Deletion of the Pemt gene increases progenitor cell mitosis, DNA and protein methylation and decreases calretinin expression in embryonic day 17 mouse hippocampus

• Published in: Brain research. Developmental brain research Vol. 149; no. 2; pp. 121 - 129
• Main Authors: Zhu, Xiaonan, Mar, Mei-Heng, Song, Jiannan, Zeisel, Steven H
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.04.2004
• Subjects: Acyltransferases - genetics; Animals; Blotting, Western - methods; Brain - embryology; Brain - metabolism; Calbindin 2; Calretinin; Choline; Choline - metabolism; Chromatography, High Pressure Liquid - methods; Diet; DNA - metabolism; Embryo, Mammalian; Female; Gene Deletion; Gene Expression Regulation, Developmental; Hippocampus; Hippocampus - cytology; Hippocampus - embryology; Hippocampus - metabolism; Histones - metabolism; Liver - embryology; Liver - metabolism; Male; Memory; Methylation; Methyltransferases - genetics; Methyltransferases - physiology; Mice; Mice, Knockout; Mitosis; Mitosis - physiology; Neurons - physiology; PEMT; Phosphatidylethanolamine N-Methyltransferase; Pregnancy; Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Messenger - biosynthesis; S-Adenosylmethionine - metabolism; S100 Calcium Binding Protein G - metabolism; Stem Cells - physiology; Pregnancy; Calretinin; Mitosis; Choline; Memory; PEMT; Methylation; Hippocampus

Choline is a required nutrient and is derived from the diet as well as from de novo synthesis catalyzed by phosphatidylethanolamine N-methyltransferase (PEMT). We previously reported that choline availability during pregnancy alters mitosis and neuronal protein expression during fetal brain developm...