In vitro response of monocyte-derived macrophages to a decellularized pericardial biomaterial

Decellularized tissue‐derived heart valves are an example of biomaterials derived from natural scaffolds. These types of implants are increasing in popularity although their in vivo performance is still only poorly understood and has, at times, been catastrophic. It is apparent that better understan...

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Published inJournal of biomedical materials research. Part A Vol. 93A; no. 1; pp. 280 - 288
Main Authors Ariganello, Marianne B., Labow, Rosalind S., Lee, J. Michael
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2010
Wiley-Blackwell
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Summary:Decellularized tissue‐derived heart valves are an example of biomaterials derived from natural scaffolds. These types of implants are increasing in popularity although their in vivo performance is still only poorly understood and has, at times, been catastrophic. It is apparent that better understanding is required before these biomaterials can be used safely. In this study, the human monocyte‐derived macrophage (MDM) response to decellularized bovine pericardium (DBP) was used as a model to predict the biological performance of these materials on implantation. Human monocytes differentiated on tissue culture polystyrene (TCPS) for 14 days were trypsinized and reseeded onto DBP, TCPS, and polydimethylsiloxane (PDMS) for 48 h. The MDMs on DBP contained less intracellular and extracellular esterase activity compared with MDMs on TCPS and PDMS, as well as less acid phosphatase activity than on TCPS. As well, morphologically, MDMs on DBP were less spread, less multinucleated and did not display many lamellipodia. Taken together, these data represent the first evidence of the MDM response to intact, native extracellular matrix, demonstrating that these cells reacted with an altered, possibly reduced foreign body response on this natural scaffold compared with the two control surfaces. This in vitro MDM cell model may provide a novel method for predicting and elucidating the biological performance of tissue‐derived biomaterials, thereby directing a more rational design of biomaterials for tissue regeneration purposes. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010
Bibliography:Canadian Federation of University Women (CFUW)
CIHR Strategic Training Fellowship in Cell Signaling in Mucosal Inflammation and Pain - No. STP53877
ArticleID:JBM32554
Institute for Musculoskeletal Health and Arthritis (IMHA)
istex:B30310C1F5E60C8F16B75EA379B9AF2055AC65F5
Natural Science and Engineering Research Council (NSERC)
ark:/67375/WNG-9HQZ6FZH-2
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32554