Stimulation and suppression of cell-mediated immunity by endosporulation antigens of Coccidioides immitis
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| Published in | Infection and Immunity Vol. 35; no. 2; pp. 431 - 436 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
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United States
American Society for Microbiology
01.02.1982
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| AbstractList | The culture filtrate of Coccidioides immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for ability to stimulate human lymphocyte blastogenesis in vitro. Stimulation of lymphocytes from skin test-positive healthy subjects by EA was comparable to stimulation by spherulin at optimal dilutions, but EA are 500 times more potent when compared on the basis of weight. Both preparations slightly stimulated lymphocytes from skin test-negative subjects. Heating or dialysis of EA enhanced the effect on skin test-positive subjects, but concentration depressed it. Concentrated EA also depressed nonspecific stimulation caused by phytohemagglutinin. Dialysis of concentrated EA reduced the ability to depress responses. EA from an avirulent strain of C. immitis were as stimulatory as EA from a virulent strain, but concentrating the former did not produce as much depression as concentrating the latter did. A survey of subjects with an optimal dose of EA in lymphocyte transformation showed that EA could separate skin test-positive from -negative subjects as well as spherulin could. The survey also showed that a delta cpm (the difference of incorporated counts of tritiated thymidine per minute in the presence or absence of the reagent) of 10,000 is useful for this separation. These results also indicate the presence of suppressive substances in EA which are only partially dialyzable and which were significantly more prominent in a preparation from the virulent strain. Classifications Services IAI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue IAI About IAI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy Connect to IAI IAI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0019-9567 Online ISSN: 1098-5522 Copyright © 2014 by the American Society for Microbiology. For an alternate route to IAI .asm.org, visit: IAI The culture filtrate of C. immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for ability to stimulate human lymphocyte blastogenesis in vitro. Stimulation of lymphocytes from skin test-positive healthy subjects by EA was comparable to stimulation by spherulin at optimal dilutions, but EA are 500 times more potent when compared on the basis of weight. Both preparations slightly stimulated lymphocytes from skin test-negative subjects. Dialysis of concentrated EA reduced the ability to depress responses. EA from an avirulent strain of C. immitis were as stimulatory as EA from a virulent strain, but concentrating the former did not produce as much depression as concentrating the latter did. A survey of subjects with an optimal dose of EA in lymphocyte transformation showed that EA could separate skin test-positive from -negative subjects as well as spherulin could. The survey also showed that a Delta cpm (the difference of incorporated counts of tritiated thymidine per minute in the presence or absence of the reagent) of 10,000 is useful for this separation. The culture filtrate of Coccidioides immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for ability to stimulate human lymphocyte blastogenesis in vitro. Stimulation of lymphocytes from skin test-positive healthy subjects by EA was comparable to stimulation by spherulin at optimal dilutions, but EA are 500 times more potent when compared on the basis of weight. Both preparations slightly stimulated lymphocytes from skin test-negative subjects. Heating or dialysis of EA enhanced the effect on skin test-positive subjects, but concentration depressed it. Concentrated EA also depressed nonspecific stimulation caused by phytohemagglutinin. Dialysis of concentrated EA reduced the ability to depress responses. EA from an avirulent strain of C. immitis were as stimulatory as EA from a virulent strain, but concentrating the former did not produce as much depression as concentrating the latter did. A survey of subjects with an optimal dose of EA in lymphocyte transformation showed that EA could separate skin test-positive from -negative subjects as well as spherulin could. The survey also showed that a delta cpm (the difference of incorporated counts of tritiated thymidine per minute in the presence or absence of the reagent) of 10,000 is useful for this separation. These results also indicate the presence of suppressive substances in EA which are only partially dialyzable and which were significantly more prominent in a preparation from the virulent strain.The culture filtrate of Coccidioides immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for ability to stimulate human lymphocyte blastogenesis in vitro. Stimulation of lymphocytes from skin test-positive healthy subjects by EA was comparable to stimulation by spherulin at optimal dilutions, but EA are 500 times more potent when compared on the basis of weight. Both preparations slightly stimulated lymphocytes from skin test-negative subjects. Heating or dialysis of EA enhanced the effect on skin test-positive subjects, but concentration depressed it. Concentrated EA also depressed nonspecific stimulation caused by phytohemagglutinin. Dialysis of concentrated EA reduced the ability to depress responses. EA from an avirulent strain of C. immitis were as stimulatory as EA from a virulent strain, but concentrating the former did not produce as much depression as concentrating the latter did. A survey of subjects with an optimal dose of EA in lymphocyte transformation showed that EA could separate skin test-positive from -negative subjects as well as spherulin could. The survey also showed that a delta cpm (the difference of incorporated counts of tritiated thymidine per minute in the presence or absence of the reagent) of 10,000 is useful for this separation. These results also indicate the presence of suppressive substances in EA which are only partially dialyzable and which were significantly more prominent in a preparation from the virulent strain. |
| Author | D A Stevens H B Levine C Brass |
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| References | 669811 - Infect Immun. 1978 May;20(2):541-51 14224511 - Science. 1964 Dec 25;146(3652):1648-54 81224 - J Immunol. 1978 Oct;121(4):1239-44 5375339 - Sabouraudia. 1969 Feb;7(1):20-32 14416255 - Trans N Y Acad Sci. 1960 Apr;22:436-49 4426703 - Infect Immun. 1974 Oct;10(4):700-4 991450 - Clin Exp Immunol. 1976 Jul;25(1):28-35 870430 - Infect Immun. 1977 Mar;15(3):751-5 500193 - Infect Immun. 1979 Sep;25(3):932-8 5786453 - J Immunol. 1969 May;102(5):1284-9 13732669 - J Infect Dis. 1961 Jan-Feb;108:35-44 13289883 - Proc Soc Exp Biol Med. 1955 Dec;90(3):709-11 |
| References_xml | – reference: 870430 - Infect Immun. 1977 Mar;15(3):751-5 – reference: 13732669 - J Infect Dis. 1961 Jan-Feb;108:35-44 – reference: 500193 - Infect Immun. 1979 Sep;25(3):932-8 – reference: 81224 - J Immunol. 1978 Oct;121(4):1239-44 – reference: 4426703 - Infect Immun. 1974 Oct;10(4):700-4 – reference: 5375339 - Sabouraudia. 1969 Feb;7(1):20-32 – reference: 991450 - Clin Exp Immunol. 1976 Jul;25(1):28-35 – reference: 669811 - Infect Immun. 1978 May;20(2):541-51 – reference: 14224511 - Science. 1964 Dec 25;146(3652):1648-54 – reference: 5786453 - J Immunol. 1969 May;102(5):1284-9 – reference: 13289883 - Proc Soc Exp Biol Med. 1955 Dec;90(3):709-11 – reference: 14416255 - Trans N Y Acad Sci. 1960 Apr;22:436-49 |
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Reddit... The culture filtrate of Coccidioides immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for... The culture filtrate of C. immitis induced to replicate in its parasitic phase in vitro, termed endosporulation antigens (EA), was assayed for ability to... |
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| StartPage | 431 |
| SubjectTerms | Animals Antigens, Fungal - immunology Coccidioides - immunology Coccidioides immitis Coccidioidin - immunology Coccidioidomycosis - immunology Dialysis Dose-Response Relationship, Immunologic Hot Temperature Humans Lymphocyte Activation Skin Tests Spores, Fungal - immunology |
| Title | Stimulation and suppression of cell-mediated immunity by endosporulation antigens of Coccidioides immitis |
| URI | http://iai.asm.org/content/35/2/431.abstract https://www.ncbi.nlm.nih.gov/pubmed/7056572 https://www.proquest.com/docview/15350030 https://www.proquest.com/docview/74018496 https://pubmed.ncbi.nlm.nih.gov/PMC351057 |
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