Enzyme immunoassay for the identification of hemoglobin variants

• Published in: Hemoglobin Vol. 14; no. 4; p. 389
• Main Authors: Moscoso, H, Kiefer, C R, Shyamala, M, Garver, F A
• Format: Journal Article
• Language: English
• Published: England 1990
• Subjects: Adult; Animals; Antibody Specificity; Enzyme-Linked Immunosorbent Assay; Hemoglobin J - analysis; Hemoglobin J - immunology; Hemoglobins, Abnormal - analysis; Hemoglobins, Abnormal - immunology; Humans; Immune Sera; Immunoenzyme Techniques; Infant, Newborn; Isoelectric Focusing; Rabbits

We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their identification in hemolysates. Hbs in adult or cord blood hemolysates were coated to the wells of microtiter plates and reacted with the appropriate...