Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs

The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however,...

Full description

Saved in:
Bibliographic Details
Published inMetabolites Vol. 10; no. 8; p. 322
Main Authors Taniguchi, Masaaki, Arakawa, Aisaku, Nishio, Motohide, Okamura, Toshihiro, Ohnishi, Chika, Kadowaki, Kouen, Kohira, Kimiko, Homma, Fumika, Matsumoto, Kazunori, Ishii, Kazuo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 07.08.2020
MDPI
Subjects
Amino acids
biomarker
Capillary electrophoresis
CE-TOFMS
Gene expression
Hogs
intramuscular fat
Livestock
Mass spectroscopy
Meat quality
Metabolites
Metabolomics
porcine
Pork
Principal components analysis
Online AccessGet full text

Cover

Abstract The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however, no studies have investigated their use during the fattening period of pigs. This study examined the metabolite profiles in the plasma of two groups of pigs derived from the same Duroc genetic line and fed the same diet. Five plasma samples were collected from each individual the day before slaughter. Capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS) was used to analyze the purified plasma from each sample. Principle component analysis (PCA) and partial least squares (PLS) were used to find the semi-quantitative values of the compounds. The results indicate that branched-chain amino acids are significantly associated with high IMF content, while amino acids are associated with low IMF content. These differences were validated using the quantification analyses by high-performance liquid chromatograph, which supported our results. These results suggest that the concentration of branched-chain amino acids in plasma could be an indicative biomarker for the IMF content in the loin eye area.
AbstractList The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are currently impractical without a labor-intensive process. Metabolomic profiling could be used as an IMF indicator to avoid this process; however, no studies have investigated their use during the fattening period of pigs. This study examined the metabolite profiles in the plasma of two groups of pigs derived from the same Duroc genetic line and fed the same diet. Five plasma samples were collected from each individual the day before slaughter. Capillary electrophoresis-time of flight mass spectrometry (CE-TOFMS) was used to analyze the purified plasma from each sample. Principle component analysis (PCA) and partial least squares (PLS) were used to find the semi-quantitative values of the compounds. The results indicate that branched-chain amino acids are significantly associated with high IMF content, while amino acids are associated with low IMF content. These differences were validated using the quantification analyses by high-performance liquid chromatograph, which supported our results. These results suggest that the concentration of branched-chain amino acids in plasma could be an indicative biomarker for the IMF content in the loin eye area.
Author Kohira, Kimiko
Nishio, Motohide
Ohnishi, Chika
Matsumoto, Kazunori
Arakawa, Aisaku
Homma, Fumika
Taniguchi, Masaaki
Okamura, Toshihiro
Kadowaki, Kouen
Ishii, Kazuo
AuthorAffiliation 1 Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Tsukuba Ibaraki 305-0901, Japan; aisaku@affrc.go.jp (A.A.); mtnishio@affrc.go.jp (M.N.); okamut@affrc.go.jp (T.O.); kazishi@affrc.go.jp (K.I.)
2 Miyazaki Station, National Livestock Breeding Center, Kobayashi Miyazaki 886-0004, Japan; c0ohnisi@nlbc.go.jp
4 National Livestock Breeding Center, Nishigo Fukushima 961-8511, Japan; k0kohira@nlbc.go.jp (K.K.); f0honma@nlbc.go.jp (F.H.); k0matsumoto@nlbc.go.jp (K.M.)
3 Ibaraki Station, National Livestock Breeding Center, Chikusei Ibaraki 308-0112, Japan; k0kadowk@nlbc.go.jp
AuthorAffiliation_xml – name: 2 Miyazaki Station, National Livestock Breeding Center, Kobayashi Miyazaki 886-0004, Japan; c0ohnisi@nlbc.go.jp
– name: 3 Ibaraki Station, National Livestock Breeding Center, Chikusei Ibaraki 308-0112, Japan; k0kadowk@nlbc.go.jp
– name: 1 Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization, Tsukuba Ibaraki 305-0901, Japan; aisaku@affrc.go.jp (A.A.); mtnishio@affrc.go.jp (M.N.); okamut@affrc.go.jp (T.O.); kazishi@affrc.go.jp (K.I.)
– name: 4 National Livestock Breeding Center, Nishigo Fukushima 961-8511, Japan; k0kohira@nlbc.go.jp (K.K.); f0honma@nlbc.go.jp (F.H.); k0matsumoto@nlbc.go.jp (K.M.)
Author_xml – sequence: 1
  givenname: Masaaki
  surname: Taniguchi
  fullname: Taniguchi, Masaaki
– sequence: 2
  givenname: Aisaku
  surname: Arakawa
  fullname: Arakawa, Aisaku
– sequence: 3
  givenname: Motohide
  surname: Nishio
  fullname: Nishio, Motohide
– sequence: 4
  givenname: Toshihiro
  surname: Okamura
  fullname: Okamura, Toshihiro
– sequence: 5
  givenname: Chika
  surname: Ohnishi
  fullname: Ohnishi, Chika
– sequence: 6
  givenname: Kouen
  surname: Kadowaki
  fullname: Kadowaki, Kouen
– sequence: 7
  givenname: Kimiko
  surname: Kohira
  fullname: Kohira, Kimiko
– sequence: 8
  givenname: Fumika
  surname: Homma
  fullname: Homma, Fumika
– sequence: 9
  givenname: Kazunori
  surname: Matsumoto
  fullname: Matsumoto, Kazunori
– sequence: 10
  givenname: Kazuo
  surname: Ishii
  fullname: Ishii, Kazuo
BookMark eNpdkktv1DAQgCNUREvplbMlLlxS_IoTX5CqpUtX2qqVKGfLTsapV4ld7IRq_z1Ot0Is44PHnk-f_Jj3xYkPHoriI8GXjEn8ZYRJm0AwbjCj9E1xRilpSiIbefJPflpcpLTDOQSuakzeFaeM1g2vBT0r9t-ctRDBT04P6PZFOITRtQndx2DdAAltuqVsHXTI7NHquny4W9_-QAamZwCPblz_iLTv0DY8o42foh7n1M6DjmitJ3Q1htlPyPllNYF3vkf3rk8firdWDwkuXufz4uf6-mF1U27vvm9WV9uy5YJMpeWNpZR2xhrgrBNgBdMMqBCmoRUAIbSxhteWcVZRTuocbUMoNV1rrenYebE5eLugd-opulHHvQraqZeNEHul4-TaAZRutcGiyhIiuQVqpBR5GGIrhmWls-vrwfU0mxG6FpbbDkfS44p3j6oPv1XNBee0yoLPr4IYfs2QJjW61MIwaA9hTopyxvMnCSkz-uk_dBfm6PNTLRQVEktMMnV5oNoYUopg_x6GYLU0iTpuEvYHEWewyQ
CitedBy_id crossref_primary_10_1016_j_meatsci_2024_109574
crossref_primary_10_3390_foods10102388
crossref_primary_10_3390_metabo12030242
crossref_primary_10_1021_acs_jafc_3c02669
crossref_primary_10_1071_AN21512
crossref_primary_10_1016_j_cofs_2023_101088
crossref_primary_10_1038_s42003_023_05266_3
Cites_doi 10.1016/j.meatsci.2017.11.024
10.3390/nu9010017
10.1111/asj.12520
10.1021/jf101471d
10.1371/journal.pone.0128350
10.1039/c0mb00141d
10.3390/metabo10050188
10.2527/af.2016-0011
10.2527/jas.2006-752
10.1186/s12711-016-0217-x
10.2527/2005.8392058x
10.2527/2005.832316x
10.1111/j.1740-0929.2004.00195.x
10.1039/B714176A
10.1016/j.meatsci.2014.07.018
10.2527/jas.2011-4404
10.1093/jn/134.9.2191
10.1111/j.1740-0929.2010.00844.x
10.1186/s40104-018-0268-7
10.1111/asj.12622
10.1093/jn/132.12.3744
10.1017/S1751731109991091
10.5713/ajas.17.0218
10.1016/j.livsci.2008.10.006
10.1186/s40104-018-0276-7
10.1186/s40104-018-0306-5
10.1002/cem.2883
10.2527/jas.2008-1336
10.1016/S0309-1740(02)00207-3
10.1017/S1751731118002082
ContentType Journal Article
Copyright 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2020 by the authors. 2020
Copyright_xml – notice: 2020. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2020 by the authors. 2020
DBID AAYXX
CITATION
7QR
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.3390/metabo10080322
DatabaseName CrossRef
Chemoreception Abstracts
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
Biological Science Database
Biotechnology and BioEngineering Abstracts
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle CrossRef
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest Biological Science Collection
ProQuest Central Essentials
ProQuest One Academic Eastern Edition
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
Biological Science Database
ProQuest SciTech Collection
ProQuest Central China
Biotechnology and BioEngineering Abstracts
ProQuest Central
ProQuest One Academic UKI Edition
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
Chemoreception Abstracts
Engineering Research Database
ProQuest One Academic
MEDLINE - Academic
DatabaseTitleList
Publicly Available Content Database

CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
EISSN 2218-1989
ExternalDocumentID oai_doaj_org_article_acab065417194fe2b996969b1f53095a
10_3390_metabo10080322
GroupedDBID 53G
5VS
8FE
8FH
AADQD
AAFWJ
AAYXX
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
BBNVY
BENPR
BHPHI
CCPQU
CITATION
DIK
GROUPED_DOAJ
HCIFZ
HYE
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
PGMZT
PIMPY
PROAC
RIG
RPM
7QR
8FD
ABUWG
AZQEC
DWQXO
FR3
GNUQQ
P64
PQEST
PQQKQ
PQUKI
PRINS
7X8
5PM
ID FETCH-LOGICAL-c461t-f48f222dbfbe43d6ef63a3e266b825ee1128fb47f34352417777c8122bdcffbd3
IEDL.DBID RPM
ISSN 2218-1989
IngestDate Tue Oct 22 15:08:43 EDT 2024
Tue Sep 17 21:23:28 EDT 2024
Fri Aug 16 01:25:46 EDT 2024
Thu Oct 10 17:31:42 EDT 2024
Thu Aug 22 14:41:24 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 8
Language English
License Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c461t-f48f222dbfbe43d6ef63a3e266b825ee1128fb47f34352417777c8122bdcffbd3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0002-4691-4436
0000-0001-8194-3577
0000-0002-7438-8269
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7464425/
PMID 32784762
PQID 2432690901
PQPubID 2032362
ParticipantIDs doaj_primary_oai_doaj_org_article_acab065417194fe2b996969b1f53095a
pubmedcentral_primary_oai_pubmedcentral_nih_gov_7464425
proquest_miscellaneous_2434057699
proquest_journals_2432690901
crossref_primary_10_3390_metabo10080322
PublicationCentury 2000
PublicationDate 20200807
PublicationDateYYYYMMDD 2020-08-07
PublicationDate_xml – month: 8
  year: 2020
  text: 20200807
  day: 7
PublicationDecade 2020
PublicationPlace Basel
PublicationPlace_xml – name: Basel
PublicationTitle Metabolites
PublicationYear 2020
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Hovenier (ref_7) 1993; 14
Muroya (ref_15) 2014; 98
Suravajhala (ref_18) 2016; 48
Newcom (ref_9) 2005; 83
ref_35
Schwab (ref_3) 2010; 88
ref_12
Ward (ref_28) 2015; 90
Smriga (ref_24) 2002; 132
ref_16
Davoli (ref_6) 2019; 13
Ooga (ref_30) 2011; 7
Hocquette (ref_1) 2010; 4
Sakuma (ref_32) 2017; 88
Won (ref_5) 2018; 31
Suzuki (ref_10) 2005; 83
(ref_4) 2003; 64
Kruk (ref_27) 2018; 137
Solanes (ref_11) 2009; 123
Luo (ref_19) 2018; 9
Katsumata (ref_14) 2011; 82
ref_23
Zhang (ref_20) 2016; 87
ref_21
McGillivray (ref_13) 2004; 134
Fontanesi (ref_17) 2016; 6
Srinongkote (ref_25) 2004; 75
Sugimoto (ref_31) 2010; 58
Yamamoto (ref_34) 2017; 33
Okumura (ref_33) 2007; 85
ref_2
ref_8
Harris (ref_26) 2018; 9
Wang (ref_22) 2018; 9
Ohashi (ref_29) 2008; 4
References_xml – volume: 137
  start-page: 139
  year: 2018
  ident: ref_27
  article-title: Vitamin A and Marbling Attributes: Intramuscular Fat Hyperplasia Effects in Cattle
  publication-title: Meat Sci.
  doi: 10.1016/j.meatsci.2017.11.024
  contributor:
    fullname: Kruk
– ident: ref_21
  doi: 10.3390/nu9010017
– volume: 87
  start-page: 911
  year: 2016
  ident: ref_20
  article-title: Effects of dietary leucine supplementation in low crude protein diets on performance, nitrogen balance, whole-body protein turnover, carcass characteristics and meat quality of finishing pigs
  publication-title: Anim. Sci. J.
  doi: 10.1111/asj.12520
  contributor:
    fullname: Zhang
– volume: 58
  start-page: 8418
  year: 2010
  ident: ref_31
  article-title: Metabolomic profiles and sensory attributes of edamame under various storage duration and temperature conditions
  publication-title: J. Agric. Food Chem.
  doi: 10.1021/jf101471d
  contributor:
    fullname: Sugimoto
– ident: ref_12
  doi: 10.1371/journal.pone.0128350
– volume: 7
  start-page: 1217
  year: 2011
  ident: ref_30
  article-title: Metabolomic anatomy of an animal model revealing homeostatic imbalances in dyslipidaemia
  publication-title: Mol. Biosyst.
  doi: 10.1039/c0mb00141d
  contributor:
    fullname: Ooga
– ident: ref_16
  doi: 10.3390/metabo10050188
– volume: 6
  start-page: 73
  year: 2016
  ident: ref_17
  article-title: Metabolomics and livestock genomics: Insights into a phenotyping frontier andits applications in animal breeding
  publication-title: Anim. Front.
  doi: 10.2527/af.2016-0011
  contributor:
    fullname: Fontanesi
– volume: 85
  start-page: 1902
  year: 2007
  ident: ref_33
  article-title: Intramuscular fat deposition in principal muscles from twenty-four to thirty months of age using identical twins of Japanese Black steers
  publication-title: J. Anim. Sci.
  doi: 10.2527/jas.2006-752
  contributor:
    fullname: Okumura
– volume: 48
  start-page: 38
  year: 2016
  ident: ref_18
  article-title: Multi-omic data integration and analysis using systems genomics approaches: Methods and applications in animal production, health and welfare
  publication-title: Genet. Sel. Evol.
  doi: 10.1186/s12711-016-0217-x
  contributor:
    fullname: Suravajhala
– volume: 83
  start-page: 2058
  year: 2005
  ident: ref_10
  article-title: Genetic parameter estimates of meat quality traits in Duroc pigs selected for average daily gain, longissimus muscle area, backfat thickness, and intramuscular fat content
  publication-title: J. Anim. Sci.
  doi: 10.2527/2005.8392058x
  contributor:
    fullname: Suzuki
– volume: 83
  start-page: 316
  year: 2005
  ident: ref_9
  article-title: Genetic and phenotypic relationships between individual subcutaneous backfat layers and percentage of longissimus intramuscular fat in Duroc swine
  publication-title: J. Anim. Sci.
  doi: 10.2527/2005.832316x
  contributor:
    fullname: Newcom
– volume: 75
  start-page: 339
  year: 2004
  ident: ref_25
  article-title: Diet supplied with L-lysine and L-arginine during chronic stress of high stock density normalizes growth of broilers
  publication-title: Anim. Sci. J.
  doi: 10.1111/j.1740-0929.2004.00195.x
  contributor:
    fullname: Srinongkote
– ident: ref_35
– ident: ref_23
– volume: 4
  start-page: 135
  year: 2008
  ident: ref_29
  article-title: Depiction of metabolome changes in histidine-starved Escherichia coli by CE-TOFMS
  publication-title: Mol. Biosyst.
  doi: 10.1039/B714176A
  contributor:
    fullname: Ohashi
– volume: 98
  start-page: 726
  year: 2014
  ident: ref_15
  article-title: CE-TOF MS-based metabolomic profiling revealed characteristic metabolic pathways in postmortem porcine fast and slow type muscles
  publication-title: Meat Sci.
  doi: 10.1016/j.meatsci.2014.07.018
  contributor:
    fullname: Muroya
– volume: 90
  start-page: 2476
  year: 2015
  ident: ref_28
  article-title: The impact of vitamin A restriction and ADH1C genotype on marbling in feedlot steers
  publication-title: J. Anim. Sci.
  doi: 10.2527/jas.2011-4404
  contributor:
    fullname: Ward
– volume: 134
  start-page: 2191
  year: 2004
  ident: ref_13
  article-title: Restriction of dietary energy and protein induces molecular changes in young porcine skeletal muscles
  publication-title: J. Nutr.
  doi: 10.1093/jn/134.9.2191
  contributor:
    fullname: McGillivray
– volume: 82
  start-page: 17
  year: 2011
  ident: ref_14
  article-title: Promotion of intramuscular fat accumulation in porcine muscle by nutritional regulation
  publication-title: Anim. Sci. J.
  doi: 10.1111/j.1740-0929.2010.00844.x
  contributor:
    fullname: Katsumata
– volume: 9
  start-page: 55
  year: 2018
  ident: ref_26
  article-title: Vitamin A administration at birth promotes calf growth and intramuscular fat development in Angus beef cattle
  publication-title: J. Anim. Sci. Biotechnol.
  doi: 10.1186/s40104-018-0268-7
  contributor:
    fullname: Harris
– ident: ref_8
– volume: 88
  start-page: 203
  year: 2017
  ident: ref_32
  article-title: Estimates of genetic parameters for chemical traits of meat quality in Japanese black cattle
  publication-title: Anim. Sci. J.
  doi: 10.1111/asj.12622
  contributor:
    fullname: Sakuma
– ident: ref_2
– volume: 132
  start-page: 3744
  year: 2002
  ident: ref_24
  article-title: DietaryL-lysine deficiency increases stress-induced anxiety and fecal excretion in rats
  publication-title: J. Nutr.
  doi: 10.1093/jn/132.12.3744
  contributor:
    fullname: Smriga
– volume: 4
  start-page: 303
  year: 2010
  ident: ref_1
  article-title: Intramuscular fat content in meat-producing animals: Development, genetic and nutritional control, and identification of putative markers
  publication-title: Animal
  doi: 10.1017/S1751731109991091
  contributor:
    fullname: Hocquette
– volume: 31
  start-page: 157
  year: 2018
  ident: ref_5
  article-title: Identification of genes related to intramuscular fat content of pigs using genome-wide association study
  publication-title: Asian Aust. J. Anim. Sci.
  doi: 10.5713/ajas.17.0218
  contributor:
    fullname: Won
– volume: 123
  start-page: 63
  year: 2009
  ident: ref_11
  article-title: Genetic correlations and expected response for intramuscular fat content in a Duroc pig line
  publication-title: Livest. Prod. Sci.
  doi: 10.1016/j.livsci.2008.10.006
  contributor:
    fullname: Solanes
– volume: 9
  start-page: 60
  year: 2018
  ident: ref_22
  article-title: Advances in low-protein diets for swine
  publication-title: J. Anim. Sci. Biotech.
  doi: 10.1186/s40104-018-0276-7
  contributor:
    fullname: Wang
– volume: 9
  start-page: 88
  year: 2018
  ident: ref_19
  article-title: Surplus dietary isoleucine intake enhanced monounsaturated fatty acid synthesis and fat accumulation in skeletal muscle of finishing pigs
  publication-title: J. Anim. Sci. Biotech.
  doi: 10.1186/s40104-018-0306-5
  contributor:
    fullname: Luo
– volume: 33
  start-page: e2883
  year: 2017
  ident: ref_34
  article-title: PLS-ROG: Partial least squares with rank order of groups
  publication-title: J. Chemom.
  doi: 10.1002/cem.2883
  contributor:
    fullname: Yamamoto
– volume: 88
  start-page: 69
  year: 2010
  ident: ref_3
  article-title: Results from six generations of selection for intramuscular fat in Duroc swine using real-time ultrasound. Ⅱ. Genetic parameters and trends
  publication-title: J. Anim. Sci.
  doi: 10.2527/jas.2008-1336
  contributor:
    fullname: Schwab
– volume: 14
  start-page: 17N
  year: 1993
  ident: ref_7
  article-title: Breeding for pig meat quality in halothane negative populations—A review
  publication-title: Pig News Inf.
  contributor:
    fullname: Hovenier
– volume: 64
  start-page: 405
  year: 2003
  ident: ref_4
  article-title: Genetic parameters for meat and fat quality and carcass composition traits in Iberian pigs
  publication-title: Meat Sci.
  doi: 10.1016/S0309-1740(02)00207-3
– volume: 13
  start-page: 924
  year: 2019
  ident: ref_6
  article-title: Genetic parameters of backfat fatty acids and carcass traits in Large White pigs
  publication-title: Animal
  doi: 10.1017/S1751731118002082
  contributor:
    fullname: Davoli
SSID ssj0000605701
Score 2.242215
Snippet The amount of intramuscular fat (IMF) present in the loin eye area is one of the most important characteristics of high-quality pork. IMF measurements are...
SourceID doaj
pubmedcentral
proquest
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
StartPage 322
SubjectTerms Amino acids
biomarker
Capillary electrophoresis
CE-TOFMS
Gene expression
Hogs
intramuscular fat
Livestock
Mass spectroscopy
Meat quality
Metabolites
Metabolomics
porcine
Pork
Principal components analysis
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrZ3BT9swFMatiROXCegmMhh6SNN2ikhsJ06OBVrBtG5IA4lbZDf2qNSmaE2F-t_znh1Qc-JCb21SyfWz-35f-_w9xr6ZxHGpuYozWaNAcShQSq5MrESBGbYQqfb2xZPf-dWd_Hmf3W-1-qKasGAPHCbuTE-1oQOQqUK57Sw3Jfm5lCZ1mUA8CGiUlFtiKnwHI4ckaXBpFKjrzxa2xVklK5tEcN7LQt6sv0eY_frIrYQz3mMfO1KEYRjhPvtgmwM2GDaokhcb-A6-dtP_KD5gm8uuzwnu1zlM_CDmdN54BTehKfcKwpFch8gJZgMXo_j2z3jyF7pCLaCCD9BNDb-WT3BNI1qsQ5EqjHULwwU1lYBZQ8-QszHjwc3s3-oTuxuPbi-u4q6pQjyVedrGThYOmaA2zlgp6ty6XGhhMU8bFIvWIn8VzkjlBIIUpneFjylSADf11DlTi89sp1k29pBByYlPbIZpnmwDa11YmSl8pxO5NpmJ2I-XSa4eg3dGhZqDwlH1wxGxc4rB613kee1fwJVQdSuhemslROz4JYJVtxFXFZfIp2WC1BOx09fLuIXofxHd2OXa30PYmpdlxFQv8r0B9a80swdvxq0kEiXPvrzHJzhiu5zkPFWkqGO20_5f26_IPK058cv7GYXVADk
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Rb9MwED5B98ILAgYiMJCREDxFS2wnTp5QN1oNREcFm7S3yE5smLQmY02F-u-5c9xCXshbYkexfL7cd-fzdwBvTeK41FzFmWzQQXHooJRcmViJAi1sIVLt6YsX5_nZpfx8lV2FgNs6pFXu_on-R910NcXIj7lEoFEmaL4-3P6KqWoU7a6GEhr34YCjp5BM4OBkdr78to-yJIjWVZIObI0C_fvjle1xdonSJhGcj6yRJ-0fIc1xnuQ_hmf-CB4GxMimg4gfwz3bPoHDaYve8mrL3jGfw-mD44ew_RjqnaDe3rCFH8QNnTtes-VQnHvNhqO5DqEnM1t2Oosvvs4X31lI2GKU-MF027Av3W_2iUa02gzJqmyuezZdUXEJdt3SHeJttHxsibP0FC7ns4vTszgUV4hrmad97GThEBs0xhkrRZNblwstLNprg06jtYjDCmekcgIBFZp5hVeNaICbpnbONOIZTNqutc-BlZxwis3Q3BN9YKMLKzOFbzqRa5OZCN7vJrm6HTg0KvQ9SBzVWBwRnJAM9r2I-9o_6O5-VEGVKl1rQ0diU5WW0lluSmL4KU3qMoGAUUdwtJNgFRRyXf1dPhG82TejKtH-iG5tt_F9CL7mZRmBGkl-NKBxS3v905NyK4nIkmcv_v_xl_CAk8NOOSfqCCb93ca-QlTTm9dh6f4B2df5jA
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Pb9MwFH6CceGCgIEIDPSQEJwCje3EyQFNZVs1EIVJrNJukd3Yo1KbQpuK9b_nPScdi7QDueWnLD877_uSz98DeGMHXigjdJyqigiKJ4JSCG1jLXPKsLlMTLAvHn_LTifqy0V68U__1HXg-lZqx_WkJqv5-6vf20Oa8B-ZcRJl_7BwDXUYu9QMaHjehXtCEUtnGV8H9du3MiGTUA1ZUFaLWSrUejje8ohejgpW_j382VdP3khHo4fwoMOROGwD_wjuuPox7A9r4tCLLb7FoOwMn8z3YXvcVUGh2TzHcWjEnFcjr_GsLdm9xnbBridAinaLRyfx-ffR-Ad2Mi5kOQiausKvyz_4mVu02LQSVhyZBocLLjmBs5r3CIVTPsSz2eX6CUxGJ-dHp3FXciGeqixpYq9yT4ihst46JavM-Uwa6SiLW6KSzhE6y71V2kuCWZT8NW1TwgjCVlPvbSWfwl69rN0zwEIwenEpgQA2FaxM7lSq6U4vM2NTG8G7XSeXv1pnjZIYCYej7Icjgk8cg-ur2BE7HFiuLstugpVmaiwvlE10UijvhC3Y96ewiU8lwUgTwcEuguVulJU0UERWDAgTRfD6-jRNMP5rYmq33IRrGNRmRRGB7kW-16D-mXr2M1h1a0V4U6TP_-PpL-C-YC7PchR9AHvNauNeEuBp7Kswkv8ClI__mQ
  priority: 102
  providerName: Scholars Portal
Title Differential Metabolomics Profiles Identified by CE-TOFMS between High and Low Intramuscular Fat Amount in Fattening Pigs
URI https://www.proquest.com/docview/2432690901
https://search.proquest.com/docview/2434057699
https://pubmed.ncbi.nlm.nih.gov/PMC7464425
https://doaj.org/article/acab065417194fe2b996969b1f53095a
Volume 10
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Ba9swFH60HYxeyrpuzF0XNBjbyU0syZZ9TLOEbsxd2FrozUi21AZipTQOI_9-T7JT6mt9MNiSQeg9-X1P-vQJ4IsaGcolFWHMK0xQDCYoGRUqFCzFCJuySHr54vwqubzhP2_j2z2Id3thPGm_VItzu6zP7eLecysf6nK444kN5_lEcIziNB7uwz466LMUvf39IgQZRa1AI8OUfljrBjvUqdiM0H0P4TVzq20iob1Y5CX7ezizz5J8FnZmb-Cow4tk3LbrGPa0fQsnY4u5cr0lX4lncPqp8RPYfu9OO8FRuyS5b8_S7Tpek3l7NPeatBtzDQJPorZkMg2vf8_yv6SjaxFH-yDSVuTX6h_54VpUb1qqKpnJhoxrd7QEWVj3hGgb4x6ZL-7W7-BmNr2eXIbd0QphyZOoCQ1PDSKDShmlOasSbRImmcZorTBl1BpRWGoUF4YhnMIgL_AqEQtQVZXGqIq9hwO7svoDkIw6lKJjDPZOPLCSqeaxwC8NS6SKVQDfdp1cPLQKGgVmHs4yRd8yAVw4GzzVcsrX_sXq8a7o7F_IUiq3ITYSUcaNpipz-j6ZikzMEC7KAM52Fiy64bguKEeUmo0Q-wTw-akYB5JbHZFWrza-jgOvSZYFIHqW7zWoX4Ie6iW5O488ffGXH-GQukzekVHEGRw0jxv9CeFOowbw6mJ6Nf8z8NMFeM95OvAu_x_hJASw
link.rule.ids 230,315,730,783,787,867,888,2109,2228,21400,24330,27936,27937,33756,33757,43817,53804,53806,74630
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Rb9MwELZge4AXxBiIwNiMhOApWmI7cfKEutGqg7ZU0El7i-zEHpPWZKypUP89d45blhfylthRLJ8v9935_B0hH3RkmVBMhomowEGx4KDkTOpQ8gwsbMZj5eiLp7N0fCm-XiVXPuC28mmV23-i-1FXTYkx8lMmAGjkEZivz3e_Q6wahburvoTGY7KPVFXgfO2fDWfzH7soSwRoXUZxx9bIwb8_XZoWZhcpbSLOWM8aOdL-HtLs50k-MDyj5-SZR4x00In4gDwy9QtyOKjBW15u6EfqcjhdcPyQbL74eiegt7d06gZxi-eOV3TeFede0e5orgXoSfWGng_DxffR9Cf1CVsUEz-oqis6af7QCxzRct0lq9KRaulgicUl6E2Nd4C3wfLROczSS3I5Gi7Ox6EvrhCWIo3b0IrMAjaotNVG8Co1NuWKG7DXGpxGYwCHZVYLaTkAKjDzEq4S0ADTVWmtrvgrslc3tXlNaM4Qp5gEzD3SB1YqMyKR8KblqdKJDsin7SQXdx2HRgG-B4qj6IsjIGcog10v5L52D5r768KrUqFKpfFIbCzjXFjDdI4MP7mObcIBMKqAHG0lWHiFXBX_lk9A3u-aQZVwf0TVplm7Pghf0zwPiOxJvjegfkt988uRcksByJIlb_7_8RPyZLyYTorJxezbW_KUofOO-SfyiOy192vzDhBOq4_9Mv4L2Vj8hg
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Bb9MwFLagkxAXNBiIwAAjIThFTWwnTk6o2xptsJYKNmm3yE5smLQmY0019d_znuMWciG3xIli-dl-37M_f4-QDzqyTCgmw0TUEKBYCFByJnUoeQYeNuOxcvLFs3l6eim-XCVXnv-08rTK7ZzoJuq6rXCNfMwEAI08Avc1tp4WsTgpPt_-DjGDFO60-nQaD8meFCmPRmTvaDpffN-tuESA3GUU98qNHGL98dJ00NIobxNxxgaeyQn4D1DnkDP5jxMq9skTjx7ppDf3U_LANM_IwaSByHm5oR-p43O6hfIDsjnxuU9gDN_QmavEDZ5BXtFFn6h7RftjuhZgKNUbejwNL74Vsx_Uk7cokkCoamp63t7TM6zRct0TV2mhOjpZYqIJet3gHWBv8IJ0Aa30nFwW04vj09AnWggrkcZdaEVmASfU2mojeJ0am3LFDfhuDQGkMYDJMquFtBzAFbh8CVcFyIDpurJW1_wFGTVtY14SmjPELCYB149SgrXKjEgkfGl5qnSiA_Jp28jlba-nUUIcguYoh-YIyBHaYPcW6mC7B-3dz9IPq1JVSuPx2FjGubCG6RzVfnId24QDeFQBOdxasPSDc1X-7UoBeb8rhmGFeyWqMe3avYNQNs3zgMiB5QcVGpY017-cQDf0PwFz4av___wdeQQ9uDw_m399TR4zjOORiiIPyai7W5s3AHY6_db34j9qZgDD
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Differential+Metabolomics+Profiles+Identified+by+CE-TOFMS+between+High+and+Low+Intramuscular+Fat+Amount+in+Fattening+Pigs&rft.jtitle=Metabolites&rft.au=Taniguchi%2C+Masaaki&rft.au=Arakawa%2C+Aisaku&rft.au=Nishio%2C+Motohide&rft.au=Okamura%2C+Toshihiro&rft.date=2020-08-07&rft.issn=2218-1989&rft.eissn=2218-1989&rft.volume=10&rft.issue=8&rft_id=info:doi/10.3390%2Fmetabo10080322&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2218-1989&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2218-1989&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2218-1989&client=summon