Fusion of cellulose binding domain from Trichoderma reesei CBHI to Cryptococcus sp. S-2 cellulase enhances its binding affinity and its cellulolytic activity to insoluble cellulosic substrates - Thongekkaew, Jantaporn; Ikeda, Hiroko; Masaki, Kazuo; Iefuji, Haruyuki (Enzyme and microbial technology, 2013)
Keywords: binding capacity; carbohydrate binding; Carbohydrate binding module; Carboxymethyl cellulase; Catalytic Domain; Cellulase - chemistry; Cellulase - genetics; Cellulase - metabolism; Cellulolytic activity; cellulose; Cellulose - metabolism; cellulose 1,4-beta-cellobiosidase; Cellulose binding domain; Cryptococcus; Cryptococcus - enzymology; Cryptococcus - genetics; Cryptococcus sp. S-2; endo-1,4-beta-glucanase; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Hypocrea jecorina; Insoluble cellulosic substrate; Protein Engineering; Protein Structure, Tertiary; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Solubility; Substrate Specificity; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma reesei; Insoluble cellulosic substrate; Carbohydrate binding module; Carboxymethyl cellulase; Cellulolytic activity; Cellulose binding domain; Cryptococcus sp. S-2