Extracellular Matrix From Decellularized Wharton's Jelly Improves the Behavior of Cells From Degenerated Intervertebral Disc
Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as a biomaterial with remarkable...
Saved in:
Published in | Frontiers in bioengineering and biotechnology Vol. 8; p. 262 |
---|---|
Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
27.03.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as a biomaterial with remarkable regenerative capacity and its potential as a therapeutic agent is rising. In the present study, we investigated the potential of decellularized Wharton's jelly matrix (DWJM) from human umbilical cord to act as an ECM-based scaffold for IVD cell culturing. An efficient detergent-enzymatic treatment (DET) was used to produce DWJM maintaining its native microarchitecture. Afterward, immunofluorescence, biochemical assays and electron microscopy analysis showed that DWJM was able to produce sizeable 3D cell aggregates, when combined with human mesenchymal stromal cells isolated from WJ (MSCs) and IVD cells. These latter cells are characterized by the loss of their chondrocyte-like phenotype since they have been isolated from degenerated IVD and
expanded to further de-differentiate. While the effect exerted by DWJM on MSCs was essentially the induction of proliferation, conversely, on IVD cells the DWJM promoted cell differentiation toward a discogenic phenotype. Notably, for the first time, the ability of DWJM to improve the degenerated phenotype of human IVD cells was demonstrated, showing that the mere presence of the matrix maintained the viability of the cells, and positively affected the expression of critical regulators of IVD homeostasis, such as SOX2, SOX9, and TRPS1 transcription factors at specific culture time. Our data are in line with the hypothesis that the strengthening of cell properties in terms of viability and expression of specific proteins at precise times represents an important condition in the perspective of guiding the recovery of cellular functionality and triggering regenerative potential. Currently, there are no definitive surgical or pharmacological treatments for IVD degeneration (IDD) able to restore the disc structure and function. Therefore, the potential of DWJM to revert degenerated IVD cells could be exploited in the next future an ECM-based intradiscal injectable therapeutic. |
---|---|
AbstractList | Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as a biomaterial with remarkable regenerative capacity and its potential as a therapeutic agent is rising. In the present study, we investigated the potential of decellularized Wharton's jelly matrix (DWJM) from human umbilical cord to act as an ECM-based scaffold for IVD cell culturing. An efficient detergent-enzymatic treatment (DET) was used to produce DWJM maintaining its native microarchitecture. Afterward, immunofluorescence, biochemical assays and electron microscopy analysis showed that DWJM was able to produce sizeable 3D cell aggregates, when combined with human mesenchymal stromal cells isolated from WJ (MSCs) and IVD cells. These latter cells are characterized by the loss of their chondrocyte-like phenotype since they have been isolated from degenerated IVD and
expanded to further de-differentiate. While the effect exerted by DWJM on MSCs was essentially the induction of proliferation, conversely, on IVD cells the DWJM promoted cell differentiation toward a discogenic phenotype. Notably, for the first time, the ability of DWJM to improve the degenerated phenotype of human IVD cells was demonstrated, showing that the mere presence of the matrix maintained the viability of the cells, and positively affected the expression of critical regulators of IVD homeostasis, such as SOX2, SOX9, and TRPS1 transcription factors at specific culture time. Our data are in line with the hypothesis that the strengthening of cell properties in terms of viability and expression of specific proteins at precise times represents an important condition in the perspective of guiding the recovery of cellular functionality and triggering regenerative potential. Currently, there are no definitive surgical or pharmacological treatments for IVD degeneration (IDD) able to restore the disc structure and function. Therefore, the potential of DWJM to revert degenerated IVD cells could be exploited in the next future an ECM-based intradiscal injectable therapeutic. Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as a biomaterial with remarkable regenerative capacity and its potential as a therapeutic agent is rising. In the present study, we investigated the potential of decellularized Wharton’s jelly matrix (DWJM) from human umbilical cord to act as an ECM-based scaffold for IVD cell culturing. An efficient detergent-enzymatic treatment (DET) was used to produce DWJM maintaining its native microarchitecture. Afterward, immunofluorescence, biochemical assays and electron microscopy analysis showed that DWJM was able to produce sizeable 3D cell aggregates, when combined with human mesenchymal stromal cells isolated from WJ (MSCs) and IVD cells. These latter cells are characterized by the loss of their chondrocyte-like phenotype since they have been isolated from degenerated IVD and in vitro expanded to further de-differentiate. While the effect exerted by DWJM on MSCs was essentially the induction of proliferation, conversely, on IVD cells the DWJM promoted cell differentiation toward a discogenic phenotype. Notably, for the first time, the ability of DWJM to improve the degenerated phenotype of human IVD cells was demonstrated, showing that the mere presence of the matrix maintained the viability of the cells, and positively affected the expression of critical regulators of IVD homeostasis, such as SOX2, SOX9, and TRPS1 transcription factors at specific culture time. Our data are in line with the hypothesis that the strengthening of cell properties in terms of viability and expression of specific proteins at precise times represents an important condition in the perspective of guiding the recovery of cellular functionality and triggering regenerative potential. Currently, there are no definitive surgical or pharmacological treatments for IVD degeneration (IDD) able to restore the disc structure and function. Therefore, the potential of DWJM to revert degenerated IVD cells could be exploited in the next future an ECM-based intradiscal injectable therapeutic. Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this field. Extracellular matrix (ECM) deriving from decellularized non-autologous tissues has been established as a biomaterial with remarkable regenerative capacity and its potential as a therapeutic agent is rising. In the present study, we investigated the potential of decellularized Wharton’s jelly matrix (DWJM) from human umbilical cord to act as an ECM-based scaffold for IVD cell culturing. An efficient detergent-enzymatic treatment (DET) was used to produce DWJM maintaining its native microarchitecture. Afterward, immunofluorescence, biochemical assays and electron microscopy analysis showed that DWJM was able to produce sizeable 3D cell aggregates, when combined with human mesenchymal stromal cells isolated from WJ (MSCs) and IVD cells. These latter cells are characterized by the loss of their chondrocyte-like phenotype since they have been isolated from degenerated IVD and in vitro expanded to further de-differentiate. While the effect exerted by DWJM on MSCs was essentially the induction of proliferation, conversely, on IVD cells the DWJM promoted cell differentiation toward a discogenic phenotype. Notably, for the first time, the ability of DWJM to improve the degenerated phenotype of human IVD cells was demonstrated, showing that the mere presence of the matrix maintained the viability of the cells, and positively affected the expression of critical regulators of IVD homeostasis, such as SOX2, SOX9, and TRPS1 transcription factors at specific culture time. Our data are in line with the hypothesis that the strengthening of cell properties in terms of viability and expression of specific proteins at precise times represents an important condition in the perspective of guiding the recovery of cellular functionality and triggering regenerative potential. Currently, there are no definitive surgical or pharmacological treatments for IVD degeneration (IDD) able to restore the disc structure and function. Therefore, the potential of DWJM to revert degenerated IVD cells could be exploited in the next future an ECM-based intradiscal injectable therapeutic. |
Author | De Bonis, Pasquale Lambertini, Elisabetta Piva, Roberta Penolazzi, Letizia Bonaccorsi, Gloria D'Agostino, Stefania Francescato, Riccardo Cavallo, Michele Pozzobon, Michela Bergamin, Leticia Scussel |
AuthorAffiliation | 4 Section of Obstetrics and Gynecology, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, and S. Anna University Hospital , Ferrara , Italy 2 Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza , Padua , Italy 3 Department of Women and Children Health, University of Padova , Padua , Italy 1 Department of Biomedical and Specialty Surgical Sciences, University of Ferrara , Ferrara , Italy 5 Department of Neurosurgery, University of Ferrara, and S. Anna University Hospital , Ferrara , Italy |
AuthorAffiliation_xml | – name: 3 Department of Women and Children Health, University of Padova , Padua , Italy – name: 2 Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza , Padua , Italy – name: 1 Department of Biomedical and Specialty Surgical Sciences, University of Ferrara , Ferrara , Italy – name: 4 Section of Obstetrics and Gynecology, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, and S. Anna University Hospital , Ferrara , Italy – name: 5 Department of Neurosurgery, University of Ferrara, and S. Anna University Hospital , Ferrara , Italy |
Author_xml | – sequence: 1 givenname: Letizia surname: Penolazzi fullname: Penolazzi, Letizia organization: Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy – sequence: 2 givenname: Michela surname: Pozzobon fullname: Pozzobon, Michela organization: Department of Women and Children Health, University of Padova, Padua, Italy – sequence: 3 givenname: Leticia Scussel surname: Bergamin fullname: Bergamin, Leticia Scussel organization: Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy – sequence: 4 givenname: Stefania surname: D'Agostino fullname: D'Agostino, Stefania organization: Department of Women and Children Health, University of Padova, Padua, Italy – sequence: 5 givenname: Riccardo surname: Francescato fullname: Francescato, Riccardo organization: Stem Cells and Regenerative Medicine Lab, Fondazione Istituto di Ricerca Pediatrica Città della Speranza, Padua, Italy – sequence: 6 givenname: Gloria surname: Bonaccorsi fullname: Bonaccorsi, Gloria organization: Section of Obstetrics and Gynecology, Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, and S. Anna University Hospital, Ferrara, Italy – sequence: 7 givenname: Pasquale surname: De Bonis fullname: De Bonis, Pasquale organization: Department of Neurosurgery, University of Ferrara, and S. Anna University Hospital, Ferrara, Italy – sequence: 8 givenname: Michele surname: Cavallo fullname: Cavallo, Michele organization: Department of Neurosurgery, University of Ferrara, and S. Anna University Hospital, Ferrara, Italy – sequence: 9 givenname: Elisabetta surname: Lambertini fullname: Lambertini, Elisabetta organization: Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy – sequence: 10 givenname: Roberta surname: Piva fullname: Piva, Roberta organization: Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32292779$$D View this record in MEDLINE/PubMed |
BookMark | eNpVkk1PGzEQhq2KqlDg3lPlW7kk9dfuei-VSoA2FVUvVD1a_hgnRrvr1N5EgPrjaxKC4GRr3plnxuP3PToY4gAIfaBkyrlsP3sTIkwZYWRKCKvZG3TEWFtPBJXVwYv7ITrN-ZYQQlnVVJK9Q4e8iKxp2iP07_JuTNpC1607nfBPPaZwh69S7PEF7MPhARz-s9RpjMOnjH-U8D2e96sUN5DxuAR8Dku9CTHh6PGsyHmPWMAASY-lfj6MkDaQRjBJd_giZHuC3nrdZTh9Oo_R76vLm9n3yfWvb_PZ1-uJFTUbJ5xKT6WkhtVgqa2opd54zsFxKXhVsUqAIdIZ4zTxUhornaPOOyMMay3hx2i-47qob9UqhV6nexV1UNtATAtV3hZsB6o0Yq1kkjsAUbeN4dBqqrkRTLS6hsL6smOt1qYHZ2Eo--teQV8rQ1iqRdyohhY0EQVw9gRI8e8a8qj6soqyMz1AXGfFeEtoTWpWlVSyS7Up5pzAP7ehRD16QG09oB49oLYeKCUfX473XLD_cf4fX7-ynQ |
CitedBy_id | crossref_primary_10_3389_fbioe_2022_828424 crossref_primary_10_1016_j_apmt_2024_102241 crossref_primary_10_1016_j_mtchem_2024_102145 crossref_primary_10_1016_j_bioactmat_2024_01_004 crossref_primary_10_15825_1995_1191_2023_2_82_98 crossref_primary_10_1016_j_lfs_2022_120623 crossref_primary_10_1002_jsp2_1117 crossref_primary_10_3390_biomedicines10020227 crossref_primary_10_1155_2022_2454168 crossref_primary_10_3390_app11041919 crossref_primary_10_3390_ijms23136915 crossref_primary_10_1016_j_mtbio_2022_100523 crossref_primary_10_3390_biomimetics9070405 crossref_primary_10_3390_ph14111090 crossref_primary_10_1016_j_ijbiomac_2023_127686 crossref_primary_10_3389_fbioe_2022_961987 crossref_primary_10_1002_jbm_a_37683 crossref_primary_10_3390_jfb14020104 crossref_primary_10_3389_fcell_2024_1368318 crossref_primary_10_1039_D2BM01977A crossref_primary_10_3389_fbioe_2022_968862 crossref_primary_10_3389_fbioe_2022_884069 crossref_primary_10_1186_s13287_022_03009_5 crossref_primary_10_1152_ajpcell_00178_2023 crossref_primary_10_1016_j_bonr_2024_101784 crossref_primary_10_3390_gels10010062 |
Cites_doi | 10.1155/2014/762189 10.1089/ten.TEA.2018.0297 10.3389/fbioe.2016.00093 10.1016/s0955-0674(02)00364-362 10.1016/j.actbio.2018.09.044 10.18632/oncotarget.7735 10.1007/s12178-017-9441-4 10.1371/journal.pone.0093570 10.3390/polym11071151 10.1016/j.biomaterials.2015.10.005 10.1016/j.msec.2019.02.045 10.1038/ncomms15613 10.1002/jsp2.1029 10.1002/jbm.a.36434 10.1007/978-3-319-37076-7_10-1 10.1016/j.spinee.2012.02.027 10.1371/journal.pone.0172098 10.1111/j.1365-2184.2010.00729.x 10.1002/jbm.b.34196 10.1007/s11010-018-3297-9 10.1126/scitranslmed.aau0670 10.1089/ten.TEC.2008.0582 10.1177/1947603519876354 10.1016/j.exger.2016.07.008 10.1016/j.msec.2017.04.074 10.18632/aging.101525 10.1016/j.spinee.2016.10.022 10.1097/BRS.0b013e3181d0c944 10.1038/nrrheum.2015.13 10.1002/jcp.22983 10.1186/s40824-017-0106-6 10.3390/cells8101170 10.1002/jor.23088 10.1182/bloodadvances.2018019315 10.3390/ijms20153622 10.1007/s40204-019-0108-7 10.1016/j.actbio.2018.08.004 10.1002/jbm.a.36759 10.22203/ecm.v020a25 10.1016/0304-4165(86)90306-5 10.3390/ijms19124117 10.1038/s41536-018-0057-0 10.1002/jcb.27965 10.1089/ten.TEA.2015.0422 10.1016/j.ejogrb.2009.02.024 10.1016/S0140-6736(12)61689-4 10.1097/01.brs.0000146101.53784.b1 10.1016/j.biomaterials.2011.01.057 10.1097/01.BRS.0000058946.64222.92 10.1096/fj.201801332R 10.1007/s00586-007-0414-y 10.1002/jbm4.10180 10.1155/2018/4696180 |
ContentType | Journal Article |
Copyright | Copyright © 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva. Copyright © 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva. 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva |
Copyright_xml | – notice: Copyright © 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva. – notice: Copyright © 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva. 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva |
DBID | NPM AAYXX CITATION 7X8 5PM DOA |
DOI | 10.3389/fbioe.2020.00262 |
DatabaseName | PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals |
DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
DatabaseTitleList | PubMed |
Database_xml | – sequence: 1 dbid: DOA name: Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering |
EISSN | 2296-4185 |
EndPage | 262 |
ExternalDocumentID | oai_doaj_org_article_f18298283dee4697b3e9a1a3b4249a6e 10_3389_fbioe_2020_00262 32292779 |
Genre | Journal Article |
GroupedDBID | 53G 5VS 9T4 AAFWJ ACGFS ACXDI ADBBV ADRAZ ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BCNDV DIK GROUPED_DOAJ GX1 HYE IAO IEA IHR IPNFZ ISR KQ8 M48 M~E NPM OK1 PGMZT RIG RPM AAYXX AFPKN CITATION 7X8 5PM |
ID | FETCH-LOGICAL-c462t-318f1881b26ec1c51c1fbf33ed384355254eb08dbbda0f88bc8dd1dfdb4b29c03 |
IEDL.DBID | RPM |
ISSN | 2296-4185 |
IngestDate | Tue Oct 22 15:13:51 EDT 2024 Tue Sep 17 21:13:45 EDT 2024 Sat Oct 05 05:28:34 EDT 2024 Thu Sep 26 16:04:29 EDT 2024 Wed Oct 16 00:46:38 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | intervertebral disc cells Wharton’s jelly scaffold TRPS1 decellularized matrix |
Language | English |
License | Copyright © 2020 Penolazzi, Pozzobon, Bergamin, D’Agostino, Francescato, Bonaccorsi, De Bonis, Cavallo, Lambertini and Piva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c462t-318f1881b26ec1c51c1fbf33ed384355254eb08dbbda0f88bc8dd1dfdb4b29c03 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Sibylle Grad, AO Foundation, Switzerland; Silvia Lopa, Istituto Ortopedico Galeazzi (IRCCS), Italy Edited by: Dimitrios I. Zeugolis, National University of Ireland Galway, Ireland These authors have contributed equally to this work This article was submitted to Tissue Engineering and Regenerative Medicine, a section of the journal Frontiers in Bioengineering and Biotechnology |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7118204/ |
PMID | 32292779 |
PQID | 2390160625 |
PQPubID | 23479 |
PageCount | 1 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_f18298283dee4697b3e9a1a3b4249a6e pubmedcentral_primary_oai_pubmedcentral_nih_gov_7118204 proquest_miscellaneous_2390160625 crossref_primary_10_3389_fbioe_2020_00262 pubmed_primary_32292779 |
PublicationCentury | 2000 |
PublicationDate | 2020-03-27 |
PublicationDateYYYYMMDD | 2020-03-27 |
PublicationDate_xml | – month: 03 year: 2020 text: 2020-03-27 day: 27 |
PublicationDecade | 2020 |
PublicationPlace | Switzerland |
PublicationPlace_xml | – name: Switzerland |
PublicationTitle | Frontiers in bioengineering and biotechnology |
PublicationTitleAlternate | Front Bioeng Biotechnol |
PublicationYear | 2020 |
Publisher | Frontiers Media S.A |
Publisher_xml | – name: Frontiers Media S.A |
References | Coogan (B7) 2016; 4 Hensley (B18) 2018; 106 Kim (B22) 2016; 83 Alini (B1) 2008; 17 Tukmachev (B45) 2016; 22 Paul (B30) 2003; 28 D’Este (B10) 2018; 15 Illien-Jünger (B19) 2016; 34 Beiki (B4) 2017; 78 Forraz (B14) 2011; 44 Murray (B27) 2012; 380 Ventre (B47) 2019; 107 Walker (B50) 2019 Quarta (B37) 2017; 8 Vo (B48) 2013; 13 Stocco (B42) 2014; 2014 Schizas (B41) 2010; 20 Kehtari (B21) 2019; 120 Sakai (B40) 2015; 11 Buckley (B5) 2018; 1 Van Uden (B46) 2017; 21 Wachs (B49) 2017; 17 Ferguson (B13) 2009; 144 Piccoli (B35) 2016; 74 Cukierman (B9) 2002; 14 Gullbrand (B17) 2018; 10 Penolazzi (B33) 2012; 227 Jadalannagari (B20) 2017; 12 Penolazzi (B32) 2019; 8 Crapo (B8) 2011; 32 Sun (B43) 2014; 9 Amin (B2) 2017; 10 Li (B23) 2019; 3 Zhao (B52) 2019; 11 Quarta (B38) 2018; 3 Tendulkar (B44) 2019; 20 Farndale (B11) 1986; 883 Porzionato (B36) 2018; 19 Roughley (B39) 2004; 29 Najar (B28) 2018; 447 Fujii (B15) 2019; 3 Lin (B24) 2016; 7 Liu (B25) 2019; 25 Farrugia (B12) 2019 Zhou (B53) 2018; 81 Ohtori (B29) 2011; 36 Xu (B51) 2019; 99 Bullard (B6) 2019; 107 Geurts (B16) 2018; 2018 McNeill (B26) 2019; 33 Basiri (B3) 2019; 8 Penolazzi (B34) 2010; 16 Penolazzi (B31) 2018; 10 |
References_xml | – volume: 2014 year: 2014 ident: B42 article-title: Tailored PVA/ECM scaffolds for cartilage regeneration. publication-title: Biomed. Res. Int. doi: 10.1155/2014/762189 contributor: fullname: Stocco – volume: 25 start-page: 1605 year: 2019 ident: B25 article-title: Decellularized annulus fibrosus matrix/chitosan hybrid hydrogels with basic fibroblast growth factor for annulus fibrosus tissue engineering. publication-title: Tissue Eng Part A doi: 10.1089/ten.TEA.2018.0297 contributor: fullname: Liu – volume: 4 year: 2016 ident: B7 article-title: Finite element study of a lumbar intervertebral disc nucleus replacement device. publication-title: Front. Bioeng. Biotechnol. doi: 10.3389/fbioe.2016.00093 contributor: fullname: Coogan – volume: 14 start-page: 633 year: 2002 ident: B9 article-title: Cell interactions with three-dimensional matrices. publication-title: Curr. Opin. Cell Biol. doi: 10.1016/s0955-0674(02)00364-362 contributor: fullname: Cukierman – volume: 81 start-page: 115 year: 2018 ident: B53 article-title: Injectable decellularized nucleus pulposus-based cell delivery system for differentiation of adipose-derived stem cells and nucleus pulposus regereration. publication-title: Acta Biomat. doi: 10.1016/j.actbio.2018.09.044 contributor: fullname: Zhou – volume: 7 start-page: 12121 year: 2016 ident: B24 article-title: Decellularized allogeneic intervertebral disc: natural biomaterials for regenerating disc degeneration. publication-title: Oncotarget doi: 10.18632/oncotarget.7735 contributor: fullname: Lin – volume: 10 start-page: 507 year: 2017 ident: B2 article-title: Lumbar disc herniation. publication-title: Curr. Rev. Musculoskelet. Med. doi: 10.1007/s12178-017-9441-4 contributor: fullname: Amin – volume: 9 year: 2014 ident: B43 article-title: Sox9 gene transfer enhanced regenerative effect of bone marrow mesenchymal stem cells on the degenerated intervertebral Disc in a rabbit model. publication-title: PLoS One doi: 10.1371/journal.pone.0093570 contributor: fullname: Sun – volume: 11 year: 2019 ident: B52 article-title: Disordered mechanical stress and tissue engineering therapies in intervertebral disc degeneration. publication-title: Polymers doi: 10.3390/polym11071151 contributor: fullname: Zhao – volume: 74 start-page: 245 year: 2016 ident: B35 article-title: Improvement of diaphragmatic performance through orthotopic application of decellularized extracellular matrix patch. publication-title: Biomaterials doi: 10.1016/j.biomaterials.2015.10.005 contributor: fullname: Piccoli – volume: 99 start-page: 1213 year: 2019 ident: B51 article-title: Decellularised nucleus pulposus as a potential biologic scaffold for disc tissue engineering. publication-title: Mater. Sci. Eng. C doi: 10.1016/j.msec.2019.02.045 contributor: fullname: Xu – volume: 8 year: 2017 ident: B37 article-title: Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss. publication-title: Nat. Commun. doi: 10.1038/ncomms15613 contributor: fullname: Quarta – volume: 1 year: 2018 ident: B5 article-title: Critical aspects and challenges for intervertebral disc repair and regeneration—Harnessing advances in tissue engineering. publication-title: JOR Spine doi: 10.1002/jsp2.1029 contributor: fullname: Buckley – volume: 106 start-page: 2412 year: 2018 ident: B18 article-title: Decellularization and characterization of a whole intervertebral disk xenograft scaffold. publication-title: J. Biomed. Mater. Res. A doi: 10.1002/jbm.a.36434 contributor: fullname: Hensley – year: 2019 ident: B50 article-title: Stem cells: umbilical cord/wharton’s jelly derived publication-title: Cell Engineering and Regeneration. Reference Series in Biomedical Engineering. doi: 10.1007/978-3-319-37076-7_10-1 contributor: fullname: Walker – volume: 13 start-page: 331 year: 2013 ident: B48 article-title: Expression and regulation of metalloproteinases and their inhibitors in intervertebral disc aging and degeneration. publication-title: Spine J. doi: 10.1016/j.spinee.2012.02.027 contributor: fullname: Vo – volume: 12 year: 2017 ident: B20 article-title: Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications. publication-title: PLoS One doi: 10.1371/journal.pone.0172098 contributor: fullname: Jadalannagari – volume: 44 start-page: 60 year: 2011 ident: B14 article-title: The umbilical cord: a rich and ethical stem cell source to advance regenerative medicine. publication-title: Cell Prolif. doi: 10.1111/j.1365-2184.2010.00729.x contributor: fullname: Forraz – volume: 107 start-page: 1035 year: 2019 ident: B6 article-title: Evaluation of dehydrated human umbilical cord biological properties for wound care and soft tissue healing. publication-title: J. Biomed. Mater. Res. B Appl.Biomater. doi: 10.1002/jbm.b.34196 contributor: fullname: Bullard – volume: 447 start-page: 111 year: 2018 ident: B28 article-title: Immunological impact of Wharton’s Jelly mesenchymal stromal cells and natural killer cell co-culture. publication-title: Mol. Cell Biochem. doi: 10.1007/s11010-018-3297-9 contributor: fullname: Najar – volume: 10 year: 2018 ident: B17 article-title: Long-term mechanical function and integration of an implanted tissue-engineered intervertebral disc. publication-title: Sci. Transl. Med. doi: 10.1126/scitranslmed.aau0670 contributor: fullname: Gullbrand – volume: 16 start-page: 141 year: 2010 ident: B34 article-title: Encapsulation of mesenchymal stem cells from Wharton’s jelly in alginate microbeads. publication-title: Tissue Eng. C Methods doi: 10.1089/ten.TEC.2008.0582 contributor: fullname: Penolazzi – year: 2019 ident: B12 article-title: Spatiotemporal expression of 3-B-3(-) and 7-D-4 chondroitin sulfation, tissue remodeling, and attempted repair in an ovine model of intervertebral disc degeneration. publication-title: Cartilage doi: 10.1177/1947603519876354 contributor: fullname: Farrugia – volume: 83 start-page: 37 year: 2016 ident: B22 article-title: Recovery from volumetric muscle loss injury: a comparison between young and aged rats. publication-title: Exp. Gerontol. doi: 10.1016/j.exger.2016.07.008 contributor: fullname: Kim – volume: 78 start-page: 627 year: 2017 ident: B4 article-title: Fabrication of a three dimensional spongy scaffold using human Wharton’s jelly derived extra cellular matrix for wound healing. publication-title: Mater. Sci. Eng. C Mater. Biol. Appl. doi: 10.1016/j.msec.2017.04.074 contributor: fullname: Beiki – volume: 10 start-page: 2001 year: 2018 ident: B31 article-title: MicroRNA-221 silencing attenuates the degenerated phenotype of intervertebral disc cells. publication-title: Aging doi: 10.18632/aging.101525 contributor: fullname: Penolazzi – volume: 17 start-page: 435 year: 2017 ident: B49 article-title: Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering. publication-title: Spine J. doi: 10.1016/j.spinee.2016.10.022 contributor: fullname: Wachs – volume: 36 start-page: 347 year: 2011 ident: B29 article-title: Surgical versus nonsurgical treatment of selected patients with discogenic low back pain: a small-sized randomized trial. publication-title: Spine doi: 10.1097/BRS.0b013e3181d0c944 contributor: fullname: Ohtori – volume: 11 start-page: 243 year: 2015 ident: B40 article-title: Stem cell therapy for intervertebral disc regeneration: obstacles and solutions. publication-title: Nat. Rev. Rheumatol. doi: 10.1038/nrrheum.2015.13 contributor: fullname: Sakai – volume: 227 start-page: 857 year: 2012 ident: B33 article-title: Human mesenchymal stem cells seeded on extracellular matrix-scaffold: viability and osteogenic potential. publication-title: J. Cell. Physiol. doi: 10.1002/jcp.22983 contributor: fullname: Penolazzi – volume: 21 year: 2017 ident: B46 article-title: Current strategies for treatment of intervertebral disc degeneration: substitution and regeneration possibilities. publication-title: Biomater. Res. doi: 10.1186/s40824-017-0106-6 contributor: fullname: Van Uden – volume: 8 year: 2019 ident: B32 article-title: Reciprocal regulation of TRPS1 and miR-221 in intervertebral disc cells. publication-title: Cells doi: 10.3390/cells8101170 contributor: fullname: Penolazzi – volume: 34 start-page: 876 year: 2016 ident: B19 article-title: Development of a bovine decellularized extracellular matrix-biomaterial for nucleus pulposus regeneration. publication-title: J. Orthop. Res. doi: 10.1002/jor.23088 contributor: fullname: Illien-Jünger – volume: 3 start-page: 1011 year: 2019 ident: B23 article-title: Decellularized Wharton’s jelly matrix: a biomimetic scaffold for ex vivo hematopoietic stem cell culture. publication-title: Blood Adv. doi: 10.1182/bloodadvances.2018019315 contributor: fullname: Li – volume: 20 year: 2019 ident: B44 article-title: Intervertebral disc nucleus repair: hype or hope? publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms20153622 contributor: fullname: Tendulkar – volume: 8 start-page: 31 year: 2019 ident: B3 article-title: A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering. publication-title: Prog. Biomater. doi: 10.1007/s40204-019-0108-7 contributor: fullname: Basiri – volume: 15 start-page: 13 year: 2018 ident: B10 article-title: Lessons to be learned and future directions for intervertebral disc biomaterials. publication-title: Acta Biomater. doi: 10.1016/j.actbio.2018.08.004 contributor: fullname: D’Este – volume: 107 start-page: 2536 year: 2019 ident: B47 article-title: Alligned fibrous decellularized cell derived matrices for mesenchymal stem cell amplification. publication-title: J. Biomed. Mater. Res. A doi: 10.1002/jbm.a.36759 contributor: fullname: Ventre – volume: 20 start-page: 306 year: 2010 ident: B41 article-title: Disc degeneration: current surgical options. publication-title: Eur. Cells Mater. doi: 10.22203/ecm.v020a25 contributor: fullname: Schizas – volume: 883 start-page: 173 year: 1986 ident: B11 article-title: Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. publication-title: Biochim. Biophys. Acta doi: 10.1016/0304-4165(86)90306-5 contributor: fullname: Farndale – volume: 19 year: 2018 ident: B36 article-title: Tissue-engineered grafts from human decellularized extracellular matrices: a systematic review and future perspectives. publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms19124117 contributor: fullname: Porzionato – volume: 3 year: 2018 ident: B38 article-title: Biomechanics show stem cell necessity for effective treatment of volumetric muscle loss using bioengineered constructs. publication-title: NPJ Regen. Med. doi: 10.1038/s41536-018-0057-0 contributor: fullname: Quarta – volume: 120 start-page: 6683 year: 2019 ident: B21 article-title: Decellularized Wharton’s jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells. publication-title: J. Cell. Biochem. doi: 10.1002/jcb.27965 contributor: fullname: Kehtari – volume: 22 start-page: 306 year: 2016 ident: B45 article-title: Injectable extracellular matrix hydrogels as scaffolds for spinal cord injury repair. publication-title: Tissue Eng. A doi: 10.1089/ten.TEA.2015.0422 contributor: fullname: Tukmachev – volume: 144 start-page: S108 year: 2009 ident: B13 article-title: Bioengineering aspects of the umbilical cord. publication-title: Eur. J. Obstet. Gynecol. Reprod. Biol. doi: 10.1016/j.ejogrb.2009.02.024 contributor: fullname: Ferguson – volume: 380 start-page: 2197 year: 2012 ident: B27 article-title: Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. publication-title: Lancet doi: 10.1016/S0140-6736(12)61689-4 contributor: fullname: Murray – volume: 29 start-page: 2691 year: 2004 ident: B39 article-title: Biology of intervertebral disc aging and degeneration: involvement of the extracellular matrix. publication-title: Spine doi: 10.1097/01.brs.0000146101.53784.b1 contributor: fullname: Roughley – volume: 32 start-page: 3233 year: 2011 ident: B8 article-title: An overview of tissue and whole organ decellularization processes. publication-title: Biomaterials doi: 10.1016/j.biomaterials.2011.01.057 contributor: fullname: Crapo – volume: 28 start-page: 755 year: 2003 ident: B30 article-title: Potential use of Sox9 gene therapy for intervertebral degenerative disc disease. publication-title: Spine doi: 10.1097/01.BRS.0000058946.64222.92 contributor: fullname: Paul – volume: 33 start-page: 4166 year: 2019 ident: B26 article-title: Collagen biomaterial stimulates the production of extracellular vesicles containing microRNA-21 and enhances the proangiogenic function of CD34+ cells. publication-title: FASEB J. doi: 10.1096/fj.201801332R contributor: fullname: McNeill – volume: 17 start-page: 2 year: 2008 ident: B1 article-title: Are animal models useful for studying human disc disorders/degeneration? publication-title: Eur. Spine J. doi: 10.1007/s00586-007-0414-y contributor: fullname: Alini – volume: 3 year: 2019 ident: B15 article-title: Discogenic back pain: literature review of definition, diagnosis, and treatment. publication-title: JBMR Plus doi: 10.1002/jbm4.10180 contributor: fullname: Fujii – volume: 2018 start-page: 1 year: 2018 ident: B16 article-title: The impact of chronic discogenic low back pain: costs and patients’ burden. publication-title: Pain Res. Manag. doi: 10.1155/2018/4696180 contributor: fullname: Geurts |
SSID | ssj0001257582 |
Score | 2.327188 |
Snippet | Regenerative therapies for intervertebral disc (IVD) injuries are currently a major challenge that is addressed in different ways by scientists working in this... |
SourceID | doaj pubmedcentral proquest crossref pubmed |
SourceType | Open Website Open Access Repository Aggregation Database Index Database |
StartPage | 262 |
SubjectTerms | Bioengineering and Biotechnology decellularized matrix intervertebral disc cells scaffold TRPS1 Wharton’s jelly |
SummonAdditionalLinks | – databaseName: Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3LbtQwFLVQV7BAvAkvGYkNi6jxI4mzhLajUqmsqOjOiu1rOhIk1WQqlS4qfoPf40u4156pZhASG7ZxHs49jn2OfXPM2ButtRNdlIgAQKmjr0pXRShV70LjkaCaZNd0_LE5PNFHp_XpxlZflBOW7YFz4HYjEuAOZYEKACjlWqeg60WvnEbh0DeQel9Rb4ipPLuCNMTIvC6JKqzbjW4-ki2mpFQu2citcSjZ9f-NY_6ZKrkx9szusbsr0sjf5creZ7dgeMDubFgJPmTXB5d4JU3DU14pPybn_Us-W4zf-D6sD8-vIPDPZ_jS4_Drx8-JH2HBd54nFmDiSAb5yjBxwcfI97B4Wt_kSzKoRoLKP-Q8ycWSFp2_8v355B-xk9nBp73DcrW3Qul1I5f00zRGFjmrbMALXwsvootKQVAGGVSNuhFcZYJzoa-iMc6bEESIwWknO1-px2xnGAd4yjj2EY13NQ62IZIaNUKoiColah1805uCvV1H2p5nCw2L0oNQsQkVS6jYhErB3hMUN-eR-XU6gE3CrpqE_VeTKNjrNZAWPxaKcT_AeDFZSTM8KNlkXbAnGdibR2HP1sm27QrWbkG-VZftkmF-lgy5W1JplX72Pyr_nN2mcFCam2xfsJ3l4gJeIu9Zulepif8GUXsFfw priority: 102 providerName: Directory of Open Access Journals – databaseName: Scholars Portal Open Access Journals dbid: M48 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Jb9QwGLVQucABtayBgoyEhDgE4iWJc6gQtB2VSsOJEb1Z8daO1CY0mUpTxI_v9zmZ0kFz4Gpn9fPynv35mZB3UkrDqsABAe9TGWyWmiz4VNTGFRYIqop2TdPvxdFMHp_kJ3-3R48F2G-Udnie1Kw7_7i8vP4MDX4PFSeMt5-CmbfoeMkxSotjh3yfSyGxvk9Hsj_MuAA1iadHcV4VKbq2DOuWGx-yNk5FO_9NHPTfUMo7Y9NkmzwaSSX9MtSCHXLPN4_JwztWg0_In8Ml3InT9Bh3SqfozL-kk669oAd-lTz_7R39eQa1qW3e9_QYkq_pMO3gewpUkY52ih1tA92H7H71iNNoXw30lX4boii7BS5Jn9ODeW-fktnk8Mf-UTqevJBaWfAFbqkOTAGj5YW3zObMsmCCEN4JBfwqB1XpTaacMa7OglLGKueYC85IwyubiWdkq2kb_4JQ6EEKa3IYil1AraoYEwE0TJDS2aJWCfmwKmf9azDY0CBMEBMdMdGIiY6YJOQrAnF7HVpjx4S2O9VjS9Pw4bwCHSmc96D9SyN8VbNaGAlKsy58Qt6uYNTQlLCE68a3V73mOP8Dgo7nCXk-wHr7Kuj3Kl6WVULKNcDXvmU9p5mfRbvuEjVcJl_-x3tfkQf4txjjxstdsrXorvxrID0L8ybW5Ru9hwLF priority: 102 providerName: Scholars Portal |
Title | Extracellular Matrix From Decellularized Wharton's Jelly Improves the Behavior of Cells From Degenerated Intervertebral Disc |
URI | https://www.ncbi.nlm.nih.gov/pubmed/32292779 https://search.proquest.com/docview/2390160625 https://pubmed.ncbi.nlm.nih.gov/PMC7118204 https://doaj.org/article/f18298283dee4697b3e9a1a3b4249a6e |
Volume | 8 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LbtQwFLXarmCBeJMClZHYsEgnsZ3EWcK0o1JpEAsqurPiVxupk1TJVCosEL_B7_El3Osk1QxixSaLOA_H9yY-5-bkhJC3Qgidlp5BBJyLhTdJrBPvYl5pmxsAqDLYNS0_5Sdn4vQ8O98h2fQtTBDtG10fNlerw6a-DNrK65WZTTqx2eflvEBUnIjZLtktON-g6ENhBRCIZMMrSSBg5czrukVHTIYqLpbjD2wgjUtWoH5rYzYKpv3_Qpp_CyY3ZqDFQ_JghI70_dDFR2THNY_J_Q1DwSfkx_Et7InFeFSX0iX679_SRdeu6JGbVtffnaVfL-FS2-b3z189PYWGb3QoL7ieAiSko21iR1tP59DcTwe5CDbVAFPpx0Et2a3x1fMVPap785ScLY6_zE_i8Q8LsRE5W-On0z6VgFxZ7kxqstSkXnvOneUScFQG7NHpRFqtbZV4KbWR1qbWWy00K03Cn5G9pm3cC0LhSZEbncGUaz1yUpmm3ANX8UJYk1cyIu-mkVbXg5GGAgKCAVIhQAoDpEKAIvIBQ3G3HVpghxVtd6HGRFDQcVYCX-TWOeD4heaurNKKawGMsspdRN5MgVRwy-AYV41rb3rFsM4DxI1lEXk-BPbuVFNiRKTYCvlWX7ZbIEuDLfeYlfv_vedLcg_HABVurHhF9tbdjXsNkGetD0KpAJZLIQ9Cuv8B99wH5g |
link.rule.ids | 230,315,733,786,790,870,891,2115,24346,27955,27956,53825,53827 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nc5QwGM7UelAP9dviZ5zx4oFdCAHCUbfd2dZux0OrvWXIV8vYhQ6wM7UHx7_h3_OX-CZAZ7fjRa8ESOB9Q54neXiC0DtKqQgzQyACWvvUyMAXgdF-lAuVSACozNk1zQ-T2THdP4lPNlA8_AvjRPtSFKPyfDEqizOnrbxYyPGgExt_nk9Si4oDOr6FbkN_JfEKSe-mVgCDMNItSgIFy8ZGFJX1xCRWx0USu4UNJHJGUqvgWhmPnG3_37DmTcnkyhg0vY--DK3vpCffRstWjOTVDWPHf368B2irR6X4Q1f8EG3o8hG6t-JV-Bj92L2EJtl5fitcxXNr7X-Jp3W1wDt6OFxcaYW_nkE1Vfn7568G70PBd9zNXOgGA9rEvSNjjSuDJ1DcDDc5dQ7YgIDxXifErFu7qn2Od4pGPkHH092jyczvN2_wJU1Ia__KNiEDUEwSLUMZhzI0wkSRVhEDiBYDMdUiYEoIlQeGMSGZUqEySlBBMhlET9FmWZV6G2H4CCVSxDCaK2PpLgvDyAANMpQqmeTMQ--HEPKLzqODA7exkecu8txGnrvIe-ijjfH1edZd2x2o6lPeB4FDw0kGVDRSWtMkS0WkszzMI0GBrOaJ9tDbIUM49Eb7jvNSV8uGEzuFBJyQxB561mXMdVVDxnkoXcultbasl0CGOMfvPiOe__eVb9Cd2dH8gB_sHX56ge7a92GFdCR9iTbbeqlfAbJqxWvXj_4A0RgoBA |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3LbpwwFLXaVKraRfpu6NOVuumCAYwBs2xnMkrSTpRFo0bdIPxKUDMwAkZKs6j6G_29fkmvDURM1FW2GLDxvcbnmMMxQu8ppTxINYEIKOVSLXyX-1q5Yc5lLACgMmvXtDiM947pwUl0Mtrqy4r2BS8m5flyUhZnVlu5Wgpv0Il5R4tpYlCxT72V1N5tdAfGLElGRL1bXgEcwkj3YRJoWOppXlTGF5MYLReJzTY2kMwpSYyKazQnWev-_-HN67LJ0Tw0f4C-D0_QyU9-TNYtn4jLa-aON3rEh2i7R6f4Y3fKI3RLlY_R_ZFn4RP0a_cCmmXW-42AFS-Mxf8FntfVEs_UcLi4VBJ_O4OqqvLv7z8NPoCCn7hbwVANBtSJe2fGGlcaT6G4GW5yap2wAQnj_U6QWbfm6_Y5nhWNeIqO57tfp3tuv4mDK2hMWvN3tg4YgGMSKxGIKBCB5joMlQwZQLUICKriPpOcy9zXjHHBpAyklpxykgo_fIa2yqpUOwjDyygWPIJZXWpDe1kQhBrokKZUijhnDvowhDFbdV4dGXAcE_3MRj8z0c9s9B30ycT56jzjsm0PVPVp1gcig4aTFChpKJWicZrwUKV5kIecAmnNY-Wgd0OWZDAqTR_nparWTUbMUhJwQxI56HmXNVdVDVnnoGQjnzbaslkCWWKdv_useHHjK9-iu0ezefZl__DzS3TPdIfR05HkFdpq67V6DQCr5W_sUPoH5-cqhA |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Extracellular+Matrix+From+Decellularized+Wharton%27s+Jelly+Improves+the+Behavior+of+Cells+From+Degenerated+Intervertebral+Disc&rft.jtitle=Frontiers+in+bioengineering+and+biotechnology&rft.au=Penolazzi%2C+Letizia&rft.au=Pozzobon%2C+Michela&rft.au=Bergamin%2C+Leticia+Scussel&rft.au=D%27Agostino%2C+Stefania&rft.date=2020-03-27&rft.issn=2296-4185&rft.eissn=2296-4185&rft.volume=8&rft.spage=262&rft.epage=262&rft_id=info:doi/10.3389%2Ffbioe.2020.00262&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2296-4185&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2296-4185&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2296-4185&client=summon |