Evaluation of Neutralizing Antibodies Against Highly Pathogenic Coronaviruses: A Detailed Protocol for a Rapid Evaluation of Neutralizing Antibodies Using Vesicular Stomatitis Virus Pseudovirus-Based Assay

• Published in: Frontiers in microbiology Vol. 11; p. 2020
• Main Authors: Almahboub, Sarah A., Algaissi, Abdullah, Alfaleh, Mohamed A., ElAssouli, M-Zaki, Hashem, Anwar M.
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 04.09.2020
• Subjects: coronaviruses; Microbiology; Middle East respiratory syndrome coronavirus; serological assay; severe acute respiratory syndrome coronavirus 2; vesicular stomatitis virus pseudovirus; Middle East respiratory syndrome coronavirus; coronaviruses; vesicular stomatitis virus pseudovirus; severe acute respiratory syndrome coronavirus 2; serological assay

Emerging highly pathogenic human coronaviruses (CoVs) represent a serious ongoing threat to the public health worldwide. The spike (S) proteins of CoVs are surface glycoproteins that facilitate viral entry into host cells via attachment to their respective cellular receptors. The S protein is believ...