Bifunctional chimeric fusion proteins engineered for DNA delivery: Optimization of the protein to DNA ratio

• Published in: Biochimica et biophysica acta Vol. 1790; no. 3; pp. 198 - 207
• Main Authors: Gao, Shan, Simon, Melissa J., Morrison, Barclay, Banta, Scott
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2009
• Subjects: Animals; Base Sequence; Cell penetrating peptide; DNA - administration & dosage; DNA - metabolism; DNA Primers; Electrophoresis, Agar Gel; Flow Cytometry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Oligonucleotide delivery; p50 DNA-binding domain; PC12 Cells; Plasmids; Protein Engineering; Protein/DNA ratio; Rats; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; TAT; Transgenes; p50 DNA-binding domain; Cell penetrating peptide; TAT; Oligonucleotide delivery; Protein/DNA ratio

Cell penetrating peptides (CPPs) have been used to deliver nucleotide-based therapeutics to cells, but this approach has produced mixed results. Ionic interactions and covalent bonds between the CPPs and the cargos may inhibit the effectiveness of the CPPs or interfere with the bioactivity of the ca...