Increased symmetrical dimethylarginine in ischemic acute kidney injury as a causative factor of renal L-arginine deficiency

• Published in: Translational research : the journal of laboratory and clinical medicine Vol. 162; no. 2; pp. 67 - 76
• Main Authors: Betz, Boris, Möller-Ehrlich, Kerstin, Kress, Tobias, Kniepert, Joachim, Schwedhelm, Edzard, Böger, Rainer H., Wanner, Christoph, Sauvant, Christoph, Schneider, Reinhard
• Format: Journal Article
• Language: English
• Published: United States Mosby, Inc 01.08.2013
• Subjects: Acute Kidney Injury - metabolism; Acute Kidney Injury - physiopathology; Amidohydrolases - metabolism; Animals; Arginine - analogs & derivatives; Arginine - blood; Arginine - deficiency; Arginine - metabolism; Blotting, Western; Chromatography, High Pressure Liquid; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Female; Internal Medicine; Intracellular Signaling Peptides and Proteins - metabolism; Kidney - metabolism; Kidney Function Tests; Protein-Arginine N-Methyltransferases - metabolism; Rats; Rats, Sprague-Dawley; Real-Time Polymerase Chain Reaction; Reperfusion Injury - metabolism; Reperfusion Injury - physiopathology; Tandem Mass Spectrometry; eNOS; GFR; NOS; SDMA; PAH; CAT; DDAH; ADMA; iNOS; RPF; PRMT; PCR; para-aminohippurate; glomerular filtration rate; asymmetrical dimethylarginine; cationic amino acid transporter; symmetrical dimethylarginine; inducible nitric oxide synthase; protein arginine methyltransferase; renal plasma flow; dimethylarginine dimethylaminohydrolase; endothelial nitric oxide synthase; nitric oxide synthase; polymerase chain reaction

Availability of L-arginine, the exclusive substrate for nitric oxide synthases, plays an important role in kidney ischemia/reperfusion injury. The endogenous L-arginine derivatives asymmetrical dimethylarginine (ADMA) and symmetrical dimethylarginine (SDMA) block cellular L-arginine uptake competiti...