Gentamicin-impregnated chitosan/nanohydroxyapatite/ethyl cellulose microspheres granules for chronic osteomyelitis therapy

• Published in: Journal of biomedical materials research. Part A Vol. 93A; no. 3; pp. 1020 - 1031
• Main Authors: Shi, Pujiang, Zuo, Yi, Li, Xiaowei, Zou, Qin, Liu, Haohuai, Zhang, Li, Li, Yubao, Morsi, Yos S
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2010; Wiley-Blackwell
• Subjects: Animals; Bacterial arthritis and osteitis; Bacterial diseases; Biological and medical sciences; Cell Death - drug effects; Cell Survival - drug effects; Cellulose - analogs & derivatives; Cellulose - pharmacology; Chitosan - pharmacology; Chronic Disease; Durapatite - pharmacology; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; gentamicin release; Gentamicins - pharmacology; Gentamicins - therapeutic use; granule; Human bacterial diseases; Humans; Implants, Experimental; Infectious diseases; Medical sciences; microsphere; Microspheres; Nanostructures - chemistry; Nanostructures - ultrastructure; Osteoblasts - cytology; Osteoblasts - drug effects; Osteoblasts - metabolism; osteomyelitis; Osteomyelitis - drug therapy; Osteomyelitis - pathology; Rabbits; Radiography; Rats; Rats, Wistar; Staphylococcus aureus; Staphylococcus aureus - drug effects; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Tibia - diagnostic imaging; Tibia - drug effects; Tibia - microbiology; Tibia - pathology; granule; Microsphere; Gentamicin; Diseases of the osteoarticular system; Cellulose(ethyl); gentamicin release; osteomyelitis; Chronic; Treatment; Osteitis; Cellulose derivatives; Oside polymer; Biomaterial; Chitosan; Cellulose ether; Biomedical engineering
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32598

In this article gentamicin (GM) impregnated microspheres were used to extend the drug release time for the treatment of chronic osteomyelitis. The granules were prepared in solution and consisted of nanohydroxyapatite (nHA), chitosan (CS) and GM loaded ethyl cellulose (EC) microspheres. A rabbit model with chronic osteomyelitis was made by using staphylococcus aureus and morrhuate sodium and special inspection methods were used to test the curative effects of the granules, such as microbiological investigations, tissue, and X‐ray observations. The granules were provided with excellent drug release properties, 49 days in vitro and 45 days in vivo, moreover, they showed almost no cytotoxic for fibroblast and osteoblast. The findings indicated that the GM‐impregnated CS/nHA/EC microspheres granules showed outstanding curative effect. Generally, it can be concluded that the granules containing GM impregnated microspheres may be used effectively in the treatment of the chronic osteomyelitis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010