Evaluation of cell segmentation methods without reference segmentations

• Published in: Molecular biology of the cell Vol. 34; no. 6; p. ar50
• Main Authors: Chen, Haoran, Murphy, Robert F.
• Format: Journal Article
• Language: English
• Published: United States American Society for Cell Biology 15.05.2023; The American Society for Cell Biology
• Subjects: Analysis; Cells; Methods; Public software; Segmentation (Morphology); United States

We developed metrics and an open source tool to evaluate the cell segmentation quality of multichannel images without a human-labeled reference. Deep learning–based methods performed the best, but postprocessing to ensure proper matching of cell and nuclear masks dramatically improved performance. C...