Reference Grade Characterization of Polymorphisms in Full-Length HLA Class I and II Genes With Short-Read Sequencing on the ION PGM System and Long-Reads Generated by Single Molecule, Real-Time Sequencing on the PacBio Platform

• Published in: Frontiers in immunology Vol. 9; p. 2294
• Main Authors: Suzuki, Shingo, Ranade, Swati, Osaki, Ken, Ito, Sayaka, Shigenari, Atsuko, Ohnuki, Yuko, Oka, Akira, Masuya, Anri, Harting, John, Baybayan, Primo, Kitazume, Miwako, Sunaga, Junichi, Morishima, Satoko, Morishima, Yasuo, Inoko, Hidetoshi, Kulski, Jerzy K., Shiina, Takashi
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 04.10.2018
• Subjects: Adult; Aged; Aged, 80 and over; Alleles; Arthritis, Rheumatoid - genetics; Computational Biology - methods; Female; Gene Frequency; Genes, MHC Class I; Genes, MHC Class II; Genetic Association Studies; Genetic Predisposition to Disease; Genomics - methods; Genotype; genotyping; Genotyping Techniques; High-Throughput Nucleotide Sequencing; HLA; human leukocyte antigen; Humans; Immunology; Male; Middle Aged; next-generation sequencing; NGS; Phylogeny; Polymorphism, Genetic; Sequence Analysis, DNA; SMRT sequencing; Software; Ion PGM; SMRT sequencing; human leukocyte antigen; next-generation sequencing; genotyping; NGS; HLA; PacBio RS II

Although NGS technologies fuel advances in high-throughput HLA genotyping methods for identification and classification of HLA genes to assist with precision medicine efforts in disease and transplantation, the efficiency of these methods are impeded by the absence of adequately-characterized high-f...