Circulating Endothelial Progenitor Cells Present an Inflammatory Phenotype and Function in Patients With Alcoholic Liver Cirrhosis
Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively...
Saved in:
Published in | Frontiers in physiology Vol. 9; p. 556 |
---|---|
Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
22.05.2018
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively characterized the phenotype and functions of EPCs to understand their role in ALC pathogenesis.
Circulating EPCs were identified as CD34+CD133+CD31+ cells by flow cytometer in ALC patients (
= 7) and healthy controls (HC,
= 7). A comprehensive characterization of circulating EPCs using more than 30 phenotype markers was performed by mass cytometer time of flight (CyTOF) in an independent cohort of age and gender matched ALC patients (
= 4) and controls (
= 5).
cultures of circulating EPCs from ALC patients (
= 20) and controls (
= 18) were also tested for their functions, including colony formation, LDL uptake, lectin binding and cytokine secretion (ELISA).
Three distinct populations of circulating EPCs (CD34+CD133+CD31+) were identified, classified on their CD45 expression (negative: CD45
; intermediate: CD45
; high: CD45
). CD45
and CD45
EPCs significantly increased in ALC patients compared to controls (
-val = 0.006). CyTOF data showed that CD45
EPCs were distinct from CD45
and CD45
EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, and chemokine receptors, CCR2, CCR5, CCR7, and CX3CR1. Similar to circulating EPCs, percentage of CD45
CD34+CD31+ EPCs in
cultures from patients, were significantly higher compared to controls (
< 0.05). Cultured EPCs from patients also showed increased LDL uptake, lectin binding and release of TNF-alpha, RANTES, FGF-2, and VEGF.
We report the first extensive characterization of circulating human EPCs with distinct EPC subtypes. Increase in CD45
EPC subtype in ALC patients with enhanced functions, inflammatory cytokines and angiogenic mediators in patients suggests an inflammatory role for these cells in ALC. |
---|---|
AbstractList | Background and Aim:
Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively characterized the phenotype and functions of EPCs to understand their role in ALC pathogenesis.
Methods:
Circulating EPCs were identified as CD34+CD133+CD31+ cells by flow cytometer in ALC patients (
n
= 7) and healthy controls (HC,
n
= 7). A comprehensive characterization of circulating EPCs using more than 30 phenotype markers was performed by mass cytometer time of flight (CyTOF) in an independent cohort of age and gender matched ALC patients (
n
= 4) and controls (
n
= 5).
Ex vivo
cultures of circulating EPCs from ALC patients (
n
= 20) and controls (
n
= 18) were also tested for their functions, including colony formation, LDL uptake, lectin binding and cytokine secretion (ELISA).
Results:
Three distinct populations of circulating EPCs (CD34+CD133+CD31+) were identified, classified on their CD45 expression (negative: CD45
−
; intermediate: CD45
int
; high: CD45
hi
). CD45
int
and CD45
hi
EPCs significantly increased in ALC patients compared to controls (
p
-val = 0.006). CyTOF data showed that CD45
hi
EPCs were distinct from CD45
−
and CD45
int
EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, and chemokine receptors, CCR2, CCR5, CCR7, and CX3CR1. Similar to circulating EPCs, percentage of CD45
hi
CD34+CD31+ EPCs in
ex-vivo
cultures from patients, were significantly higher compared to controls (
p
< 0.05). Cultured EPCs from patients also showed increased LDL uptake, lectin binding and release of TNF-alpha, RANTES, FGF-2, and VEGF.
Conclusions:
We report the first extensive characterization of circulating human EPCs with distinct EPC subtypes. Increase in CD45
hi
EPC subtype in ALC patients with enhanced functions, inflammatory cytokines and angiogenic mediators in patients suggests an inflammatory role for these cells in ALC. Background and Aim: Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively characterized the phenotype and functions of EPCs to understand their role in ALC pathogenesis. Methods: Circulating EPCs were identified as CD34+CD133+CD31+ cells by flow cytometer in ALC patients (n = 7) and healthy controls (HC, n = 7). A comprehensive characterization of circulating EPCs using more than 30 phenotype markers was performed by mass cytometer time of flight (CyTOF) in an independent cohort of age and gender matched ALC patients (n = 4) and controls (n = 5). Ex vivo cultures of circulating EPCs from ALC patients (n = 20) and controls (n = 18) were also tested for their functions, including colony formation, LDL uptake, lectin binding and cytokine secretion (ELISA). Results: Three distinct populations of circulating EPCs (CD34+CD133+CD31+) were identified, classified on their CD45 expression (negative: CD45-; intermediate: CD45int; high: CD45hi). CD45int and CD45hi EPCs significantly increased in ALC patients compared to controls (p-val = 0.006). CyTOF data showed that CD45hi EPCs were distinct from CD45- and CD45int EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, and chemokine receptors, CCR2, CCR5, CCR7, and CX3CR1. Similar to circulating EPCs, percentage of CD45hiCD34+CD31+ EPCs in ex-vivo cultures from patients, were significantly higher compared to controls (p < 0.05). Cultured EPCs from patients also showed increased LDL uptake, lectin binding and release of TNF-alpha, RANTES, FGF-2, and VEGF. Conclusions: We report the first extensive characterization of circulating human EPCs with distinct EPC subtypes. Increase in CD45hi EPC subtype in ALC patients with enhanced functions, inflammatory cytokines and angiogenic mediators in patients suggests an inflammatory role for these cells in ALC. Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively characterized the phenotype and functions of EPCs to understand their role in ALC pathogenesis. Circulating EPCs were identified as CD34+CD133+CD31+ cells by flow cytometer in ALC patients ( = 7) and healthy controls (HC, = 7). A comprehensive characterization of circulating EPCs using more than 30 phenotype markers was performed by mass cytometer time of flight (CyTOF) in an independent cohort of age and gender matched ALC patients ( = 4) and controls ( = 5). cultures of circulating EPCs from ALC patients ( = 20) and controls ( = 18) were also tested for their functions, including colony formation, LDL uptake, lectin binding and cytokine secretion (ELISA). Three distinct populations of circulating EPCs (CD34+CD133+CD31+) were identified, classified on their CD45 expression (negative: CD45 ; intermediate: CD45 ; high: CD45 ). CD45 and CD45 EPCs significantly increased in ALC patients compared to controls ( -val = 0.006). CyTOF data showed that CD45 EPCs were distinct from CD45 and CD45 EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, and chemokine receptors, CCR2, CCR5, CCR7, and CX3CR1. Similar to circulating EPCs, percentage of CD45 CD34+CD31+ EPCs in cultures from patients, were significantly higher compared to controls ( < 0.05). Cultured EPCs from patients also showed increased LDL uptake, lectin binding and release of TNF-alpha, RANTES, FGF-2, and VEGF. We report the first extensive characterization of circulating human EPCs with distinct EPC subtypes. Increase in CD45 EPC subtype in ALC patients with enhanced functions, inflammatory cytokines and angiogenic mediators in patients suggests an inflammatory role for these cells in ALC. Background and Aim: Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain elusive. In particular, their involvement in the pathogenesis of alcoholic liver cirrhosis (ALC) remains unclear. The current study extensively characterized the phenotype and functions of EPCs to understand their role in ALC pathogenesis.Methods: Circulating EPCs were identified as CD34+CD133+CD31+ cells by flow cytometer in ALC patients (n = 7) and healthy controls (HC, n = 7). A comprehensive characterization of circulating EPCs using more than 30 phenotype markers was performed by mass cytometer time of flight (CyTOF) in an independent cohort of age and gender matched ALC patients (n = 4) and controls (n = 5). Ex vivo cultures of circulating EPCs from ALC patients (n = 20) and controls (n = 18) were also tested for their functions, including colony formation, LDL uptake, lectin binding and cytokine secretion (ELISA).Results: Three distinct populations of circulating EPCs (CD34+CD133+CD31+) were identified, classified on their CD45 expression (negative: CD45−; intermediate: CD45int; high: CD45hi). CD45int and CD45hi EPCs significantly increased in ALC patients compared to controls (p-val = 0.006). CyTOF data showed that CD45hi EPCs were distinct from CD45− and CD45int EPCs, with higher expression of T cell and myeloid markers, including CD3, CD4, HLA-DR, and chemokine receptors, CCR2, CCR5, CCR7, and CX3CR1. Similar to circulating EPCs, percentage of CD45hiCD34+CD31+ EPCs in ex-vivo cultures from patients, were significantly higher compared to controls (p < 0.05). Cultured EPCs from patients also showed increased LDL uptake, lectin binding and release of TNF-alpha, RANTES, FGF-2, and VEGF.Conclusions: We report the first extensive characterization of circulating human EPCs with distinct EPC subtypes. Increase in CD45hi EPC subtype in ALC patients with enhanced functions, inflammatory cytokines and angiogenic mediators in patients suggests an inflammatory role for these cells in ALC. |
Author | Shastry, Saggere M Sarin, Shiv Kaur, Savneet Fazekas St de Groth, Barbara Seth, Devanshi Sehgal, Rashi McGuire, Helen M McCaughan, Geoffrey Trehanpati, Nirupma |
AuthorAffiliation | 2 Liver Injury and Cancer, Centenary Institute of Cancer Medicine and Cell Biology , Camperdown, NSW , Australia 7 Faculty of Medicine, The University of Sydney , Sydney, NSW , Australia 4 Ramaciotti Facility for Human Systems Biology, University of Sydney , Sydney, NSW , Australia 6 Drug Health Services, Royal Prince Alfred Hospital , Camperdown, NSW , Australia 1 Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences , New Delhi , India 3 Sydney Medical School, University of Sydney , Sydney, NSW , Australia 5 Discipline of Pathology, University of Sydney , Sydney, NSW , Australia |
AuthorAffiliation_xml | – name: 5 Discipline of Pathology, University of Sydney , Sydney, NSW , Australia – name: 1 Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences , New Delhi , India – name: 3 Sydney Medical School, University of Sydney , Sydney, NSW , Australia – name: 4 Ramaciotti Facility for Human Systems Biology, University of Sydney , Sydney, NSW , Australia – name: 7 Faculty of Medicine, The University of Sydney , Sydney, NSW , Australia – name: 6 Drug Health Services, Royal Prince Alfred Hospital , Camperdown, NSW , Australia – name: 2 Liver Injury and Cancer, Centenary Institute of Cancer Medicine and Cell Biology , Camperdown, NSW , Australia |
Author_xml | – sequence: 1 givenname: Savneet surname: Kaur fullname: Kaur, Savneet organization: Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India – sequence: 2 givenname: Rashi surname: Sehgal fullname: Sehgal, Rashi organization: Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India – sequence: 3 givenname: Saggere M surname: Shastry fullname: Shastry, Saggere M organization: Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India – sequence: 4 givenname: Geoffrey surname: McCaughan fullname: McCaughan, Geoffrey organization: Liver Injury and Cancer, Centenary Institute of Cancer Medicine and Cell Biology, Camperdown, NSW, Australia – sequence: 5 givenname: Helen M surname: McGuire fullname: McGuire, Helen M organization: Ramaciotti Facility for Human Systems Biology, University of Sydney, Sydney, NSW, Australia – sequence: 6 givenname: Barbara surname: Fazekas St de Groth fullname: Fazekas St de Groth, Barbara organization: Discipline of Pathology, University of Sydney, Sydney, NSW, Australia – sequence: 7 givenname: Shiv surname: Sarin fullname: Sarin, Shiv organization: Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India – sequence: 8 givenname: Nirupma surname: Trehanpati fullname: Trehanpati, Nirupma organization: Department of Molecular and Cellular Medicine, Institute of Liver and Biliary Sciences, New Delhi, India – sequence: 9 givenname: Devanshi surname: Seth fullname: Seth, Devanshi organization: Faculty of Medicine, The University of Sydney, Sydney, NSW, Australia |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/29872403$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkk1vGyEQhlGVqEnd3HuqOPZiF1j261IpspLWkqX40Kq9oVl21kuEwQU2kq_55SV2GiVcQO_MPDPA-4GcOe-QkE-cLYqiab8O-_EQF4LxZsFYWVbvyCWvKjlnUvw5e3W-IFcx3rO8JBOM8ffkQrRNLSQrLsnj0gQ9WUjGbemN630a0RqwdBP8Fp1JPtAlWhuzgBFdouDoyg0WdjvIwQPdjOh8OuwxR3p6OzmdjHfUOLrJ1FwR6W-TRnpttR-9NZquzQNmqglh9NHEj-R8ABvx6nmfkV-3Nz-XP-bru--r5fV6rmUl0lyXstEdYMF414PmCFpXvKmgHCqB0GmhC1GzQvASatF1Xd1kFWUxwNBWXV_MyOrE7T3cq30wOwgH5cGoo-DDVkFIRltUDaDogPU4DLVssIVe972sBWvLthlykxn5dmLtp26Hvc7XDGDfQN9GnBnV1j-osq2FaIoM-PIMCP7vhDGpnYk6PzQ49FNUgpWcVW0rRU5lp1QdfIwBh5c2nKknJ6ijE9STE9TRCbnk8-vxXgr-_3vxDxyStzs |
CitedBy_id | crossref_primary_10_3892_wasj_2022_158 crossref_primary_10_1161_JAHA_119_014143 crossref_primary_10_1177_2041731420986711 crossref_primary_10_1177_1358863X221126205 crossref_primary_10_3390_cells9010107 crossref_primary_10_1016_j_chemosphere_2019_125077 crossref_primary_10_3390_jcm8111984 crossref_primary_10_1007_s12195_023_00771_1 crossref_primary_10_3389_fimmu_2020_01481 crossref_primary_10_3390_cancers14133088 crossref_primary_10_1038_s41598_019_40612_8 crossref_primary_10_1111_acer_14242 crossref_primary_10_3390_cells8111339 crossref_primary_10_1002_hep_31179 |
Cites_doi | 10.1126/science.275.5302.964 10.1182/blood-2006-08-043471 10.3109/15376516.2012.666651 10.1038/s41698-017-0006-1 10.1161/01.RES.0000193596.94936.2c 10.1101/cshperspect.a006692 10.1016/j.jcmgh.2015.06.011 10.1002/cyto.a.22730 10.1155/2014/712893 10.1016/j.exphem.2007.04.002 10.1155/2011/846096 10.1111/cpr.12355 10.1152/ajpheart.00665.2008 10.3748/wjg.v20.i26.8393 10.1016/j.immuni.2012.01.002 10.1002/stem.745 10.1186/1755-8794-3-18 10.1016/j.jhep.2012.07.016 10.1126/science.1198704 10.1172/JCI66025 10.1007/s12072-013-9483-7 10.1172/JCI43027 10.1111/j.1582-4934.2008.00598.x 10.2119/molmed.2009.00032 10.1056/NEJMoa022287 10.1093/cvr/cvv111 10.1161/01.ATV.0000114236.77009.06 10.1182/blood-2004-04-1396 10.1111/j.1530-0277.2009.01060.x |
ContentType | Journal Article |
Copyright | Copyright © 2018 Kaur, Sehgal, Shastry, McCaughan, McGuire, Fazekas St de Groth, Sarin, Trehanpati and Seth. 2018 Kaur, Sehgal, Shastry, McCaughan, McGuire, Fazekas St de Groth, Sarin, Trehanpati and Seth |
Copyright_xml | – notice: Copyright © 2018 Kaur, Sehgal, Shastry, McCaughan, McGuire, Fazekas St de Groth, Sarin, Trehanpati and Seth. 2018 Kaur, Sehgal, Shastry, McCaughan, McGuire, Fazekas St de Groth, Sarin, Trehanpati and Seth |
DBID | NPM AAYXX CITATION 7X8 5PM DOA |
DOI | 10.3389/fphys.2018.00556 |
DatabaseName | PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE - Academic PubMed |
Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
EISSN | 1664-042X |
EndPage | 556 |
ExternalDocumentID | oai_doaj_org_article_8ae2ba0deff748e9adcdd47209598f70 10_3389_fphys_2018_00556 29872403 |
Genre | Journal Article |
GrantInformation_xml | – fundername: Australia-India Strategic Research Fund grantid: AISRF07440; BT/Indo-Aus/07/12/2013 |
GroupedDBID | 53G 5VS 9T4 AAFWJ AAKDD ACGFO ACGFS ACXDI ADBBV ADRAZ AENEX AFPKN ALMA_UNASSIGNED_HOLDINGS AOIJS BCNDV DIK EMOBN F5P GROUPED_DOAJ GX1 HYE IAO IEA IHR IHW IPNFZ ISR KQ8 M48 M~E NPM O5R O5S OK1 PGMZT RIG RNS RPM AAYXX CITATION 7X8 ITC 5PM |
ID | FETCH-LOGICAL-c462t-c548cbae301bdac1eacc6186a5f62eabc2c32703215a72bbb78eabe43faf96bd3 |
IEDL.DBID | RPM |
ISSN | 1664-042X |
IngestDate | Fri Oct 04 13:08:51 EDT 2024 Tue Sep 17 21:29:16 EDT 2024 Sat Aug 17 05:49:49 EDT 2024 Thu Sep 26 18:27:20 EDT 2024 Sat Sep 28 08:06:46 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | alcoholic liver cirrhosis inflammation angiogenesis endothelial progenitor cells CD34 CD45 mass cytometry CyTOF CD133 |
Language | English |
License | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c462t-c548cbae301bdac1eacc6186a5f62eabc2c32703215a72bbb78eabe43faf96bd3 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Gastrointestinal Sciences, a section of the journal Frontiers in Physiology Edited by: Atsushi Masamune, Tohoku University, Japan These authors have contributed equally to this work. Reviewed by: Takayuki Kogure, Tohoku Medical and Pharmaceutical University, Japan; Michael Hickey, Monash University, Australia |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972283/ |
PMID | 29872403 |
PQID | 2051069942 |
PQPubID | 23479 |
PageCount | 1 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_8ae2ba0deff748e9adcdd47209598f70 pubmedcentral_primary_oai_pubmedcentral_nih_gov_5972283 proquest_miscellaneous_2051069942 crossref_primary_10_3389_fphys_2018_00556 pubmed_primary_29872403 |
PublicationCentury | 2000 |
PublicationDate | 2018-05-22 |
PublicationDateYYYYMMDD | 2018-05-22 |
PublicationDate_xml | – month: 05 year: 2018 text: 2018-05-22 day: 22 |
PublicationDecade | 2010 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland |
PublicationTitle | Frontiers in physiology |
PublicationTitleAlternate | Front Physiol |
PublicationYear | 2018 |
Publisher | Frontiers Media S.A |
Publisher_xml | – name: Frontiers Media S.A |
References | Bocca (B5) 2015; 1 Raskopf (B21) 2014; 2014 Hur (B12) 2004; 24 Walter (B26) 2005; 97 Hill (B11) 2003; 348 Kaur (B15) 2013; 7 Loomans (B18) 2009; 15 Timmermans (B24) 2009; 13 Zhang (B31) 2009; 296 Bendall (B4) 2011; 332 Tanaka (B23) 2017; 1 Herlea-Pana (B10) 2015; 106 DeLeve (B8) 2013; 123 Newell (B20) 2012; 36 Kaur (B16) 2012; 57 Toshikuni (B25) 2014; 20 (B28) 2014 Ingram (B13) 2004; 104 Asahara (B3) 1997; 275 Ishida (B14) 2012; 122 Das (B7) 2012; 22 Medina (B19) 2010; 3 Asahara (B2) 2011; 29 Case (B6) 2007; 35 B1 Yoder (B29) 2012; 2 Yoder (B30) 2007; 109 Garg (B9) 2017; 50 Seth (B22) 2010; 34 Wang (B27) 2011; 2011 Lanuti (B17) 2016; 89 |
References_xml | – volume: 275 start-page: 964 year: 1997 ident: B3 article-title: Isolation of putative progenitor endothelial cells for angiogenesis publication-title: Science doi: 10.1126/science.275.5302.964 contributor: fullname: Asahara – volume: 109 start-page: 1801 year: 2007 ident: B30 article-title: Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals publication-title: Blood doi: 10.1182/blood-2006-08-043471 contributor: fullname: Yoder – volume: 22 start-page: 375 year: 2012 ident: B7 article-title: Effects of long term ethanol consumption mediated oxidative stress on neovessel generation in liver publication-title: Toxicol. Mech. Methods doi: 10.3109/15376516.2012.666651 contributor: fullname: Das – volume: 1 start-page: 6 year: 2017 ident: B23 article-title: The lack of increases in circulating endothelial progenitor cell as a negative predictor for pathological response to neoadjuvant chemotherapy in breast cancer patients publication-title: NPJ Precis. Oncol. doi: 10.1038/s41698-017-0006-1 contributor: fullname: Tanaka – volume: 97 start-page: 1142 year: 2005 ident: B26 article-title: Impaired CXCR4 signaling contributes to the reduced neovascularization capacity of endothelial progenitor cells from patients with coronary artery disease publication-title: Circ. Res. doi: 10.1161/01.RES.0000193596.94936.2c contributor: fullname: Walter – volume: 2 start-page: a006692 year: 2012 ident: B29 article-title: Human endothelial progenitor cells publication-title: Cold Spring Harb. Perspect. Med. doi: 10.1101/cshperspect.a006692 contributor: fullname: Yoder – volume: 1 start-page: 477 year: 2015 ident: B5 article-title: Angiogenesis and fibrogenesis in chronic liver diseases publication-title: Cell. Mol. Gastroenterol. Hepatol doi: 10.1016/j.jcmgh.2015.06.011 contributor: fullname: Bocca – volume: 89 start-page: 259 year: 2016 ident: B17 article-title: Endothelial progenitor cells, defined by the simultaneous surface expression of VEGFR2 and CD133, are not detectable in healthy peripheral and cord blood publication-title: Cytometry Part A doi: 10.1002/cyto.a.22730 contributor: fullname: Lanuti – ident: B1 – volume: 2014 start-page: 712893 year: 2014 ident: B21 article-title: Toxic damage increases angiogenesis and metastasis in fibrotic livers via PECAM-1 publication-title: Biomed Res. Int. doi: 10.1155/2014/712893 contributor: fullname: Raskopf – volume: 35 start-page: 1109 year: 2007 ident: B6 article-title: Human CD34+AC133+VEGFR-2+ cells are not endothelial progenitor cells but distinct, primitive hematopoietic progenitors publication-title: Exp. Hematol. doi: 10.1016/j.exphem.2007.04.002 contributor: fullname: Case – volume: 2011 start-page: 846096 year: 2011 ident: B27 article-title: An in vitro study of differentiation of hematopoietic cells to endothelial cells publication-title: Bone Marrow Res. doi: 10.1155/2011/846096 contributor: fullname: Wang – volume: 50 start-page: e12355 year: 2017 ident: B9 article-title: Bone marrow endothelial progenitor cells activate hepatic stellate cells and aggravate carbon tetrachloride induced liver fibrosis in mice via paracrine factors publication-title: Cell Prolif. doi: 10.1111/cpr.12355 contributor: fullname: Garg – volume: 296 start-page: H1675 year: 2009 ident: B31 article-title: Release of proinflammatory mediators and expression of proinflammatory adhesion molecules by endothelial progenitor cells publication-title: Am. J. Physiol. Heart Circ. Physiol. doi: 10.1152/ajpheart.00665.2008 contributor: fullname: Zhang – volume: 20 start-page: 8393 year: 2014 ident: B25 article-title: Clinical differences between alcoholic liver disease and nonalcoholic fatty liver disease publication-title: World J. Gastroenterol. doi: 10.3748/wjg.v20.i26.8393 contributor: fullname: Toshikuni – volume: 36 start-page: 142 year: 2012 ident: B20 article-title: Cytometry by time-of-flight shows combinatorial cytokine expression and virus-specific cell niches within a continuum of CD8(+) T cell phenotypes publication-title: Immunity doi: 10.1016/j.immuni.2012.01.002 contributor: fullname: Newell – volume: 29 start-page: 1650 year: 2011 ident: B2 article-title: Concise review: circulating endothelial progenitor cells for vascular medicine publication-title: Stem Cells doi: 10.1002/stem.745 contributor: fullname: Asahara – volume: 3 start-page: 18 year: 2010 ident: B19 article-title: Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities publication-title: BMC Med. Genomics doi: 10.1186/1755-8794-3-18 contributor: fullname: Medina – volume: 57 start-page: 1193 year: 2012 ident: B16 article-title: Increased number and function of endothelial progenitor cells stimulate angiogenesis by resident liver sinusoidal endothelial cells (SECs) in cirrhosis through paracrine factors publication-title: J. Hepatol. doi: 10.1016/j.jhep.2012.07.016 contributor: fullname: Kaur – volume: 332 start-page: 687 year: 2011 ident: B4 article-title: Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum publication-title: Science doi: 10.1126/science.1198704 contributor: fullname: Bendall – volume: 123 start-page: 1861 year: 2013 ident: B8 article-title: Liver sinusoidal endothelial cells and liver regeneration publication-title: J. Clin. Invest. doi: 10.1172/JCI66025 contributor: fullname: DeLeve – volume: 7 start-page: 959 year: 2013 ident: B15 article-title: Angiogenesis in liver regeneration and fibrosis: “a double-edged sword” publication-title: Hepatol. Int. doi: 10.1007/s12072-013-9483-7 contributor: fullname: Kaur – volume: 122 start-page: 711 year: 2012 ident: B14 article-title: Pivotal role of the CCL5/CCR5 interaction for recruitment of endothelial progenitor cells in mouse wound healing publication-title: J. Clin. Invest. doi: 10.1172/JCI43027 contributor: fullname: Ishida – volume: 13 start-page: 87 year: 2009 ident: B24 article-title: Endothelial progenitor cells: identity defined? publication-title: J. Cell. Mol. Med. doi: 10.1111/j.1582-4934.2008.00598.x contributor: fullname: Timmermans – volume: 15 start-page: 152 year: 2009 ident: B18 article-title: Differentiation of bone marrow-derived endothelial progenitor cells is shifted into a proinflammatory phenotype by hyperglycemia publication-title: Mol. Med. doi: 10.2119/molmed.2009.00032 contributor: fullname: Loomans – volume: 348 start-page: 593 year: 2003 ident: B11 article-title: Circulating endothelial progenitor cells, vascular function, and cardiovascular risk publication-title: N. Engl. J. Med. doi: 10.1056/NEJMoa022287 contributor: fullname: Hill – volume: 106 start-page: 324 year: 2015 ident: B10 article-title: Chemokine receptors CXCR2 and CX3CR1 differentially regulate functional responses of bone-marrow endothelial progenitors during atherosclerotic plaque regression publication-title: Cardiovasc. Res. doi: 10.1093/cvr/cvv111 contributor: fullname: Herlea-Pana – volume: 24 start-page: 288 year: 2004 ident: B12 article-title: Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis publication-title: Arterioscler. Thromb. Vasc. Biol. doi: 10.1161/01.ATV.0000114236.77009.06 contributor: fullname: Hur – volume: 104 start-page: 2752 year: 2004 ident: B13 article-title: Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood publication-title: Blood doi: 10.1182/blood-2004-04-1396 contributor: fullname: Ingram – volume: 34 start-page: 4 year: 2010 ident: B22 article-title: Alcohol, signaling, and ECM turnover publication-title: Alcohol. Clin. Exp. Res. doi: 10.1111/j.1530-0277.2009.01060.x contributor: fullname: Seth – volume-title: Global Status Report on Alcohol and Health 2014. year: 2014 ident: B28 |
SSID | ssj0000402001 |
Score | 2.2990701 |
Snippet | Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these heterogenous EPCs remain... Background and Aim: Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these... Background and Aim: Endothelial progenitor cells (EPCs) have been implicated in liver injury and repair. However, the phenotype and potential of these... |
SourceID | doaj pubmedcentral proquest crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | 556 |
SubjectTerms | alcoholic liver cirrhosis angiogenesis CD45 endothelial progenitor cells inflammation mass cytometry CyTOF Physiology |
SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LSwMxEA7iyYsovuqLCF48LG6zzxy1WFRUelD0tuRpF2pWtu0f8Jc7k7SlFcGL183uJuSbzCOZfEPIea5SwXSso0IzEaVgISMelzKypTSJjrno-pT_x6f89iW9f8velkp9YU5YoAcOE3dZCsOkiLWxtkhLw4VWWsM_cf-qtEWI1rvZUjDldTCGRXE3nEtCFMYvLe4UYCoX5k5mWK96yQ55uv7ffMyfqZJLtqe_RTZnTiO9CoPdJmvG7ZCvXt0qX3zLvdMbp_Eq1QikiQ7aBqQCVmpLe2Y0GtNBuGJEhaN3zoIIfPijdToYGtfgHiy0aNoHC4co0drRQWBbHdPXejKkV6GMbq3oA2ZxUOi4HTbjerxLXvo3z73baFZRIVJpziaRgvhESWFgVUstVBe0rkLCfJHZnBkhFVMJAx0AfoAomJSyKOGpSRMrLM-lTvbIumucOSBU84QraRjXhUh1xqXMSviCGQzxEss65GI-v9VnIM6oIOBALCqPRYVYVB6LDrlGABbvIeW1fwCCUM0EofpLEDrkbA5fBUsEzz2EM80UOwLFk3OewqD2A5yLrhgvC6Qk7JBiBeiVsay2uHroabghFEPuoMP_GPwR2cDpwLQExo7J-qSdmhPwdiby1Av2N-jPBSQ priority: 102 providerName: Directory of Open Access Journals – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwlV1Lb9QwELZKe-GCQLwWKDISFw5ps47jxAdUlVVXbUXRHrrq3iI_u5EWp022Elz55cw4aWHRXjjGedjxN097PEPIR2G4Yja1SWGZSjhoyESmpU58qV1mU6nGMeT_4ps4nfPzRb74czx6mMBuq2uH9aTm7ergx-3PI2D4z-hxgr499LgIgFFaGBaZ5-IR2WM840jvF4OxH-UyukqxHvJYCIy-YIt-33LrRzb0VEznv80G_TeU8i_dNH1KngxGJT3uqeAZ2XHhOfk1qVsTi3OFa3oSLB61WgG10VnbANUAJ7d04larjs76I0hUBXoWPJDI97j1TmdLFxpco4U7lk5BAyKKtA501mdj7ehVvV7S477Mbm3oV4zyoNBxu2y6untB5tOTy8lpMlRcSAwXbJ0Y8F-MVg64XltlxiCVDSbUV7kXzCltmMkYyAiwE1TBtNZFCa2OZ155KbTNXpLd0AT3mlArM2m0Y9IWittcap2X8AZz6AJmno3Ip_v5rW76xBoVOCSIRRWxqBCLKmIxIl8QgIfnMCV2bGja62rgsKpUjmmVWud9wUsnlTXWAvHhQmfpi3REPtzDVwEL4b6ICq65w45AMAkpOQzqVQ_nQ1dMlgWmLByRYgPojbFs3gn1MqbpBlcNcwu9-Y8ffUse4wVGJzD2juyu2zu3D0bPWr-PtPwbHqcFLQ priority: 102 providerName: Scholars Portal |
Title | Circulating Endothelial Progenitor Cells Present an Inflammatory Phenotype and Function in Patients With Alcoholic Liver Cirrhosis |
URI | https://www.ncbi.nlm.nih.gov/pubmed/29872403 https://search.proquest.com/docview/2051069942 https://pubmed.ncbi.nlm.nih.gov/PMC5972283 https://doaj.org/article/8ae2ba0deff748e9adcdd47209598f70 |
Volume | 9 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT-MwELaAE5fVImC3uwsyEpc9hKaO8_ARKipAdJUDCG6RnzRS66C0_AF-OTNOgyjaE5cc7Fi2_I3nYX8eE3KaaS6ZiU2UGyYjDhYyEnGhIlcom5hYyFGg_E__ZVf3_OYxfdwiaX8XJpD2tarP_Hxx5utZ4FY-L_Sw54kNy-kYnGDM2jLcJtt5knwI0YP6xYgoHnVHkhCAiaHDTQJkcSFtMk3x1SIGoTZmotuwRiFp__88zc-EyQ8WaPKdfFu7jvS8G-Ie2bJ-n7yO61aHJ7j8E730Bi9UzUGmaNk2IBuwXls6tvP5kpbdRSMqPb32DgRhEQ7YaTmzvsGdWKgxdAJ2DrGitadll3N1SR_q1Yyed4_p1preIpeDQsftrFnWywNyP7m8G19F63cVIs0ztoo0RClaSQtrWxmpR6B7NabNl6nLmJVKM50w0ATgDcicKaXyAkotT5x0IlMmOSQ7vvH2J6FGJEIry4TJJTepUCotoAWzGOgljg3I335-q-cufUYFYQfCUgVYKoSlCrAMyAUC8P4fJr4OBU37VK3hrwppmZKxsc7lvLBCGm0MiBhuZxYujwfkpIevgoWCpx_S2-YFOwL1kwnBYVA_Ojjfu-rFYUDyDaA3xrJZA7IZknGvZfHXl1v-Jrs4B8hIYOwP2Vm1L_YIHJ2VOg4bBPCd8uI4CPkbGzsGHQ |
link.rule.ids | 230,315,733,786,790,870,891,2115,24346,27957,27958,53827,53829 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07b9swECbSdGiXPtCXmz5YoEsH2TL15JgaMZzWDjQkbTaBz1ioTQWyvGTsL-8dZQVx0KVdRQmkeB-Pd-THj4R8TlUsmA51kGkmghhmyICHuQxsLk2kQy7GnvK_OEtnF_G3y-TygCT9WRhP2leyGrrVeuiqpedWXq_VqOeJjYrFBIJgVG0ZPSAPYbyy7E6S7h0w5kThuNuUhBSMjywuEyCPC4mTSYL3FjFItlGLbm8-8rL9f4s171Mm78xB06fkR9_6jnrya7ht5VDd3BN2_Offe0ae7KJSetwVPycHxr0gvydVo_ztXu6KnjiNZ7VWAFdaNDXADlxBQydmtdrQojvDRIWjp84CxtZ-754WS-NqXOSFEk2nMIUiDGjlaNHJuW7oz6pd0uPunt5K0TnSRChU3CzrTbV5SS6mJ-eTWbC7siFQccraQEECpKQw4DakFmoMbl2hIr9IbMqMkIqpiIGTgUBDZExKmeXw1MSRFZanUkevyKGrnXlDqOYRV9IwrjMR64RLmeTwBTOYQ0aWDciX3nDldafMUUJGg_Yuvb1LtHfp7T0gX9Gyt--hprZ_UDdX5a7ry1wYJkWojbVZnBsutNIa0IsrpbnNwgH51OOihDGIGyvCmXqLFYFnSzmPoVGvO5zcVtXjbECyPQTttWW_BHDhdb53OHj7319-JI9m54t5OT89-35EHmN_IPGBsXfksG225j3EU6384EfPH-LMJko |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07b9swECbaFCi69IE-4j5ZoEsHWTL15Ji6MZI2CTQ0aNBF4DMWalOGLC8d-8t7R1mBHXTKKoqgpPt4vCM_fUfIp0wlgulIB7lmIkhghQx4VMjAFtLEOuJi4in_5xfZyWXy7Sq92in15Un7StZjt1iOXT333MrVUoUDTywsz6cQBKNqS7jSNrxPHsCcZXwnUfdOGPOiaNIfTEIaxkOLWwXI5ULyZJpi7SIGCTfq0e2tSV66_3_x5m3a5M46NHtCfg1v0NNPfo83nRyrP7fEHe_0ik_J4210So_6W56Re8Y9J3-ndat8lS93TY-dxn-2FgBbWrYNwA9cQkunZrFY07L_l4kKR0-dBawt_Rk-LefGNbjZCy2azmApRTjQ2tGyl3Vd0591N6dHfb3eWtEzpItQGLidN-t6_YJczo5_TE-CbemGQCUZ6wIFiZCSwoD7kFqoCbh3hcr8IrUZM0IqpmIGzgYCDpEzKWVewFWTxFZYnkkdvyQHrnHmkFDNY66kYVznItEplzItoAczmEvGlo3I58F41apX6Kggs0GbV97mFdq88jYfkS9o3Zv7UFvbX2ja62r7-atCGCZFpI21eVIYLrTSGlCMO6aFzaMR-Thgo4K5iAcswplmgwOBh8s4T-ChXvVYuRlqwNqI5Hso2nuW_RbAhtf73mLh9Z17fiAPy6-z6uz04vsb8gg_B_IfGHtLDrp2Y95BWNXJ934C_QPeiSjK |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Circulating+Endothelial+Progenitor+Cells+Present+an+Inflammatory+Phenotype+and+Function+in+Patients+With+Alcoholic+Liver+Cirrhosis&rft.jtitle=Frontiers+in+physiology&rft.au=Kaur%2C+Savneet&rft.au=Sehgal%2C+Rashi&rft.au=Shastry%2C+Saggere+M.&rft.au=McCaughan%2C+Geoffrey&rft.date=2018-05-22&rft.issn=1664-042X&rft.eissn=1664-042X&rft.volume=9&rft_id=info:doi/10.3389%2Ffphys.2018.00556&rft.externalDBID=n%2Fa&rft.externalDocID=10_3389_fphys_2018_00556 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1664-042X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1664-042X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1664-042X&client=summon |