A novel UPLC-MS/MS method for sensitive quantitation of boldine in plasma, a potential anti-inflammatory agent: application to a pharmacokinetic study in rats

• Published in: Biomedical chromatography Vol. 29; no. 3; pp. 459 - 464
• Main Authors: Zeng, Rong-Jie, Li, Yu, Chen, Jian-Zhong, Chou, Gui-Xin, Gao, Yu, Shao, Jing-Wei, Jia, Lee, Wu, Sheng-Dong, Wu, Shui-Sheng
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.03.2015
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - administration & dosage; Anti-Inflammatory Agents, Non-Steroidal - analysis; Anti-Inflammatory Agents, Non-Steroidal - pharmacokinetics; Aporphines - administration & dosage; Aporphines - blood; Aporphines - pharmacokinetics; boldine; Calibration; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Drug Stability; Injections, Intravenous; Limit of Detection; liquid-liquid extraction; Male; pharmacokinetics; quantitation; Rats, Sprague-Dawley; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; ultra-high performance liquid chromatography-tandem mass spectrometry; pharmacokinetics; ultra-high performance liquid chromatography-tandem mass spectrometry; boldine; liquid-liquid extraction; quantitation

Boldine is a potential anti‐inflammatory agent found in several different plants. Published bioanalytical methods using HPLC with ultraviolet and fluorescent detection lacked enough sensitivity and required tedious sample preparation procedures. Herein, we describe the development of a novel ultra‐h...