Phosphate-induced autophagy counteracts vascular calcification by reducing matrix vesicle release

• Published in: Kidney international Vol. 83; no. 6; pp. 1042 - 1051
• Main Authors: Dai, Xiao-Yan, Zhao, Ming-Ming, Cai, Yan, Guan, Qing-Cong, Zhao, Ying, Guan, Youfei, Kong, Wei, Zhu, Wei-Guo, Xu, Ming-Jiang, Wang, Xian
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2013; Elsevier Limited
• Subjects: Adenine - analogs & derivatives; Adenine - pharmacology; Alkaline Phosphatase - metabolism; Amino Acid Chloromethyl Ketones - pharmacology; Animals; Antioxidants - pharmacology; apoptosis; autophagy; Autophagy - drug effects; Autophagy-Related Protein 5; Caspase Inhibitors - pharmacology; Cattle; Cells, Cultured; chronic renal failure; Disease Models, Animal; hyperphosphatemia; Kidney Failure, Chronic - complications; Kidney Failure, Chronic - metabolism; Kidney Failure, Chronic - pathology; Metalloporphyrins - pharmacology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - pathology; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - metabolism; Myocytes, Smooth Muscle - pathology; oxidative stress; Phosphates - metabolism; Proteins - genetics; Proteins - metabolism; Rats; RNA Interference; Secretory Vesicles - drug effects; Secretory Vesicles - metabolism; Secretory Vesicles - pathology; Sodium-Phosphate Cotransporter Proteins, Type III - genetics; Sodium-Phosphate Cotransporter Proteins, Type III - metabolism; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Time Factors; Transfection; Valproic Acid - pharmacology; Vascular Calcification - etiology; Vascular Calcification - metabolism; Vascular Calcification - pathology; Vascular Calcification - prevention & control; apoptosis; autophagy; chronic renal failure; hyperphosphatemia; oxidative stress

Autophagy is a dynamic and highly regulated process of self-digestion responsible for cell survival and reaction to oxidative stress. As oxidative stress is increased in uremia and is associated with vascular calcification, we studied the role of autophagy in vascular calcification induced by phosph...