Toward an understanding of the biogenesis of the starch granule. Evidence that Chlamydomonas soluble starch synthase II controls the synthesis of intermediate size glucans of amylopectin

• Published in: The Journal of biological chemistry Vol. 268; no. 22; pp. 16223 - 16230
• Main Authors: Fontaine, T, D'Hulst, C, Maddelein, M.L, Routier, F, Pepin, T.M, Decq, A, Wieruszeski, J.M, Delrue, B, Koornhuyse, N. van den, Bossu, J.P
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 05.08.1993
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ALMIDON; AMIDON; AMILOPECTINA; AMILOSA; Amylopectin; Amylopectin - biosynthesis; AMYLOPECTINE; AMYLOSE; Amylose - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carbohydrates; Chlamydomonas; Chlamydomonas - genetics; Chlamydomonas - metabolism; CHLOROPHYCEAE; Chromatography; Fundamental and applied biological sciences. Psychology; GLICOSILTRANSFERASAS; GLUCANE; GLUCANO; GLYCOSYLTRANSFERASE; Holosides; Life Sciences; Magnetic Resonance Spectroscopy; METABOLISME DES GLUCIDES; METABOLISMO DE CARBOHIDRATOS; MUTANT; MUTANTES; Mutation; Other biological molecules; Solubility; Starch Synthase; Starch Synthase - metabolism; Enzyme; Starch; Algae; Chlamydomonas reinhardtii; Chlorophycophyta; Carbohydrate; Biosynthesis; Synthase; Thallophyta

Low starch mutants of Chlamydomonas reinhardtii were isolated after x-ray mutagenesis of wild-type strain 137C. The mutants accumulated 20-40% of the normal amount and displayed a 2-fold decrease of the total glycogen-primed soluble starch synthase activity. Three different mutant alleles of the st-...