Immunofluorescence staining of adenovirus in fixed tissues pretreated with trypsin

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Published inJournal of Clinical Microbiology Vol. 17; no. 2; pp. 371 - 373
Main Authors Chandler, F W, Gorelkin, L
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.02.1983
Subjects
Adenoviridae - immunology
adenovirus
Animals
Antigens, Viral - analysis
Fluorescent Antibody Technique
Humans
Macaca mulatta
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AbstractList We describe an immunofluorescence staining procedure for adenovirus in Formalin-fixed, paraffin-embedded tissue sections pretreated with a 0.25% trypsin solution. In trypsinized tissue sections stained with fluorescein-labeled antibody to adenovirus, viral antigen was brightly fluorescent and easily detected each time specimens were processed, and the nonspecific background fluorescence was always minimal. Viral antigen was nonfluorescent or only weakly fluorescent in tissue sections not pretreated with trypsin. Because pathology laboratories usually receive Formalin-fixed tissues for examination, this rapid and practical procedure can be routinely used to extend the diagnostic capability of conventional histopathology.
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The authors describe an immunofluorescence staining procedure for adenovirus in Formalin-fixed, paraffin-embedded tissue sections pretreated with a 0.25% trypsin solution. In trypsinized tissue sections stained with fluorescein-labeled antibody to adenovirus, viral antigen was brightly fluorescent and easily detected each time specimens were processed, and the nonspecific background fluorescence was always minimal. Viral antigen was nonfluorescent or only weakly fluorescent in tissue sections not pretreated with trypsin. Because pathology laboratories usually receive Formalin-fixed tissues for examination, this rapid and practical procedure can be routinely used to extend the diagnostic capability of conventional histopathology.
Author F W Chandler
L Gorelkin
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We describe an immunofluorescence staining procedure for adenovirus in Formalin-fixed, paraffin-embedded tissue sections pretreated with a 0.25% trypsin...
The authors describe an immunofluorescence staining procedure for adenovirus in Formalin-fixed, paraffin-embedded tissue sections pretreated with a 0.25%...
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SubjectTerms Adenoviridae - immunology
adenovirus
Animals
Antigens, Viral - analysis
Fluorescent Antibody Technique
Humans
Macaca mulatta
Title Immunofluorescence staining of adenovirus in fixed tissues pretreated with trypsin
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