Validating maps from single particle electron cryomicroscopy

• Published in: Current opinion in structural biology Vol. 34; pp. 135 - 144
• Main Authors: Rosenthal, Peter B, Rubinstein, John L
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2015
• Subjects: Cryoelectron Microscopy - methods; Cryoelectron Microscopy - standards; Crystallography, X-Ray; Models, Molecular; Reproducibility of Results

•Single particle cryo-EM maps should be validated by specific tests.•Tilt-pair analysis shows that a map is consistent with image data at low resolution.•Map resolution should be consistent with map features and tests for over-fitting.•Maps at low or high resolution may be validated by fitting an at...