Use of the Roche LightCycler ® 480 system in a routine laboratory setting for molecular diagnosis of opportunistic viral infections: Evaluation on whole blood specimens and proficiency panels

• Published in: Journal of virological methods Vol. 159; no. 2; pp. 291 - 294
• Main Authors: Deback, Claire, Géli, Julienne, Aït-Arkoub, Zaïna, Angleraud, Francis, Gautheret-Dejean, Agnès, Agut, Henri, Boutolleau, David
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.08.2009; Elsevier
• Subjects: Biological and medical sciences; BK Virus - isolation & purification; Blood - virology; Cytomegalovirus - isolation & purification; Fundamental and applied biological sciences. Psychology; Herpesvirus 4, Human - isolation & purification; Herpesvirus 6, Human - isolation & purification; Humans; LightCycler ® 480 system; Microbiology; Molecular Diagnostic Techniques - methods; Opportunistic viral infections; Polymerase Chain Reaction - methods; Techniques used in virology; Viral load; Viral molecular diagnosis; Virology; Virus Diseases - diagnosis; Viruses - isolation & purification; Whole blood specimen; Whole blood specimen; Viral molecular diagnosis; Opportunistic viral infections; Viral load; LightCycler ® 480 system; Opportunistic infection; Microbiology; LightCycler; Method; Bacteremia; Virology; Infection; Whole blood; Viral disease; Bacteriosis; Diagnosis; 480 system

The Roche LightCycler ® 480 (LC480) system was evaluated for quantitative molecular diagnosis of opportunistic viral infections caused by human cytomegalovirus (HCMV), Epstein–Barr virus (EBV), human herpesvirus 6 (HHV-6) and BK virus (BKV), in comparison with “in-house” real-time PCR assays. A tota...