Automated derivatization and fluorimetric determination of biogenic amines in milk by zone fluidics coupled to liquid chromatography

• Published in: Journal of Chromatography A Vol. 1356; pp. 272 - 276
• Main Authors: Notou, Maria, Zotou, Anastasia, Tzanavaras, Paraskevas D., Themelis, Demetrius G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22.08.2014; Elsevier
• Subjects: Animals; Autoanalysis; Biogenic amines; Biogenic Amines - analysis; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Fluorometry; Food industries; Fundamental and applied biological sciences. Psychology; Liquid chromatography; Milk; Milk - chemistry; Milk and cheese industries. Ice creams; Naphthalene-2,3-dicarboxaldehyde; Naphthalenes - chemistry; Reference Standards; Sequential injection analysis; Naphthalene-2,3-dicarboxaldehyde; Sequential injection analysis; Liquid chromatography; Biogenic amines; Milk; Validation; Automation; Fluorescence detector; Chemical analysis; HPLC chromatography; Derivatization; Trademark; Fluorescent labelling; Biogenic amine; Simultaneous measurement; Sequential injection; Quantitative analysis

•A novel zone-fluidics (ZF)–HPLC method is described for the determination of biogenic amines.•On-line derivatization of the amines with naphthalene-2,3-dicarboxaldehyde (NDA) is performed using ZF.•Using ZF the consumption of the expensive NDA is minimized to a few micro-liters per run. A novel zon...