Effects of Ginkgo biloba Extract on Spinal Cord Ischemia–Reperfusion Injury in Rats

• Published in: Annals of vascular surgery Vol. 28; no. 5; pp. 1296 - 1305
• Main Authors: Badem, Serdar, Ugurlucan, Murat, El, Helin, Sahin, Mazlum, Uysal, Mujdat, Sayin, Omer Ali, Gurel, Bora, Basaran, Murat, Bayindir, Cicek, Alpagut, Ufuk, Dayioglu, Enver
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.07.2014
• Subjects: Animals; Disease Models, Animal; Ginkgo biloba; Malondialdehyde - metabolism; Oxidative Stress - drug effects; Phytotherapy - methods; Plant Extracts - therapeutic use; Rats; Rats, Sprague-Dawley; Reperfusion Injury - drug therapy; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Spinal Cord Ischemia - drug therapy; Spinal Cord Ischemia - metabolism; Spinal Cord Ischemia - pathology; Surgery; Treatment Outcome
• ISSN: 0890-5096; 1615-5947; 1615-5947
• DOI: 10.1016/j.avsg.2014.02.020

We aimed to assess the biochemical and histopathologic effects of Ginkgo biloba extract (EGb) in an ischemia–reperfusion (IR) model of spinal cord ischemia induced by cross-clamping of the infrarenal abdominal aorta. A total of 24 Sprague–Dawley rats were divided into 3 groups as group 1: control (sham laparotomy), group 2: IR, and group 3: IR + EGb treatment (IR + T) group. All subjects were euthanized 2 days postsurgery and their spinal cords were removed. Tissue malondialdehyde, superoxide dismutase, glutathione (GSH), and glutathione peroxidase levels were measured, and the spinal cord tissue samples were examined histopathologically. No significant difference was detected in ischemia markers between control, IR, and IR + T groups, with the exception of GSH, which was significantly lower in the IR group. GSH levels in group 1 and group 3 were similar. The group 2 displayed significant ischemic damage to the medulla spinalis. This damage was less pronounced in group 3 compared with group 2 only, but in extent and intensity comparable with the controls. Although we were not able to demonstrate a uniform effect of EGb on biochemical markers of IR injury, the histopathologic data appear to show a protective effect conferred on the spinal cord tissue by EGb.