Enzyme immunoassay for the identification of hemoglobin variants
We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their identification in hemolysates. Hbs in adult or cord blood hemolysates were coated to the wells of microtiter plates and reacted with the appropriate...
Saved in:
Published in | Hemoglobin Vol. 14; no. 4; p. 389 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
1990
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their identification in hemolysates. Hbs in adult or cord blood hemolysates were coated to the wells of microtiter plates and reacted with the appropriate antisera followed by the detection system which contains anti-rabbit IgG/peroxidase conjugate and the substrate tetramethylbenzidine. Sixty-nine samples were tentatively considered to contain the above hemoglobin variants by isoelectrofocusing and the identity of 83% of them was confirmed by ELISA. Some of the non-reacting hemolysates were shown by amino acid sequence analysis to contain Hbs Korle-Bu, D-Ibadan, G-Copenhagen and the new variant Chandigarh. This ELISA offers specificity and simplicity for the confirmatory identification of hemoglobin variants. |
---|---|
AbstractList | We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their identification in hemolysates. Hbs in adult or cord blood hemolysates were coated to the wells of microtiter plates and reacted with the appropriate antisera followed by the detection system which contains anti-rabbit IgG/peroxidase conjugate and the substrate tetramethylbenzidine. Sixty-nine samples were tentatively considered to contain the above hemoglobin variants by isoelectrofocusing and the identity of 83% of them was confirmed by ELISA. Some of the non-reacting hemolysates were shown by amino acid sequence analysis to contain Hbs Korle-Bu, D-Ibadan, G-Copenhagen and the new variant Chandigarh. This ELISA offers specificity and simplicity for the confirmatory identification of hemoglobin variants. |
Author | Shyamala, M Kiefer, C R Garver, F A Moscoso, H |
Author_xml | – sequence: 1 givenname: H surname: Moscoso fullname: Moscoso, H organization: Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100 – sequence: 2 givenname: C R surname: Kiefer fullname: Kiefer, C R – sequence: 3 givenname: M surname: Shyamala fullname: Shyamala, M – sequence: 4 givenname: F A surname: Garver fullname: Garver, F A |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/2283294$$D View this record in MEDLINE/PubMed |
BookMark | eNotz81KAzEYheEsKrU_XoALITcwmuTLzOTbKaX-QMGNXZckk9hIk5TJVBivXotdHXgWB945maScHCG3nN0DZ_jAoAEmGmQMGXBEnJDZ2aozXpN5KV-McWyZnJKpEAoEyhl5XKefMToaYjylrEvRI_W5p8P-zzqXhuCD1UPIiWZP9y7mz0M2IdFv3QedhrIkV14firu57IJsn9cfq9dq8_7ytnraVFY2YqiM0MpL47lXIKHjtWuNq511CnittLVWeWWNQtsBINYSaxDGtgob1G0LYkHu_n-PJxNdtzv2Iep-3F1KxC9nw0uj |
CitedBy_id | crossref_primary_10_1002_jcla_1860070405 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.3109/03630269009031999 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine |
ExternalDocumentID | 2283294 |
Genre | Research Support, U.S. Gov't, P.H.S Journal Article |
GrantInformation_xml | – fundername: NHLBI NIH HHS grantid: HLB-41544 |
GroupedDBID | --- .GJ 00X 03L 0BK 0R~ 29I 36B 4.4 53G 5GY 5RE 5VS AALIY AALUX AAMIU AAORF AAPUL AAPXX AAQRR ABBKH ABDBF ABEIZ ABJNI ABLIJ ABLKL ABUPF ABWCV ABXYU ABZEW ACENM ACGEJ ACGFS ACIEZ ACKZS ADCVX ADFOM ADFZZ ADRBQ ADXPE AECIN AEIIZ AENEX AEOZL AFKVX AFLEI AFOSN AGDLA AGFJD AGRBW AGYJP AIJEM AIRBT AJVHN AJWEG AKBVH ALMA_UNASSIGNED_HOLDINGS ALQZU ALYBC AMDAE AWYRJ BABNJ BLEHA BOHLJ BRMBE CAG CCCUG CGR COF CS3 CUY CVF CYYVM CZDIS DKSSO DRXRE DU5 DWTOO EAP EBC EBD EBS ECM EIF EJD EMB EMK EMOBN EPL ESX F5P H13 HZ~ JENTW KRBQP KSSTO KWAYT KYCEM LJTGL M44 M4Z NPM NUSFT O9- QQXMO RNANH RVRKI SV3 TBQAZ TERGH TFDNU TFL TFW TUROJ TUS UEQFS V1S ZGI ZXP ~1N |
ID | FETCH-LOGICAL-c462t-b2a8f4bf1f8343d15e7be5ece83158accc8f8cb89cd3399549532bc78969a7732 |
ISSN | 0363-0269 |
IngestDate | Wed Oct 16 00:50:28 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 4 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c462t-b2a8f4bf1f8343d15e7be5ece83158accc8f8cb89cd3399549532bc78969a7732 |
PMID | 2283294 |
ParticipantIDs | pubmed_primary_2283294 |
PublicationCentury | 1900 |
PublicationDate | 1990-00-00 |
PublicationDateYYYYMMDD | 1990-01-01 |
PublicationDate_xml | – year: 1990 text: 1990-00-00 |
PublicationDecade | 1990 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Hemoglobin |
PublicationTitleAlternate | Hemoglobin |
PublicationYear | 1990 |
SSID | ssj0019704 |
Score | 1.3452184 |
Snippet | We have prepared monospecific antibodies to Hbs D-Los Angeles, J-Baltimore, O-Arab and J-Paris-I and developed an enzyme immunoassay (ELISA) for their... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 389 |
SubjectTerms | Adult Animals Antibody Specificity Enzyme-Linked Immunosorbent Assay Hemoglobin J - analysis Hemoglobin J - immunology Hemoglobins, Abnormal - analysis Hemoglobins, Abnormal - immunology Humans Immune Sera Immunoenzyme Techniques Infant, Newborn Isoelectric Focusing Rabbits |
Title | Enzyme immunoassay for the identification of hemoglobin variants |
URI | https://www.ncbi.nlm.nih.gov/pubmed/2283294 |
Volume | 14 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fT9swELYoaFNfEIxVA8bkB96qsDR2EucNhFqqIXgBJN6Q7dhapTVBa4XU_vXcxW6Sdj809mJFdmQ5_pzT3fm-O0JOFR_kxmgWhJqBgZLFJpBYMkzairkbq7CKJry5TcYP_Ntj_NgU46zYJXN1ppe_5ZX8D6rQB7giS_YNyNaTQgc8A77QAsLQ_hPGw2K5mJr-BDkeJWjBclFHDU5yHwbUUgmnJab_mBT9FzCQpc_htNJMx_VwjUE50-WsXGMwXE8wMW3lXW1CDe--L-RU_pBrztUr-dMHh468vzT3ZLuw5VtYcarwltcVU6nlJW-dC94SfswVA9oUyszlNMW5cKoQPUOY_KD9Luzr87RCCdPxRK7u8V8HN7Jk-5EO6aQCBd0tOm38ZVKWhry5sk4yd7mNy_r6y6K65J2fasPOqPSN-z2y6w0FeuFQ3ydbpvhA3t_4UIgDcu7Apy3wKYBPAXy6Dj4tLW3ApyvwP5KH0fD-chz4chiB5kk0D1QkheXKDqxgnOWD2KTKxEYbwQaxkFprYYVWItM5Q8IyRg5HSqciSzKZpizqke2iLMwnQtPYMizNakGZ5irMRAqiHQz7xOZ5zCQ_JD337U_PLufJk9-Uoz8NHJNuc4A-kx0Lv5g5AX1trr5UaLwCe6U6ww |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Enzyme+immunoassay+for+the+identification+of+hemoglobin+variants&rft.jtitle=Hemoglobin&rft.au=Moscoso%2C+H&rft.au=Kiefer%2C+C+R&rft.au=Shyamala%2C+M&rft.au=Garver%2C+F+A&rft.date=1990-01-01&rft.issn=0363-0269&rft.volume=14&rft.issue=4&rft.spage=389&rft_id=info:doi/10.3109%2F03630269009031999&rft_id=info%3Apmid%2F2283294&rft_id=info%3Apmid%2F2283294&rft.externalDocID=2283294 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0363-0269&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0363-0269&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0363-0269&client=summon |