Optimization of purification and refolding of the human chemokine receptor CXCR1 improves the stability of proteoliposomes for structure determination - Park, Sang Ho; Casagrande, Fabio; Chu, Mignon; Maier, Klaus; Kiefer, Hans; Opella, Stanley J. (Biochimica et biophysica acta, 2012)
Keywords: CXCR1; Escherichia coli - chemistry; Escherichia coli - genetics; Escherichia coli - metabolism; GPCR; Humans; Hydrogen-Ion Concentration; Lipid Bilayers - chemistry; Liposomes - chemistry; Phospholipid bilayer; Phospholipids - chemistry; Protein Folding; Protein Stability; Protein Structure, Tertiary; Proteoliposome; Receptors, Interleukin-8A - biosynthesis; Receptors, Interleukin-8A - chemistry; Receptors, Interleukin-8A - genetics; Receptors, Interleukin-8A - isolation & purification; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Solid-state NMR; Solid-state NMR; SDS; OS; Phospholipid bilayer; GST; Proteoliposome; CXCR1; DPC; HEPES; MβCD; DHPC; GPCR; HPC; DMPC; TCEP; MAS