its RGD domain publication-title: Diabetes doi: 10.2337/db16-0997 – volume: 11 start-page: 245 year: 2016 end-page: 260 ident: CR18 article-title: Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers publication-title: Regen Med. doi: 10.2217/rme-2015-0045 – ident: CR23 – volume: 15 start-page: 18677 year: 2014 end-page: 18692 ident: CR28 article-title: Molecular mechanisms for the regulation of insulin-stimulated glucose uptake by small guanosine triphosphatases in skeletal muscle and adipocytes publication-title: Int J Mol Sci doi: 10.3390/ijms151018677 – volume: 50 start-page: 973 year: 2014 end-page: 981 ident: CR8 article-title: Concentration dependent effects of spermine on apoptosis and consequent generation of multilayer myotube sheets from mouse embryoid bodies in vitro publication-title: In Vitro Dev Biol Anim. doi: 10.1007/s11626-014-9799-1 – volume: 76 start-page: 1023 year: 2008 end-page: 1030 ident: CR7 article-title: Generation of a multi-layer muscle fiber sheet from mouse ES cells by the spermine action at specific timing and concentration publication-title: Differentiation. doi: 10.1111/j.1432-0436.2008.00294.x – volume: 24 start-page: 2245 year: 2010 end-page: 2253 ident: CR21 article-title: Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells publication-title: FASEB J. doi: 10.1096/fj.09-137174 – volume: 7 start-page: 388 year: 2005 end-page: 392 ident: CR12 article-title: Uptake of a fluorescent deoxyglucose analog (2-NBDG) in tumor cells publication-title: Mol Imaging Biol. doi: 10.1007/s11307-005-0011-6 – volume: 7 start-page: 948 year: 2011 end-page: 957 ident: CR20 article-title: Functional myogenic engraftment from mouse iPS cells publication-title: Stem Cell Rev Rep. doi: 10.1007/s12015-011-9258-2 – volume: 32 start-page: 621 year: 2014 end-page: 624 ident: CR27 article-title: The interrelation between aPKC and glucose uptake in the skeletal muscle during contraction and insulin stimulation publication-title: Cell Biochem Funct doi: 10.1002/cbf.3081 – volume: 80 start-page: 299 year: 2012 end-page: 301 ident: CR15 article-title: Utilization of fluorescent glucose analog as a metabolic indicator during differentiation publication-title: Electrochemistry doi: 10.5796/electrochemistry.80.299 – volume: 11 start-page: e0154415 year: 2016 ident: CR5 article-title: Insulin signaling in insulin resistance states and cancer: a modeling analysis publication-title: PLoS One doi: 10.1371/journal.pone.0154415 – volume: 1556 start-page: 237 year: 2017 end-page: 244 ident: CR19 article-title: Transplantation of skeletal muscle stem cells publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-6771-1_12 – volume: 30 start-page: 1209 year: 2016 end-page: 1216 ident: CR4 article-title: Effects of thrombin on insulin signalling and glucose uptake in cultured human myotubes publication-title: J Diabetes Complicat doi: 10.1016/j.jdiacomp.2016.06.014 – volume: 35 start-page: 600 year: 2016 ident: 176_CR26 publication-title: Clin Nutr doi: 10.1016/j.clnu.2015.05.005 – volume: 1289 start-page: 5 year: 1996 ident: 176_CR9 publication-title: Biochim Biophys Acta doi: 10.1016/0304-4165(95)00153-0 – volume: 59 start-page: 588 year: 2010 ident: 176_CR2 publication-title: Diabetes doi: 10.2337/db09-0796 – volume: 49 start-page: 6867 year: 2008 ident: 176_CR14 publication-title: Tetrahedron Lett – volume: 11 start-page: e0154415 year: 2016 ident: 176_CR5 publication-title: PLoS One doi: 10.1371/journal.pone.0154415 – volume: 50 start-page: 973 year: 2014 ident: 176_CR8 publication-title: In Vitro Dev Biol Anim. doi: 10.1007/s11626-014-9799-1 – ident: 176_CR23 doi: 10.1186/s12860-017-0131-2 – volume: 6 start-page: 22222 year: 2016 ident: 176_CR6 publication-title: Sci Rep. doi: 10.1038/srep22222 – volume: 2 start-page: 753 year: 2007 ident: 176_CR13 publication-title: Nat Prot. doi: 10.1038/nprot.2007.76 – volume: 32 start-page: 621 year: 2014 ident: 176_CR27 publication-title: Cell Biochem Funct doi: 10.1002/cbf.3081 – volume: 11 start-page: 245 year: 2016 ident: 176_CR18 publication-title: Regen Med. doi: 10.2217/rme-2015-0045 – volume: 66 start-page: 287 year: 2017 ident: 176_CR24 publication-title: Diabetes doi: 10.2337/db16-0997 – volume: 30 start-page: 1209 year: 2016 ident: 176_CR4 publication-title: J Diabetes Complicat doi: 10.1016/j.jdiacomp.2016.06.014 – volume: 4 start-page: e2231 year: 2016 ident: 176_CR16 publication-title: Peer J. doi: 10.7717/peerj.2231 – volume: 141 start-page: 1267 year: 2013 ident: 176_CR25 publication-title: Food Chem doi: 10.1016/j.foodchem.2013.04.042 – volume: 7 start-page: 388 year: 2005 ident: 176_CR12 publication-title: Mol Imaging Biol. doi: 10.1007/s11307-005-0011-6 – volume: 59 start-page: 2426 year: 2016 ident: 176_CR3 publication-title: Diabetologia. doi: 10.1007/s00125-016-4071-8 – volume: 15 start-page: 18677 year: 2014 ident: 176_CR28 publication-title: Int J Mol Sci doi: 10.3390/ijms151018677 – volume: 1556 start-page: 223 year: 2017 ident: 176_CR22 publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-6771-1_11 – volume: 80 start-page: 299 year: 2012 ident: 176_CR15 publication-title: Electrochemistry doi: 10.5796/electrochemistry.80.299 – volume: 1556 start-page: 237 year: 2017 ident: 176_CR19 publication-title: Methods Mol Biol doi: 10.1007/978-1-4939-6771-1_12 – volume: 105 start-page: 199 year: 2000 ident: 176_CR1 publication-title: J Clin Invest. doi: 10.1172/JCI7917 – volume: 101 start-page: 14937 year: 2004 ident: 176_CR11 publication-title: Proc Natl Acad Sci USA doi: 10.1073/pnas.0405315101 – volume: 7 start-page: 948 year: 2011 ident: 176_CR20 publication-title: Stem Cell Rev Rep. doi: 10.1007/s12015-011-9258-2 – volume: 24 start-page: 2245 year: 2010 ident: 176_CR21 publication-title: FASEB J. doi: 10.1096/fj.09-137174 – volume: 76 start-page: 1023 year: 2008 ident: 176_CR7 publication-title: Differentiation. doi: 10.1111/j.1432-0436.2008.00294.x – volume: 146 start-page: 1 year: 2017 ident: 176_CR17 publication-title: Prog Mol Biol Transl Sci. doi: 10.1016/bs.pmbts.2016.12.011 – volume: 80 start-page: 205 year: 2002 ident: 176_CR10 publication-title: Can J Physiol Pharmacol doi: 10.1139/y02-043 – reference: 27525648 - Diabetologia. 2016 Nov;59(11):2426-2434 – reference: 26986810 - Regen Med. 2016 Apr;11(3):245-60 – reference: 28253982 - Prog Mol Biol Transl Sci. 2017;146:1-11 – reference: 11991231 - Can J Physiol Pharmacol. 2002 Mar;80(3):205-9 – reference: 18673383 - Differentiation. 2008 Dec;76(10):1023-30 – reference: 21461712 - Stem Cell Rev. 2011 Nov;7(4):948-57 – reference: 25529429 - Cell Biochem Funct. 2014 Dec;32(8):621-4 – reference: 26916435 - Sci Rep. 2016 Feb 26;6:22222 – reference: 26051494 - Clin Nutr. 2016 Jun;35(3):600-7 – reference: 20181939 - FASEB J. 2010 Jul;24(7):2245-53 – reference: 17406637 - Nat Protoc. 2007;2(3):753-62 – reference: 20028942 - Diabetes. 2010 Mar;59(3):588-99 – reference: 28247352 - Methods Mol Biol. 2017;1556:223-236 – reference: 28108607 - Diabetes. 2017 Feb;66(2):287-299 – reference: 28247353 - Methods Mol Biol. 2017;1556:237-244 – reference: 16284704 - Mol Imaging Biol. 2005 Nov-Dec;7(6):388-92 – reference: 27396242 - J Diabetes Complications. 2016 Sep-Oct;30(7):1209-16 – reference: 25134817 - In Vitro Cell Dev Biol Anim. 2014 Dec;50(10):973-81 – reference: 25325535 - Int J Mol Sci. 2014 Oct 16;15(10 ):18677-92 – reference: 27149630 - PLoS One. 2016 May 05;11(5):e0154415 – reference: 8605231 - Biochim Biophys Acta. 1996 Feb 9;1289(1):5-9 – reference: 27547541 - PeerJ. 2016 Jul 19;4:e2231 – reference: 23790912 - Food Chem. 2013 Nov 15;141(2):1267-72 – reference: 10642598 - J Clin Invest. 2000 Jan;105(2):199-205 – reference: 28245809 - BMC Cell Biol. 2017 Feb 28;18(1):15 – reference: 15466714 - Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14937-42 |
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| SubjectTerms | Animals Biomedical and Life Sciences Cell Biology Cell Differentiation - drug effects Cells, Cultured Confocal microscopy Embryo cells Embryoid Bodies - cytology Embryonic Stem Cells - cytology Glucose Glucose - metabolism Gynecology Insulin Insulin - pharmacology Life Sciences Mice Muscle, Skeletal - cytology Muscle, Skeletal - metabolism Musculoskeletal system Oncology Rapid Communication Reproductive Medicine Rodents Skeletal muscle Skeleton Spermine Spermine - pharmacology Stem cell transplantation Stem Cells Surgery |
| Title | In vitro production of insulin-responsive skeletal muscle tissue from mouse embryonic stem cells by spermine-induced differentiation method |
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