Cytotoxicity of WT1-reactive T cells against Wilms tumor: An implication for antigen-specific adoptive immunotherapy

• Published in: Bioimpacts Vol. 13; no. 5; pp. 415 - 424
• Main Authors: Monzavi, Seyed Mostafa, Hamidieh, Amir Ali, Vasei, Mohammad, Ai, Jafar, Ahmadbeigi, Naser, Arshadi, Hamid, Muhammadnejad, Samad, Kajbafzadeh, Abdol-Mohammad
• Format: Journal Article
• Language: English
• Published: Tabriz Tabriz University of Medical Sciences 2023; Tabriz University of Medical Sciences (TUOMS Publishing Group)
• Subjects: Acetic acid; Adenomatous polyposis coli; Adoptive immunotherapy; Antibodies; Antigen-presenting cells; Antigens; Blood & organ donations; Bovine serum albumin; Cancer; CD3 antigen; CD4 antigen; CD8 antigen; Chemotherapy; Cloning; Collagenase; Cytokines; Cytotoxicity; cytotoxicity tests; Flow cytometry; Genetic engineering; immunomagnetic separation; Immunotherapy; Isolation media; Lymphocytes; Lymphocytes T; Original; Pediatrics; Peptides; Peripheral blood mononuclear cells; T cell receptors; t-lymphocytes; Tumors; wilms tumor; wt1; Iran; United States > US; Germany

Introduction: T cells that recognize WT1 peptides have been shown to efficiently eliminate WT1-expressing tumor cells. This study was designed to investigate the feasibility of isolating WT1-reactive T cells from peripheral blood mononuclear cells (PBMCs) from healthy donors and patients with Wilms...