A New Tool for Complement Research: In vitro Reconstituted Human Classical Complement Pathway

• Published in: Frontiers in immunology Vol. 9; p. 2770
• Main Authors: Mutti, Michele, Ramoni, Katharina, Nagy, Gábor, Nagy, Eszter, Szijártó, Valéria
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 04.12.2018
• Subjects: alternative pathway; Animals; Antibodies, Monoclonal - immunology; bactericidal activity; Biological Assay - methods; Blood Bactericidal Activity - immunology; C1-INH; classical pathway; Complement Activation - immunology; Complement Membrane Attack Complex - immunology; Complement Pathway, Alternative - immunology; Complement Pathway, Classical - immunology; Complement System Proteins - immunology; E. coli ST131; Escherichia coli - immunology; Humans; Immunology; monoclonal Ab; O Antigens - immunology; Rabbits; alternative pathway; classical pathway; complement system; C1-INH; E. coli ST131; bactericidal activity; MG1655; monoclonal Ab

The complement, as part of the innate immune system, represents the first line of defense against Gram-negative bacteria invading the bloodstream. The complement system is a zymogen cascade that ultimately assemble into the so-called membrane attack complex (MAC), which lyses Gram-negative bacteria...