In vitro assessment of cord blood–derived proinsulin-specific regulatory T cells for cellular therapy in type 1 diabetes
Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore,...
Saved in:
| Published in | Cytotherapy (Oxford, England) Vol. 20; no. 11; pp. 1355 - 1370 |
|---|---|
| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Inc
01.11.2018
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 1465-3249 1477-2566 1477-2566 |
| DOI | 10.1016/j.jcyt.2018.09.004 |
Cover
Loading…
| Abstract | Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers.
We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays.
Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern.
Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D. |
|---|---|
| AbstractList | Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers.
We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays.
Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern.
Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D. Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers.BACKGROUNDAntigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers.We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays.METHODSWe analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays.Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern.RESULTSFollowing stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern.Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D.CONCLUSIONOur study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D. Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers. We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127 and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays. Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D (P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg (P = 0.01) and iTreg (P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly (P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern. Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D. Abstract Background Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can serve as an efficient and safe source for Tregs for antigen-specific immunomodulation in T1D, a strategy that is yet to be explored. Therefore, we assessed the potential of CB in generation of proinsulin (PI)-specific Tregs by using HLA class II tetramers. Methods We analyzed the frequency of PI-specific natural Tregs (nTregs) and induced Tregs (iTregs) derived from the CB as well as peripheral blood (PB) of patients with T1D and healthy control subjects. For this, CD4+CD25+CD127low and CD4+CD25-T cells were cultured in the presence of PI-derived peptides, transforming growth factor (TGF)-β and rapamycin. PI-specific Tregs were then selected using allele-specific HLA II tetramers loaded with PI-derived peptides, followed by suppression assays. Results Following stimulation, we observed that CB harbors a significantly higher frequency of PI-specific Tregs than PB of subjects with T1D ( P = 0.0003). Further, the proportion of PI-specific Tregs was significantly higher in both the nTreg ( P = 0.01) and iTreg ( P = 0.0003) compartments of CB as compared with PB of subjects with T1D. In co-culture experiments, the PI-specific Tregs suppressed the proliferation of effector T cells significantly ( P = 0.0006). The expanded nTregs were able to retain hypomethylation status at their Tregs-specific demethylated region (TSDR), whereas iTregs were unable to acquire the characteristic demethylation pattern. Conclusion Our study demonstrates that CB can serve as an excellent source for generation of functional antigen-specific Tregs for immunotherapeutic approaches in subjects with T1D. |
| Author | SACHDEVA, NARESH DAYAL, DEVI BHANSALI, ANIL DHALIWAL, LAKHBIR Paul, MAHINDER |
| Author_xml | – sequence: 1 givenname: MAHINDER surname: Paul fullname: Paul, MAHINDER organization: Departments of Endocrinology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India – sequence: 2 givenname: DEVI orcidid: 0000-0002-3279-6002 surname: DAYAL fullname: DAYAL, DEVI organization: Departments of Pediatrics, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India – sequence: 3 givenname: ANIL surname: BHANSALI fullname: BHANSALI, ANIL organization: Departments of Endocrinology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India – sequence: 4 givenname: LAKHBIR surname: DHALIWAL fullname: DHALIWAL, LAKHBIR organization: Departments of Obstetrics and Gynecology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India – sequence: 5 givenname: NARESH orcidid: 0000-0001-6289-4749 surname: SACHDEVA fullname: SACHDEVA, NARESH email: naresh_pgi@hotmail.com organization: Departments of Endocrinology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/30340983$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFUstu1TAUjFARfcAPsEBesknwI7EThJBQxaNSJRaUteXYJ-CQawfbuVJY8Q_9w34JDrfdVKIsLB9ZM3OOZ85pceS8g6J4TnBFMOGvxmrUa6ooJm2Fuwrj-lFxQmohStpwfrTVvCkZrbvj4jTGEWOK27Z5UhwzzGrcteyk-HXh0N6m4JGKEWLcgUvID0j7YFA_eW9ufl8bCHYPBs3BWxeXyboyzqDtYDUK8G2ZVPJhRVdIwzRFNPjwt8rvAaXvENS8IutQWmdABBmrekgQnxaPBzVFeHZ7nxVfP7y_Ov9UXn7-eHH-7rLUNaepbITSlAshWqJg4MZgwbFuespJI4Tq8iGU94pBj-thoAPrRG3aFlhLOOMDOyteHnTz-D8XiEnubNzmUw78EiUllAnCMesy9MUtdOl3YOQc7E6FVd75lQHtAaCDjzHAILVNKlnvUlB2kgTLLRo5yi0auUUjcSdzNJlK71Hv1B8kvTmQIBu0txBk1BacBmMD6CSNtw_T396j6xye1Wr6ASvE0S_BZeslkZFKLL9sC7PtC2kZ5oRt_339b4H_df8DcYjSVQ |
| CitedBy_id | crossref_primary_10_4103_jod_jod_46_20 crossref_primary_10_4103_jod_jod_77_24 crossref_primary_10_1007_s13410_021_00919_7 crossref_primary_10_1134_S1990519X22010047 crossref_primary_10_3389_fimmu_2024_1462384 crossref_primary_10_1016_j_clim_2023_109716 crossref_primary_10_1007_s40200_022_01120_1 crossref_primary_10_3389_fimmu_2020_00611 crossref_primary_10_1186_s13098_024_01277_0 |
| Cites_doi | 10.1182/blood-2004-06-2467 10.4049/jimmunol.1600676 10.1016/S1074-7613(00)80048-5 10.1182/blood-2007-06-094482 10.1182/blood-2006-10-052506 10.1186/1471-2105-11-230 10.1182/blood-2004-10-3932 10.1101/cshperspect.a007781 10.2337/diabetes.54.1.92 10.1111/pedi.12536 10.4049/jimmunol.1401145 10.1002/eji.200636435 10.1182/blood.V93.7.2302 10.1186/s13072-017-0129-1 10.1016/j.jaut.2005.09.018 10.2337/dc12-0038 10.1111/pedi.12029 10.1111/j.1399-3038.2008.00843.x 10.1016/j.exphem.2005.09.002 10.1002/dmrr.1244 10.4049/jimmunol.1004112 10.1093/intimm/dxm014 10.4049/jimmunol.181.12.8209 10.1097/01.mjt.0000178767.67857.63 10.2337/db11-1520 10.1016/j.clim.2014.03.016 10.1016/j.omtm.2016.12.003 10.1371/journal.pone.0029355 10.1016/j.jaut.2016.03.011 10.1371/journal.pone.0005994 10.2337/db16-1025 10.1084/jem.192.2.303 10.1002/eji.201141651 10.2337/dc09-0967 10.1182/blood-2010-07-293795 10.3324/haematol.2012.074088 10.1007/s00005-012-0177-y 10.1007/s00277-010-1121-z 10.4049/jimmunol.0901065 10.1038/ni1572 10.1007/s10753-014-9878-1 10.1111/cei.12116 10.1002/eji.200838819 10.1172/JCI19441 10.1038/ni.1818 10.1093/jmcb/mjr039 10.1016/j.clim.2015.01.005 10.2337/db14-0332 |
| ContentType | Journal Article |
| Copyright | 2018 International Society for Cellular Therapy International Society for Cellular Therapy Copyright © 2018 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
| Copyright_xml | – notice: 2018 International Society for Cellular Therapy – notice: International Society for Cellular Therapy – notice: Copyright © 2018 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
| DBID | AAYXX CITATION NPM 7X8 |
| DOI | 10.1016/j.jcyt.2018.09.004 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic PubMed |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Pharmacy, Therapeutics, & Pharmacology |
| EISSN | 1477-2566 |
| EndPage | 1370 |
| ExternalDocumentID | 30340983 10_1016_j_jcyt_2018_09_004 S1465324918306133 1_s2_0_S1465324918306133 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GroupedDBID | --- --M .1- .FO .~1 1P~ 1~. 29F 36B 4.4 457 4G. 53G 5GY 5VS 7-5 8P~ AAAJQ AAEDT AAEDW AAIKJ AAKOC AALRI AAOAW AATTM AAXKI AAXUO AAYWO ABDBF ABGSF ABJNI ABMAC ABMZM ABUDA ABXDB ACDAQ ACGEJ ACGFS ACIEU ACRLP ACUHS ACVFH ADBBV ADCNI ADCVX ADEZE ADXPE AEBSH AEHWI AEIPS AEKER AENEX AEUPX AEVXI AFJKZ AFKVX AFPUW AFRHN AFTJW AFXIZ AGCQF AGEKW AGHFR AGRDE AGUBO AGYEJ AIEXJ AIGII AIIUN AIKHN AITUG AJRQY AJUYK AJWEG AKBMS AKRWK AKYEP ALMA_UNASSIGNED_HOLDINGS AMRAJ ANKPU ANZVX APXCP ASPBG AVWKF AWYRJ AXJTR AZFZN BKOJK BLXMC BNPGV CAG CJTIS COF CS3 DU5 EAP EBC EBS EFJIC EFKBS EJD EMB EMK EMOBN ESX F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN GBLVA H13 HVGLF HZ~ KOM LUGTX M44 MO0 O-L O9. OAUVE OK~ P-8 P-9 P2P PC. Q38 R2- ROL SDF SPCBC SSH SSI SSU SSZ SV3 T5K TDBHL TFW Z5R ~G- AACTN AFCTW AFKWA AJOXV AMFUW AAIAV ABLVK ABYKQ AJBFU DOVZS EFLBG LCYCR AAYXX AGRNS CITATION NPM 7X8 |
| ID | FETCH-LOGICAL-c462t-57ac2677781aef6dd0760c5b261577a977a126ba3eb04ff2f3974d88e381636f3 |
| IEDL.DBID | .~1 |
| ISSN | 1465-3249 1477-2566 |
| IngestDate | Fri Jul 11 02:44:12 EDT 2025 Wed Feb 19 02:32:29 EST 2025 Thu Apr 24 23:09:46 EDT 2025 Tue Jul 01 03:06:22 EDT 2025 Fri Feb 23 02:16:04 EST 2024 Tue Feb 25 19:54:30 EST 2025 Tue Aug 26 17:16:31 EDT 2025 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Keywords | regulatory T cells proinsulin cord blood type 1 diabetes HLA tetramers |
| Language | English |
| License | Copyright © 2018 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c462t-57ac2677781aef6dd0760c5b261577a977a126ba3eb04ff2f3974d88e381636f3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0002-3279-6002 0000-0001-6289-4749 |
| PMID | 30340983 |
| PQID | 2123716039 |
| PQPubID | 23479 |
| PageCount | 16 |
| ParticipantIDs | proquest_miscellaneous_2123716039 pubmed_primary_30340983 crossref_citationtrail_10_1016_j_jcyt_2018_09_004 crossref_primary_10_1016_j_jcyt_2018_09_004 elsevier_sciencedirect_doi_10_1016_j_jcyt_2018_09_004 elsevier_clinicalkeyesjournals_1_s2_0_S1465324918306133 elsevier_clinicalkey_doi_10_1016_j_jcyt_2018_09_004 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2018-11-01 |
| PublicationDateYYYYMMDD | 2018-11-01 |
| PublicationDate_xml | – month: 11 year: 2018 text: 2018-11-01 day: 01 |
| PublicationDecade | 2010 |
| PublicationPlace | England |
| PublicationPlace_xml | – name: England |
| PublicationTitle | Cytotherapy (Oxford, England) |
| PublicationTitleAlternate | Cytotherapy |
| PublicationYear | 2018 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Ryba-Stanislawowska, Rybarczyk-Kapturska, Mysliwiec, Mysliwska (bib0010) 2014; 37 Haseda, Imagawa, Murase-Mishiba, Terasaki, Hanafusa (bib0012) 2013; 173 Savage, Boniface, Davis (bib0049) 1999; 10 Zeiser, Leveson-Gower, Zambricki, Kambham, Beilhack, Loh (bib0023) 2008; 111 Battaglia, Stabilini, Roncarolo (bib0022) 2005; 105 Paul, Badal, Jacob, Dayal, Kumar, Bhansali (bib0033) 2018; 19 Scotta, Esposito, Fazekasova, Fanelli, Edozie, Ali (bib0037) 2013; 98 Tang, Bluestone (bib0008) 2008; 9 Marek-Trzonkowska, Mysliwiec, Dobyszuk, Grabowska, Derkowska, Juscinska (bib0014) 2014; 153 Lan, Fan, Quesniaux, Ryffel, Liu, Zheng (bib0007) 2012; 4 Lee, Lin, Cheng, Kuo (bib0018) 2009; 20 Lindley, Dayan, Bishop, Roep, Peakman, Tree (bib0011) 2005; 54 Huter, Stummvoll, DiPaolo, Glass, Shevach (bib0047) 2008; 181 Godfrey, Spoden, Ge, Baker, Liu, Levine (bib0017) 2005; 105 Michels, Landry, McDaniel, Yu, Campbell-Thompson, Kwok (bib0003) 2017; 66 Mayer, Bannert, Gruber, Klunker, Spittler, Akdis (bib0044) 2012; 7 Yoon, Jun (bib0002) 2005; 12 Allan, Crome, Crellin, Passerini, Steiner, Bacchetta (bib0042) 2007; 19 Wang, Huizinga, Toes (bib0045) 2009; 183 Nguyen, Sullivan, Ebel, Teague, DiPaolo (bib0046) 2011; 187 Marek-Trzonkowska, Mysliwiec, Dobyszuk, Grabowska, Techmanska, Juscinska (bib0015) 2012; 35 Seay, Putnam, Cserny, Posgai, Rosenau, Wingard (bib0019) 2017; 4 James, LaFond, Durinovic-Bello, Kwok (bib0026) 2009; 6 Rohde, Zhang, Reinhardt, Jeltsch (bib0028) 2010; 11 Skowera, Ladell, McLaren, Dolton, Matthews, Gostick (bib0035) 2015; 64 Sachdeva, Paul, Badal, Kumar, Jacob, Dayal (bib0034) 2015; 157 Walker, Kasprowicz, Gersuk, Benard, Van Landeghen, Buckner (bib0038) 2003; 112 Szypowska, Stelmaszczyk-Emmel, Demkow, Luczynski (bib0009) 2012; 60 Danova, Grohova, Strnadova, Funda, Sumnik, Lebl (bib0013) 2017; 198 McMurchy, Levings (bib0027) 2012; 42 James, Mallone, Schloot, Gagnerault, Thorpe, Fitzgerald-Miller (bib0025) 2011; 27 Gitelman, Bluestone (bib0006) 2016; 71 Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi (bib0004) 2000; 192 Hamdi, Godot, Maillot, Prejean, Cohen, Krzysiek (bib0039) 2007; 110 Okada, Kanamori, Someya, Nakatsukasa, Yoshimura (bib0048) 2017; 10 Long, Walker, Rieck, James, Kwok, Sanda (bib0030) 2009; 39 Brunstein, Miller, Cao, McKenna, Hippen, Curtsinger (bib0032) 2011; 117 Wang, Ioan-Facsinay, van der Voort, Huizinga, Toes (bib0043) 2007; 37 Roep, Peakman (bib0001) 2012; 2 Rossetti, Spreafico, Saidin, Chua, Moshref, Leong (bib0031) 2015; 194 Haller, Wasserfall, McGrail, Cintron, Brusko, Wingard (bib0024) 2009; 32 Strauss, Czystowska, Szajnik, Mandapathil, Whiteside (bib0021) 2009; 4 Kronenberg, Knight, Estorninho, Ellis, Kester, de Ru (bib0036) 2012; 61 Sorg, Kogler, Wernet (bib0041) 1999; 93 Mayer, Bannert, Gruber, Klunker, Spittler, Akdis (bib0029) 2012; 7 Chang, Satwani, Oberfield, Vlad, Simpson, Cairo (bib0040) 2005; 33 Marek-Trzonkowska, Mysliwec, Siebert, Trzonkowski (bib0016) 2013; 14 Wing, Sakaguchi (bib0005) 2010; 11 Asanuma, Tanaka, Sugita, Kosugi, Shiratori, Wakasa (bib0020) 2011; 90 Oling, Marttila, Ilonen, Kwok, Nepom, Knip (bib0050) 2005; 25 Gitelman (10.1016/j.jcyt.2018.09.004_bib0006) 2016; 71 Chang (10.1016/j.jcyt.2018.09.004_bib0040) 2005; 33 Strauss (10.1016/j.jcyt.2018.09.004_bib0021) 2009; 4 Marek-Trzonkowska (10.1016/j.jcyt.2018.09.004_bib0015) 2012; 35 Kronenberg (10.1016/j.jcyt.2018.09.004_bib0036) 2012; 61 Zeiser (10.1016/j.jcyt.2018.09.004_bib0023) 2008; 111 Sorg (10.1016/j.jcyt.2018.09.004_bib0041) 1999; 93 James (10.1016/j.jcyt.2018.09.004_bib0025) 2011; 27 Godfrey (10.1016/j.jcyt.2018.09.004_bib0017) 2005; 105 Mayer (10.1016/j.jcyt.2018.09.004_bib0044) 2012; 7 Brunstein (10.1016/j.jcyt.2018.09.004_bib0032) 2011; 117 Savage (10.1016/j.jcyt.2018.09.004_bib0049) 1999; 10 Seay (10.1016/j.jcyt.2018.09.004_bib0019) 2017; 4 Sachdeva (10.1016/j.jcyt.2018.09.004_bib0034) 2015; 157 Skowera (10.1016/j.jcyt.2018.09.004_bib0035) 2015; 64 Ryba-Stanislawowska (10.1016/j.jcyt.2018.09.004_bib0010) 2014; 37 Mayer (10.1016/j.jcyt.2018.09.004_bib0029) 2012; 7 Okada (10.1016/j.jcyt.2018.09.004_bib0048) 2017; 10 Michels (10.1016/j.jcyt.2018.09.004_bib0003) 2017; 66 Wang (10.1016/j.jcyt.2018.09.004_bib0043) 2007; 37 Takahashi (10.1016/j.jcyt.2018.09.004_bib0004) 2000; 192 Marek-Trzonkowska (10.1016/j.jcyt.2018.09.004_bib0014) 2014; 153 Scotta (10.1016/j.jcyt.2018.09.004_bib0037) 2013; 98 Roep (10.1016/j.jcyt.2018.09.004_bib0001) 2012; 2 Marek-Trzonkowska (10.1016/j.jcyt.2018.09.004_bib0016) 2013; 14 Wing (10.1016/j.jcyt.2018.09.004_bib0005) 2010; 11 Battaglia (10.1016/j.jcyt.2018.09.004_bib0022) 2005; 105 James (10.1016/j.jcyt.2018.09.004_bib0026) 2009; 6 Lee (10.1016/j.jcyt.2018.09.004_bib0018) 2009; 20 Long (10.1016/j.jcyt.2018.09.004_bib0030) 2009; 39 Haller (10.1016/j.jcyt.2018.09.004_bib0024) 2009; 32 Lindley (10.1016/j.jcyt.2018.09.004_bib0011) 2005; 54 Rossetti (10.1016/j.jcyt.2018.09.004_bib0031) 2015; 194 Haseda (10.1016/j.jcyt.2018.09.004_bib0012) 2013; 173 Wang (10.1016/j.jcyt.2018.09.004_bib0045) 2009; 183 Paul (10.1016/j.jcyt.2018.09.004_bib0033) 2018; 19 Yoon (10.1016/j.jcyt.2018.09.004_bib0002) 2005; 12 Rohde (10.1016/j.jcyt.2018.09.004_bib0028) 2010; 11 Oling (10.1016/j.jcyt.2018.09.004_bib0050) 2005; 25 Tang (10.1016/j.jcyt.2018.09.004_bib0008) 2008; 9 Walker (10.1016/j.jcyt.2018.09.004_bib0038) 2003; 112 Lan (10.1016/j.jcyt.2018.09.004_bib0007) 2012; 4 Asanuma (10.1016/j.jcyt.2018.09.004_bib0020) 2011; 90 Danova (10.1016/j.jcyt.2018.09.004_bib0013) 2017; 198 Allan (10.1016/j.jcyt.2018.09.004_bib0042) 2007; 19 McMurchy (10.1016/j.jcyt.2018.09.004_bib0027) 2012; 42 Nguyen (10.1016/j.jcyt.2018.09.004_bib0046) 2011; 187 Huter (10.1016/j.jcyt.2018.09.004_bib0047) 2008; 181 Hamdi (10.1016/j.jcyt.2018.09.004_bib0039) 2007; 110 Szypowska (10.1016/j.jcyt.2018.09.004_bib0009) 2012; 60 |
| References_xml | – volume: 14 start-page: 322 year: 2013 end-page: 332 ident: bib0016 article-title: Clinical application of regulatory T cells in type 1 diabetes publication-title: Pediatr Diabetes – volume: 4 start-page: 22 year: 2012 end-page: 28 ident: bib0007 article-title: Induced Foxp3+ regulatory T cells: a potential new weapon to treat autoimmune and inflammatory diseases? publication-title: Journal of molecular cell biology – volume: 173 start-page: 207 year: 2013 end-page: 216 ident: bib0012 article-title: CD4(+) CD45RA(-) FoxP3high activated regulatory T cells are functionally impaired and related to residual insulin-secreting capacity in patients with type 1 diabetes publication-title: Clin Exp Immunol – volume: 12 start-page: 580 year: 2005 end-page: 591 ident: bib0002 article-title: Autoimmune destruction of pancreatic β cells publication-title: American journal of therapeutics – volume: 98 start-page: 1291 year: 2013 end-page: 1299 ident: bib0037 article-title: Differential effects of rapamycin and retinoic acid on expansion, stability and suppressive qualities of human CD4(+)CD25(+)FOXP3(+) T regulatory cell subpopulations publication-title: Haematologica – volume: 54 start-page: 92 year: 2005 end-page: 99 ident: bib0011 article-title: Defective suppressor function in CD4(+)CD25(+) T-cells from patients with type 1 diabetes publication-title: Diabetes – volume: 90 start-page: 617 year: 2011 end-page: 624 ident: bib0020 article-title: Expansion of CD4+ CD25+ regulatory T cells from cord blood CD4+ cells using the common γ-chain cytokines (IL-2 and IL-15) and rapamycin publication-title: Annals of hematology – volume: 11 start-page: 230 year: 2010 ident: bib0028 article-title: BISMA–fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences publication-title: BMC Bioinformatics – volume: 105 start-page: 4743 year: 2005 end-page: 4748 ident: bib0022 article-title: Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cells publication-title: Blood – volume: 110 start-page: 211 year: 2007 end-page: 219 ident: bib0039 article-title: Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper publication-title: Blood – volume: 112 start-page: 1437 year: 2003 end-page: 1443 ident: bib0038 article-title: Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4+CD25- T cells publication-title: J Clin Invest – volume: 11 start-page: 7 year: 2010 end-page: 13 ident: bib0005 article-title: Regulatory T cells exert checks and balances on self tolerance and autoimmunity publication-title: Nature immunology – volume: 66 start-page: 722 year: 2017 end-page: 734 ident: bib0003 article-title: Islet-Derived CD4 T Cells Targeting Proinsulin in Human Autoimmune Diabetes publication-title: Diabetes – volume: 37 start-page: 129 year: 2007 end-page: 138 ident: bib0043 article-title: Transient expression of FOXP3 in human activated nonregulatory CD4+ T cells publication-title: Eur J Immunol – volume: 198 start-page: 729 year: 2017 end-page: 740 ident: bib0013 article-title: Tolerogenic Dendritic Cells from Poorly Compensated Type 1 Diabetes Patients Have Decreased Ability To Induce Stable Antigen-Specific T Cell Hyporesponsiveness and Generation of Suppressive Regulatory T Cells publication-title: J Immunol – volume: 33 start-page: 1508 year: 2005 end-page: 1520 ident: bib0040 article-title: Increased induction of allogeneic-specific cord blood CD4+CD25+ regulatory T (Treg) cells: a comparative study of naive and antigenic-specific cord blood Treg cells publication-title: Exp Hematol – volume: 9 start-page: 239 year: 2008 end-page: 244 ident: bib0008 article-title: The Foxp3+ regulatory T cell: a jack of all trades, master of regulation publication-title: Nature immunology – volume: 105 start-page: 750 year: 2005 end-page: 758 ident: bib0017 article-title: Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function publication-title: Blood – volume: 6 start-page: 1167 year: 2009 ident: bib0026 article-title: Visualizing antigen specific CD4+ T cells using MHC class II tetramers publication-title: J Vis Exp – volume: 19 start-page: 345 year: 2007 end-page: 354 ident: bib0042 article-title: Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production publication-title: International immunology – volume: 4 start-page: e5994 year: 2009 ident: bib0021 article-title: Differential responses of human regulatory T cells (Treg) and effector T cells to rapamycin publication-title: PLoS One – volume: 4 start-page: 178 year: 2017 end-page: 191 ident: bib0019 article-title: Expansion of Human Tregs from Cryopreserved Umbilical Cord Blood for GMP-Compliant Autologous Adoptive Cell Transfer Therapy publication-title: Mol Ther Methods Clin Dev – volume: 42 start-page: 27 year: 2012 end-page: 34 ident: bib0027 article-title: Suppression assays with human T regulatory cells: a technical guide publication-title: Eur J Immunol – volume: 192 start-page: 303 year: 2000 end-page: 310 ident: bib0004 article-title: Immunologic self-tolerance maintained by CD25+ CD4+ regulatory T cells constitutively expressing cytotoxic T lymphocyte–associated antigen 4 publication-title: The Journal of experimental medicine – volume: 93 start-page: 2302 year: 1999 end-page: 2307 ident: bib0041 article-title: Identification of cord blood dendritic cells as an immature CD11c- population publication-title: Blood – volume: 111 start-page: 453 year: 2008 end-page: 462 ident: bib0023 article-title: Differential impact of mammalian target of rapamycin inhibition on CD4+CD25+Foxp3+ regulatory T cells compared with conventional CD4+ T cells publication-title: Blood – volume: 60 start-page: 307 year: 2012 end-page: 313 ident: bib0009 article-title: Low frequency of regulatory T cells in the peripheral blood of children with type 1 diabetes diagnosed under the age of five publication-title: Arch Immunol Ther Exp (Warsz) – volume: 10 start-page: 485 year: 1999 end-page: 492 ident: bib0049 article-title: A kinetic basis for T cell receptor repertoire selection during an immune response publication-title: Immunity – volume: 32 start-page: 2041 year: 2009 end-page: 2046 ident: bib0024 article-title: Autologous umbilical cord blood transfusion in very young children with type 1 diabetes publication-title: Diabetes Care – volume: 61 start-page: 1752 year: 2012 end-page: 1759 ident: bib0036 article-title: Circulating preproinsulin signal peptide-specific CD8 T cells restricted by the susceptibility molecule HLA-A24 are expanded at onset of type 1 diabetes and kill beta-cells publication-title: Diabetes – volume: 194 start-page: 113 year: 2015 end-page: 124 ident: bib0031 article-title: -expanded but not publication-title: J Immunol – volume: 19 start-page: 68 year: 2018 end-page: 79 ident: bib0033 article-title: Pathophysiological characteristics of preproinsulin-specific CD8+ T cells in subjects with juvenile-onset and adult-onset type 1 diabetes: A 1-year follow-up study publication-title: Pediatr Diabetes – volume: 187 start-page: 1745 year: 2011 end-page: 1753 ident: bib0046 article-title: Antigen-specific TGF-beta-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity publication-title: J Immunol – volume: 157 start-page: 78 year: 2015 end-page: 90 ident: bib0034 article-title: Preproinsulin specific CD8+ T cells in subjects with latent autoimmune diabetes show lower frequency and different pathophysiological characteristics than those with type 1 diabetes publication-title: Clin Immunol – volume: 64 start-page: 916 year: 2015 end-page: 925 ident: bib0035 article-title: Beta-cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure publication-title: Diabetes – volume: 39 start-page: 612 year: 2009 end-page: 620 ident: bib0030 article-title: Functional islet-specific Treg can be generated from CD4+CD25- T cells of healthy and type 1 diabetic subjects publication-title: Eur J Immunol – volume: 27 start-page: 727 year: 2011 end-page: 736 ident: bib0025 article-title: Immunology of Diabetes Society T-Cell Workshop: HLA class II tetramer-directed epitope validation initiative publication-title: Diabetes Metab Res Rev – volume: 153 start-page: 23 year: 2014 end-page: 30 ident: bib0014 article-title: Therapy of type 1 diabetes with CD4(+)CD25(high)CD127-regulatory T cells prolongs survival of pancreatic islets - results of one year follow-up publication-title: Clin Immunol – volume: 37 start-page: 1513 year: 2014 end-page: 1520 ident: bib0010 article-title: Elevated levels of serum IL-12 and IL-18 are associated with lower frequencies of CD4(+)CD25 (high)FOXP3 (+) regulatory t cells in young patients with type 1 diabetes publication-title: Inflammation – volume: 2 year: 2012 ident: bib0001 article-title: Antigen targets of type 1 diabetes autoimmunity publication-title: Cold Spring Harb Perspect Med – volume: 35 start-page: 1817 year: 2012 end-page: 1820 ident: bib0015 article-title: Administration of CD4+CD25highCD127- regulatory T cells preserves beta-cell function in type 1 diabetes in children publication-title: Diabetes Care – volume: 7 start-page: e29355 year: 2012 ident: bib0029 article-title: Cord blood derived CD4+ CD25(high) T cells become functional regulatory T cells upon antigen encounter publication-title: PLoS One – volume: 183 start-page: 4119 year: 2009 end-page: 4126 ident: bib0045 article-title: generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid publication-title: J Immunol – volume: 181 start-page: 8209 year: 2008 end-page: 8213 ident: bib0047 article-title: Cutting edge: antigen-specific TGF beta-induced regulatory T cells suppress Th17-mediated autoimmune disease publication-title: J Immunol – volume: 10 start-page: 24 year: 2017 ident: bib0048 article-title: Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells publication-title: Epigenetics Chromatin – volume: 117 start-page: 1061 year: 2011 end-page: 1070 ident: bib0032 article-title: Infusion of publication-title: Blood – volume: 20 start-page: 624 year: 2009 end-page: 632 ident: bib0018 article-title: The regulatory function of umbilical cord blood CD4(+) CD25(+) T cells stimulated with anti-CD3/anti-CD28 and exogenous interleukin (IL)-2 or IL-15 publication-title: Pediatr Allergy Immunol – volume: 7 start-page: e29355 year: 2012 ident: bib0044 article-title: Cord blood derived CD4+ CD25high T cells become functional regulatory T cells upon antigen encounter publication-title: PloS one – volume: 25 start-page: 235 year: 2005 end-page: 243 ident: bib0050 article-title: GAD65- and proinsulin-specific CD4+ T-cells detected by MHC class II tetramers in peripheral blood of type 1 diabetes patients and at-risk subjects publication-title: J Autoimmun – volume: 71 start-page: 78 year: 2016 end-page: 87 ident: bib0006 article-title: Regulatory T cell therapy for type 1 diabetes: May the force be with you publication-title: J Autoimmun – volume: 105 start-page: 750 issue: 2 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0017 article-title: Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function publication-title: Blood doi: 10.1182/blood-2004-06-2467 – volume: 198 start-page: 729 issue: 2 year: 2017 ident: 10.1016/j.jcyt.2018.09.004_bib0013 article-title: Tolerogenic Dendritic Cells from Poorly Compensated Type 1 Diabetes Patients Have Decreased Ability To Induce Stable Antigen-Specific T Cell Hyporesponsiveness and Generation of Suppressive Regulatory T Cells publication-title: J Immunol doi: 10.4049/jimmunol.1600676 – volume: 10 start-page: 485 issue: 4 year: 1999 ident: 10.1016/j.jcyt.2018.09.004_bib0049 article-title: A kinetic basis for T cell receptor repertoire selection during an immune response publication-title: Immunity doi: 10.1016/S1074-7613(00)80048-5 – volume: 111 start-page: 453 issue: 1 year: 2008 ident: 10.1016/j.jcyt.2018.09.004_bib0023 article-title: Differential impact of mammalian target of rapamycin inhibition on CD4+CD25+Foxp3+ regulatory T cells compared with conventional CD4+ T cells publication-title: Blood doi: 10.1182/blood-2007-06-094482 – volume: 110 start-page: 211 issue: 1 year: 2007 ident: 10.1016/j.jcyt.2018.09.004_bib0039 article-title: Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper publication-title: Blood doi: 10.1182/blood-2006-10-052506 – volume: 11 start-page: 230 year: 2010 ident: 10.1016/j.jcyt.2018.09.004_bib0028 article-title: BISMA–fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences publication-title: BMC Bioinformatics doi: 10.1186/1471-2105-11-230 – volume: 105 start-page: 4743 issue: 12 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0022 article-title: Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cells publication-title: Blood doi: 10.1182/blood-2004-10-3932 – volume: 2 issue: 4 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0001 article-title: Antigen targets of type 1 diabetes autoimmunity publication-title: Cold Spring Harb Perspect Med doi: 10.1101/cshperspect.a007781 – volume: 6 start-page: 1167 issue: 25 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0026 article-title: Visualizing antigen specific CD4+ T cells using MHC class II tetramers publication-title: J Vis Exp – volume: 54 start-page: 92 issue: 1 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0011 article-title: Defective suppressor function in CD4(+)CD25(+) T-cells from patients with type 1 diabetes publication-title: Diabetes doi: 10.2337/diabetes.54.1.92 – volume: 19 start-page: 68 issue: 1 year: 2018 ident: 10.1016/j.jcyt.2018.09.004_bib0033 article-title: Pathophysiological characteristics of preproinsulin-specific CD8+ T cells in subjects with juvenile-onset and adult-onset type 1 diabetes: A 1-year follow-up study publication-title: Pediatr Diabetes doi: 10.1111/pedi.12536 – volume: 194 start-page: 113 issue: 1 year: 2015 ident: 10.1016/j.jcyt.2018.09.004_bib0031 article-title: Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region publication-title: J Immunol doi: 10.4049/jimmunol.1401145 – volume: 37 start-page: 129 issue: 1 year: 2007 ident: 10.1016/j.jcyt.2018.09.004_bib0043 article-title: Transient expression of FOXP3 in human activated nonregulatory CD4+ T cells publication-title: Eur J Immunol doi: 10.1002/eji.200636435 – volume: 93 start-page: 2302 issue: 7 year: 1999 ident: 10.1016/j.jcyt.2018.09.004_bib0041 article-title: Identification of cord blood dendritic cells as an immature CD11c- population publication-title: Blood doi: 10.1182/blood.V93.7.2302 – volume: 10 start-page: 24 year: 2017 ident: 10.1016/j.jcyt.2018.09.004_bib0048 article-title: Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells publication-title: Epigenetics Chromatin doi: 10.1186/s13072-017-0129-1 – volume: 25 start-page: 235 issue: 3 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0050 article-title: GAD65- and proinsulin-specific CD4+ T-cells detected by MHC class II tetramers in peripheral blood of type 1 diabetes patients and at-risk subjects publication-title: J Autoimmun doi: 10.1016/j.jaut.2005.09.018 – volume: 35 start-page: 1817 issue: 9 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0015 article-title: Administration of CD4+CD25highCD127- regulatory T cells preserves beta-cell function in type 1 diabetes in children publication-title: Diabetes Care doi: 10.2337/dc12-0038 – volume: 14 start-page: 322 issue: 5 year: 2013 ident: 10.1016/j.jcyt.2018.09.004_bib0016 article-title: Clinical application of regulatory T cells in type 1 diabetes publication-title: Pediatr Diabetes doi: 10.1111/pedi.12029 – volume: 20 start-page: 624 issue: 7 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0018 article-title: The regulatory function of umbilical cord blood CD4(+) CD25(+) T cells stimulated with anti-CD3/anti-CD28 and exogenous interleukin (IL)-2 or IL-15 publication-title: Pediatr Allergy Immunol doi: 10.1111/j.1399-3038.2008.00843.x – volume: 33 start-page: 1508 issue: 12 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0040 article-title: Increased induction of allogeneic-specific cord blood CD4+CD25+ regulatory T (Treg) cells: a comparative study of naive and antigenic-specific cord blood Treg cells publication-title: Exp Hematol doi: 10.1016/j.exphem.2005.09.002 – volume: 27 start-page: 727 issue: 8 year: 2011 ident: 10.1016/j.jcyt.2018.09.004_bib0025 article-title: Immunology of Diabetes Society T-Cell Workshop: HLA class II tetramer-directed epitope validation initiative publication-title: Diabetes Metab Res Rev doi: 10.1002/dmrr.1244 – volume: 187 start-page: 1745 issue: 4 year: 2011 ident: 10.1016/j.jcyt.2018.09.004_bib0046 article-title: Antigen-specific TGF-beta-induced regulatory T cells secrete chemokines, regulate T cell trafficking, and suppress ongoing autoimmunity publication-title: J Immunol doi: 10.4049/jimmunol.1004112 – volume: 19 start-page: 345 issue: 4 year: 2007 ident: 10.1016/j.jcyt.2018.09.004_bib0042 article-title: Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production publication-title: International immunology doi: 10.1093/intimm/dxm014 – volume: 181 start-page: 8209 issue: 12 year: 2008 ident: 10.1016/j.jcyt.2018.09.004_bib0047 article-title: Cutting edge: antigen-specific TGF beta-induced regulatory T cells suppress Th17-mediated autoimmune disease publication-title: J Immunol doi: 10.4049/jimmunol.181.12.8209 – volume: 12 start-page: 580 issue: 6 year: 2005 ident: 10.1016/j.jcyt.2018.09.004_bib0002 article-title: Autoimmune destruction of pancreatic β cells publication-title: American journal of therapeutics doi: 10.1097/01.mjt.0000178767.67857.63 – volume: 61 start-page: 1752 issue: 7 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0036 article-title: Circulating preproinsulin signal peptide-specific CD8 T cells restricted by the susceptibility molecule HLA-A24 are expanded at onset of type 1 diabetes and kill beta-cells publication-title: Diabetes doi: 10.2337/db11-1520 – volume: 153 start-page: 23 issue: 1 year: 2014 ident: 10.1016/j.jcyt.2018.09.004_bib0014 article-title: Therapy of type 1 diabetes with CD4(+)CD25(high)CD127-regulatory T cells prolongs survival of pancreatic islets - results of one year follow-up publication-title: Clin Immunol doi: 10.1016/j.clim.2014.03.016 – volume: 4 start-page: 178 year: 2017 ident: 10.1016/j.jcyt.2018.09.004_bib0019 article-title: Expansion of Human Tregs from Cryopreserved Umbilical Cord Blood for GMP-Compliant Autologous Adoptive Cell Transfer Therapy publication-title: Mol Ther Methods Clin Dev doi: 10.1016/j.omtm.2016.12.003 – volume: 7 start-page: e29355 issue: 1 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0029 article-title: Cord blood derived CD4+ CD25(high) T cells become functional regulatory T cells upon antigen encounter publication-title: PLoS One doi: 10.1371/journal.pone.0029355 – volume: 71 start-page: 78 year: 2016 ident: 10.1016/j.jcyt.2018.09.004_bib0006 article-title: Regulatory T cell therapy for type 1 diabetes: May the force be with you publication-title: J Autoimmun doi: 10.1016/j.jaut.2016.03.011 – volume: 7 start-page: e29355 issue: 1 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0044 article-title: Cord blood derived CD4+ CD25high T cells become functional regulatory T cells upon antigen encounter publication-title: PloS one doi: 10.1371/journal.pone.0029355 – volume: 4 start-page: e5994 issue: 6 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0021 article-title: Differential responses of human regulatory T cells (Treg) and effector T cells to rapamycin publication-title: PLoS One doi: 10.1371/journal.pone.0005994 – volume: 66 start-page: 722 issue: 3 year: 2017 ident: 10.1016/j.jcyt.2018.09.004_bib0003 article-title: Islet-Derived CD4 T Cells Targeting Proinsulin in Human Autoimmune Diabetes publication-title: Diabetes doi: 10.2337/db16-1025 – volume: 192 start-page: 303 issue: 2 year: 2000 ident: 10.1016/j.jcyt.2018.09.004_bib0004 article-title: Immunologic self-tolerance maintained by CD25+ CD4+ regulatory T cells constitutively expressing cytotoxic T lymphocyte–associated antigen 4 publication-title: The Journal of experimental medicine doi: 10.1084/jem.192.2.303 – volume: 42 start-page: 27 issue: 1 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0027 article-title: Suppression assays with human T regulatory cells: a technical guide publication-title: Eur J Immunol doi: 10.1002/eji.201141651 – volume: 32 start-page: 2041 issue: 11 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0024 article-title: Autologous umbilical cord blood transfusion in very young children with type 1 diabetes publication-title: Diabetes Care doi: 10.2337/dc09-0967 – volume: 117 start-page: 1061 issue: 3 year: 2011 ident: 10.1016/j.jcyt.2018.09.004_bib0032 article-title: Infusion of ex vivo expanded T regulatory cells in adults transplanted with umbilical cord blood: safety profile and detection kinetics publication-title: Blood doi: 10.1182/blood-2010-07-293795 – volume: 98 start-page: 1291 issue: 8 year: 2013 ident: 10.1016/j.jcyt.2018.09.004_bib0037 article-title: Differential effects of rapamycin and retinoic acid on expansion, stability and suppressive qualities of human CD4(+)CD25(+)FOXP3(+) T regulatory cell subpopulations publication-title: Haematologica doi: 10.3324/haematol.2012.074088 – volume: 60 start-page: 307 issue: 4 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0009 article-title: Low frequency of regulatory T cells in the peripheral blood of children with type 1 diabetes diagnosed under the age of five publication-title: Arch Immunol Ther Exp (Warsz) doi: 10.1007/s00005-012-0177-y – volume: 90 start-page: 617 issue: 6 year: 2011 ident: 10.1016/j.jcyt.2018.09.004_bib0020 article-title: Expansion of CD4+ CD25+ regulatory T cells from cord blood CD4+ cells using the common γ-chain cytokines (IL-2 and IL-15) and rapamycin publication-title: Annals of hematology doi: 10.1007/s00277-010-1121-z – volume: 183 start-page: 4119 issue: 6 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0045 article-title: De novo generation and enhanced suppression of human CD4+CD25+ regulatory T cells by retinoic acid publication-title: J Immunol doi: 10.4049/jimmunol.0901065 – volume: 9 start-page: 239 issue: 3 year: 2008 ident: 10.1016/j.jcyt.2018.09.004_bib0008 article-title: The Foxp3+ regulatory T cell: a jack of all trades, master of regulation publication-title: Nature immunology doi: 10.1038/ni1572 – volume: 37 start-page: 1513 issue: 5 year: 2014 ident: 10.1016/j.jcyt.2018.09.004_bib0010 article-title: Elevated levels of serum IL-12 and IL-18 are associated with lower frequencies of CD4(+)CD25 (high)FOXP3 (+) regulatory t cells in young patients with type 1 diabetes publication-title: Inflammation doi: 10.1007/s10753-014-9878-1 – volume: 173 start-page: 207 issue: 2 year: 2013 ident: 10.1016/j.jcyt.2018.09.004_bib0012 article-title: CD4(+) CD45RA(-) FoxP3high activated regulatory T cells are functionally impaired and related to residual insulin-secreting capacity in patients with type 1 diabetes publication-title: Clin Exp Immunol doi: 10.1111/cei.12116 – volume: 39 start-page: 612 issue: 2 year: 2009 ident: 10.1016/j.jcyt.2018.09.004_bib0030 article-title: Functional islet-specific Treg can be generated from CD4+CD25- T cells of healthy and type 1 diabetic subjects publication-title: Eur J Immunol doi: 10.1002/eji.200838819 – volume: 112 start-page: 1437 issue: 9 year: 2003 ident: 10.1016/j.jcyt.2018.09.004_bib0038 article-title: Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4+CD25- T cells publication-title: J Clin Invest doi: 10.1172/JCI19441 – volume: 11 start-page: 7 issue: 1 year: 2010 ident: 10.1016/j.jcyt.2018.09.004_bib0005 article-title: Regulatory T cells exert checks and balances on self tolerance and autoimmunity publication-title: Nature immunology doi: 10.1038/ni.1818 – volume: 4 start-page: 22 issue: 1 year: 2012 ident: 10.1016/j.jcyt.2018.09.004_bib0007 article-title: Induced Foxp3+ regulatory T cells: a potential new weapon to treat autoimmune and inflammatory diseases? publication-title: Journal of molecular cell biology doi: 10.1093/jmcb/mjr039 – volume: 157 start-page: 78 issue: 1 year: 2015 ident: 10.1016/j.jcyt.2018.09.004_bib0034 article-title: Preproinsulin specific CD8+ T cells in subjects with latent autoimmune diabetes show lower frequency and different pathophysiological characteristics than those with type 1 diabetes publication-title: Clin Immunol doi: 10.1016/j.clim.2015.01.005 – volume: 64 start-page: 916 issue: 3 year: 2015 ident: 10.1016/j.jcyt.2018.09.004_bib0035 article-title: Beta-cell-specific CD8 T cell phenotype in type 1 diabetes reflects chronic autoantigen exposure publication-title: Diabetes doi: 10.2337/db14-0332 |
| SSID | ssj0020885 |
| Score | 2.2583077 |
| Snippet | Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D). Cord blood (CB) can... Abstract Background Antigen-specific regulatory T cells (Tregs) have proven to be effective in reversing established autoimmunity in type 1 diabetes (T1D).... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 1355 |
| SubjectTerms | Advanced Basic Science cord blood HLA tetramers Other proinsulin regulatory T cells type 1 diabetes |
| Title | In vitro assessment of cord blood–derived proinsulin-specific regulatory T cells for cellular therapy in type 1 diabetes |
| URI | https://www.clinicalkey.com/#!/content/1-s2.0-S1465324918306133 https://www.clinicalkey.es/playcontent/1-s2.0-S1465324918306133 https://dx.doi.org/10.1016/j.jcyt.2018.09.004 https://www.ncbi.nlm.nih.gov/pubmed/30340983 https://www.proquest.com/docview/2123716039 |
| Volume | 20 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEBYhvfRS-u72EaZQcmnc9UOy7GMIDZuWhkA3kJuQLQk2FDusNwH3UPof-g_7SzpjyQ6laQq92UZCsmYkzUjfN8PYm9hYk3GeRZWo64iXxkU6rkXknCx4IUqTaiInfzrOF6f8w5k422IHIxeGYJVh7fdr-rBahy_zMJrzi9Vq_jmh0GDoPaBS0qZEET85l6Tl775NMI8UZ5HwDCMRUelAnPEYr_O6JzxlUgyxTkOyths2p78Zn8MmdHif3QvWI-z7Dj5gW7Z5yHZPfPjpfg-W12yqbg924eQ6MHX_iH09auBqtVm3oKd4nNA6IAcUBgT7z-8_DKrklTWAfQk49YjYmIQogrVPXN-ue1gCHfl3gDbv8ERoVvBkrh5WDdDRLiQwHu0-ZqeH75cHiyjkXohqnqebSEhdp7mUski0dbkxdINXiwodLiGlRqtRJ2le6cxWMXcudWjXcFMUli4is9xlT9h20zb2GQN0OGPDeZUVwnFR5OhzG1daLK-1jbWYsWQcdFWHwOSUH-OLGhFo54oEpUhQKi4VCmrG3k51LnxYjltLZ6Ms1Ug4xSVS4a5xay15Uy3bhVneqUR1qYrVH5o4Y2Kq-Zsy_7PF16OiKZzlJD3d2PayU2RgSMoIXs7YU6-B03-jEYJOepE9_89WX7C79OYJli_Z9mZ9aV-hpbWpdoaptMPu7B99XBz_Apn6Kfo |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEB7SzaG9lL67fU6h5NKY9UPy4xhCw26TLIFuIDchWxJsCHZYbwLuqf-h_7C_pBpL3lCaptCbsTVI1sxIM9J8MwAfQ6VVwlgSlLyqAlYoE8iw4oExWc5yXqhYEjj5eJ5OT9mXM362BfsDFobCKv3a79b0frX2byZ-NieXy-Xka0Spwaz3YIWSNqXkHmxTdio-gu292eF0vvG7rCJxBzLiARF47IwL8zqvOgqpjPI-3amv13bL_vQ3-7Pfhw4ewUNvQOKeG-Nj2NL1E9g5cRmou11c3ACq2l3cwZOb3NTdU_g2q_F6uV41KDcpObExSD4o9kHsP7__UFYqr7VCOxYfqh4QIJOCinDlatc3qw4XSKf-LVqzt3-igFZ0eK4OlzXS6S5GOJzuPoPTg8-L_Wngyy8EFUvjdcAzWcVplmV5JLVJlaJLvIqX1ufiWSat4SijOC1losuQGRMba9owleea7iKT1CTPYVQ3tX4JaH3OUDFWJjk3jOepdbuVKbRtL6UOJR9DNEy6qHxuciqRcSGGILRzQYwSxCgRFsIyagyfNjSXLjPHna2TgZdiwJzaVVLYjeNOquw2Kt16RW9FJNpYhOIPYRwD31D-Js__7PHDIGjCKjpxT9a6uWoF2RgZFQUvxvDCSeDmv60dYv30PHn1n72-h_vTxfGROJrND1_DA_ri8JZvYLReXem31vBal--8Yv0CtKosqw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+vitro+assessment+of+cord+blood%E2%80%93derived+proinsulin-specific+regulatory+T+cells+for+cellular+therapy+in+type+1+diabetes&rft.jtitle=Cytotherapy+%28Oxford%2C+England%29&rft.au=Paul%2C+MAHINDER&rft.au=DAYAL%2C+DEVI&rft.au=BHANSALI%2C+ANIL&rft.au=DHALIWAL%2C+LAKHBIR&rft.date=2018-11-01&rft.issn=1465-3249&rft.volume=20&rft.issue=11&rft.spage=1355&rft.epage=1370&rft_id=info:doi/10.1016%2Fj.jcyt.2018.09.004&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_j_jcyt_2018_09_004 |
| thumbnail_m | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=https%3A%2F%2Fcdn.clinicalkey.com%2Fck-thumbnails%2F14653249%2FS1465324918X00115%2Fcov150h.gif |